KEGG   ENZYME: 1.14.19.38
Entry
EC 1.14.19.38               Enzyme                                 
Name
acyl-lipid Delta6-acetylenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
Delta6 acyl-lipid,ferrocytochrome-b5:oxygen oxidoreductase (6,7-dehydrogenating)
Reaction(IUBMB)
(1) a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a (9Z,12Z)-octadeca-9,12-dien-6-ynoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O;