KEGG   ENZYME: 1.14.19.36
Entry
EC 1.14.19.36               Enzyme                                 
Name
sn-1 acyl-lipid omega-3 desaturase (ferredoxin);
desB (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
1-gamma-linolenoyl-2-acyl-[glycerolipid],ferredoxin:oxygen oxidoreductase (15,16 cis-dehydrogenating)
Reaction(IUBMB)
(1) a 1-gamma-linolenoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = a 1-stearidonoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O;
(2) a 1-linoleoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = a 1-alpha-linolenoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
Substrate
1-gamma-linolenoyl-2-acyl-[glycerolipid];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
O2 [CPD:C00007];
H+ [CPD:C00080];
1-linoleoyl-2-acyl-[glycerolipid]
Product
1-stearidonoyl-2-acyl-[glycerolipid];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001];
1-alpha-linolenoyl-2-acyl-[glycerolipid]
Comment
The enzyme, characterized from cyanobacteria, introduces a cis double bond at carbon 15 of linoleoyl and gamma-linolenoyl groups attached to the sn-1 position of glycerolipids. The enzyme is an omega desaturase, and determines the location of the double bond by counting three carbons from the methyl end of the fatty acid. It is nonspecific with respect to the polar head group of the glycerolipid. cf. EC 1.14.19.35, sn-2 acyl-lipid omega-3 desaturase (ferredoxin).
History
EC 1.14.19.36 created 2015
Orthology
K10257  acyl-lipid omega-3 desaturase
Genes
AJM: 119045597
LSM: 121115619 121115925
CEL: CELE_W02A2.1(fat-2) CELE_Y67H2A.8(fat-1)
CBR: CBG_01790(Cbr-fat-2) CBG_01791(Cbr-fat-1)
CRQ: GCK72_014531 GCK72_014532
BMY: BM_BM6111(Bm6111)
LOA: LOAG_02175
HRO: HELRODRAFT_71572
LGI: LOTGIDRAFT_116269 LOTGIDRAFT_74451
PCAN: 112555112
HRF: 124116168
MMER: 123555506
DPOL: 127834109
OBI: 106883679
OSN: 115215637
ATH: AT2G29980(FAD3) AT3G11170(FAD7) AT5G05580(FAD8)
ALY: 9307241 9315309 9320901
CRB: 17881387 17889809 17891716
CSAT: 104700502 104708554 104734563 104745318 104749896 104764868 104765502 104768754 104786676
EUS: EUTSA_v10013625mg EUTSA_v10016786mg EUTSA_v10020755mg
BRP: 103850591 103857278 103859381 103864998 103867994 103870371
BNA: 106353041 106377255 106380841 106387896 106418558 106422764 106429840 106439274 106446979 106452545 111197728 125584238 125585240 125595996
BOE: 106295700 106314490 106327053 106328829 106335120 106336554 106336680
RSZ: 108811531 108837004 108843138 108844225 108848571 108859156 108862810
THJ: 104800741 104816198 104816543 104826849
CPAP: 110808059 110810025 110820958
CIT: 102611986 102626347
CIC: CICLE_v10011691mg CICLE_v10028941mg
PVY: 116115152 116121787 116122546 116129441 116129513
MINC: 123192198 123201556 123209054 123209055 123228271
TCC: 18585841 18590479 18597527
GRA: 105776289 105780050 105786551 105791965 105799158
GHI: 107889065 107891556 107891614 107899542 107931808 107940880 107948941 107956656 107958053 121207887
GAB: 108454561 108457033 108461005 108464318 108466542 108480912
DZI: 111285613 111293917 111296353 111304348 111310113 111312056
EGR: 104421068 104421444
GMX: 100037456(FAD3B) 100217335(FAD8-1) 100305351(FAD7-1) 100499626(FAD7-2) 100799517(FAD8.2) 547470(FAD3C) 547715(FAD3A) 732540(FAD3-2b)
GSJ: 114374933 114383326 114394696 114395027 114396629 114406122 114409044 114416093 114419078
PVU: PHAVU_006G068600g PHAVU_008G098100g PHAVU_008G260700g PHAVU_010G076200g
VRA: 106759364 106765282 106774181 106775023
VAR: 108329591 108337050 108341784 108347618
VUN: 114167372 114185384 114186845 114193336
CCAJ: 109787870 109813625 109815393 109816410
APRC: 113846079 113849137 113851549 113863868
MTR: 11411563 11415576 25490906 25498767
TPRA: 123889397 123892471 123914473 123922449 123924861
CAM: 101502461 101504225 101513464 101515266
PSAT: 127084563 127107471 127118542 127127449
LJA: Lj0g3v0123589.1(Lj0g3v0123589.1) Lj1g3v2337910.1(Lj1g3v2337910.1) Lj2g3v2277520.1(Lj2g3v2277520.1)
ADU: 107458340 107475149 107484970 107491190
AIP: 107609767 107625758 107639562 107644755
AHF: 112702815 112720001 112728003 112743658 112750362 112766655 112792318 112797044
LANG: 109330327 109338970 109349642 109350266 109352644 109360270 109361525
PCIN: 129289510 129290769 129298447
FVE: 101306021 101306861 101310644 101312263
RCN: 112165678 112182995 112193656
PPER: 18772228 18773198 18774962 18785351
PMUM: 103320961 103320972 103333043 103341784
PAVI: 110749762 110758660 110758749 110770476
PDUL: 117613027 117617094 117631111 117631112 117632464
MDM: 103419381 103420048 103430794 103432837 103447381 103447641
MSYL: 126590692 126590694 126592394 126603049 126616550 126617350 126617712 126629706
PXB: 103930542 103931220 103931253 103936863 103938921 103958619 103966319
ZJU: 107404190 107410758 107410778 107430888
MNT: 21385286 21391077
CSAV: 115697681 115705594 115720710
CSV: 101209295 101223135(FAD7)
CMO: 103497071 103502871
BHJ: 120081471 120082405
MCHA: 111023722
CMAX: 111469542 111489597 111491157 111492652
CMOS: 111447744 111452212 111458375 111464727
CPEP: 111778662 111782701 111787876 111806684
RCU: 8265360 8286343 8286466
JCU: 105630235(FAD3) 105646096(FAD) 105646580
HBR: 110648982 110654511 110654544 110657237 110665331 110666427
MESC: 110613473 110619516 110619524 110620027 110624941
POP: 7466077 7468059 7478523 7490323 7494502
PEU: 105111171 105121928 105133573 105139146 105142210
PALZ: 118033024 118037588 118043346 118048506 118055844
JRE: 108989903 108989905 109002248 109007160 109015144
CILL: 122276706 122290752 122293355 122301503
QSU: 112005607 112011944 112030173 112031015
QLO: 115952967 115989685
TWL: 119998266 120001334 120012033
VVI: 100243349 100247097 100251798 100260414
VRI: 117915999 117917133 117921171 117928747
SLY: 100134915 544203(Fad7)
SPEN: 107021758 107023114 107023926
SOT: 102596735 102597287 102597672
SSTN: 125872918 125872941
CANN: 107873310 107873419 107873420
NTA: 107763446(FAD3) 107777511 107783156(NtFAD7) 107786526(NtFAD3-2) 107804927 107807128 107832399
NSY: 104237848 104243629 104243898 104248927
NTO: 104090878 104112653 104114074
NAU: 109213818 109214388 109238135
INI: 109147953 109164374 109186614
ITR: 116000429 116013334 116014945
SIND: 105160895 105163949 105167042
OEU: 111373879 111401332 111403039 111403247
EGT: 105960105 105969857 105977522
SSPL: 121748592 121762807 121763363 121774873 121777347 121779911 121782532 121786158 121791991
SMIL: 130992039 131003346 131005695
APAN: 127244856 127251442
HAN: 110868642 110868972 110872089 110872729 110878910 110885030 110907224 110922364
ECAD: 122582349 122594010 122600878 122602668 122606927
LSV: 111882696 111893204 111901267 111912570 111919702
CCAV: 112506819 112512083 112514077 112518049 112526060
DCR: 108192445 108206807 108209044 108210458 108219754
CSIN: 114283960 114287210 114292016 114315720 114318519
RVL: 131314300 131320560 131323256 131323270 131333756
BVG: 104903256 104903266
SOE: 110801682 110801700
CQI: 110699288 110699289 110733453 110733454
ATRI: 130803064 130803067 130803850
MOF: 131143500 131147647 131149084 131149085
NNU: 104594909 104602583
MING: 122085160 122089304
TSS: 122651879 122669968 122670968
PSOM: 113309945 113310323 113320193 113338748
OSA: 4332500 4344389 4349544 4351253
DOSA: Os03t0290300-01(Os03g0290300) Os07t0693800-01(Os07g0693800) Os11t0104400-01(Os11g0104400) Os12t0104400-01(Os12g0104400)
OBR: 102707070 102715194 102720167 102720386
OGL: 127754012 127756827 127768014 127779695
BDI: 100824939 100831377 100840287
ATS: 109748184 109753536 109765018 109778723
TDC: 119285393 119286162 119290627 119295655 119301018 119308532 119353119 119362211
TAES: 123051381 123085571 123086342 123091705 123092458 123096668 123097784 123103230 123120533 123187329 543187 543189
TUA: 125508245 125508814 125535517 125552086
LPER: 127294720 127295843 127300174 127331350
LRD: 124652208 124652514 124678689 124699202 124702263
SBI: 8055482 8072903 8079958 8084033 8084992
ZMA: 100281245 100283566 103644592 542222
SITA: 101771745 101776425 101785330
SVS: 117834208 117839178 117863610
PVIR: 120643370 120652727 120686119 120689735 120694048 120696494
PHAI: 112875540 112893600
PDA: 103706691 103709505 103718325
EGU: 105044596 105046171 105057100
MUS: 103972340 103984918 103986078 103994000 103995423 103996941 103999859
ZOF: 121983175 121987737 121989428 121998376 122000948 122005229 122020419 122023714 122035993 122036024
DCT: 110105024 110110723 110110724
PEQ: 110023217 110023647 110023671
AOF: 109820564 109849018
NCOL: 116254277
ATR: 18433434 18442202
SMO: SELMODRAFT_103593 SELMODRAFT_109519
PPP: 112277833 112279588 112290291 112290971 112294054 112294506
CRE: CHLRE_01g038600v5
VCN: VOLCADRAFT_76038
MNG: MNEG_12033 MNEG_15487 MNEG_9648
CSL: COCSUDRAFT_67054
CVR: CHLNCDRAFT_33387
APRO: F751_6504
MIS: MICPUN_69560
MPP: MICPUCDRAFT_3762
GSL: Gasu_25760
CCP: CHC_T00009454001
PTI: PHATRDRAFT_41570(PTD15)
FCY: FRACYDRAFT_138896
EHX: EMIHUDRAFT_468588
PCAM: HNE05_09660
MOQ: HWV03_03565
SYN: sll1441(desB)
SYZ: MYO_117450(desB)
SYY: SYNGTS_1727(desB)
SYT: SYNGTI_1727(desB)
SYS: SYNPCCN_1726(desB)
SYQ: SYNPCCP_1726(desB)
SYO: C7I86_09000
SYP: SYNPCC7002_A0159(desB)
SYNP: Syn7502_00126
SYV: AWQ23_00965
SYL: AWQ21_00800
CMP: Cha6605_3989
AMR: AM1_3759
WNA: KA717_09260
LEP: Lepto7376_2407
LEN: LEP3755_23000
LBO: LBWT_51340
PSER: ABRG53_0325
PGX: OA858_16045
MAR: MAE_34620(desB)
MPK: VL20_3142
MIQ: B5D77_03815
MVZ: myaer102_09850(desB)
CYT: cce_4388
CWA: CwatDRAFT_3625
CYN: Cyan7425_3182
TER: Tery_4492
PAGH: NIES204_40690
PPSU: NO713_04408(DES3)
OXY: HCG48_05415
MVAG: D0A34_26480
MIC: Mic7113_4950
GEI: GEI7407_2532
OAC: Oscil6304_4510
ONI: Osc7112_5416
CEP: Cri9333_2131
ANA: all1597
NPU: Npun_R6230
NOS: Nos7107_0616
NOP: Nos7524_4758
NON: NOS3756_14570
NFL: COO91_07298
NOE: CLI64_08215
NSH: GXM_01597
NED: HUN01_29405
AVA: Ava_4210
NAZ: Aazo_3556
ANB: ANA_C20204
ACY: Anacy_4778
AWA: AA650_16760
ANN: EH233_10955
CSG: Cylst_2674
RSIN: B6N60_02679
CALO: Cal7507_2591
CALT: Cal6303_1446
CALH: IJ00_05015
RIV: Riv7116_4399
NSPH: BDGGKGIB_02539(desA_1)
DOU: BMF77_00181(desA_2)
DFS: HGD76_17195
DHT: NG743_21980
CCUR: IAR63_05435
CRK: L3I90_06310
AFLO: HEQ12_18925
TOQ: HCG51_19355
NCN: BZZ01_14805
CTHE: Chro_2759
LKB: LPTSP3_g23450(desA_1)
 » show all
Reference
1  [PMID:7524725]
  Authors
Sakamoto T, Los DA, Higashi S, Wada H, Nishida I, Ohmori M, Murata N
  Title
Cloning of omega 3 desaturase from cyanobacteria and its use in altering the degree of membrane-lipid unsaturation.
  Journal
Plant Mol Biol 26:249-63 (1994)
DOI:10.1007/BF00039536
  Sequence
[syn:sll1441]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.36
IUBMB Enzyme Nomenclature: 1.14.19.36
ExPASy - ENZYME nomenclature database: 1.14.19.36
BRENDA, the Enzyme Database: 1.14.19.36

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Protein sequence (10)   
   UniProt (10)   
DNA sequence (8)   
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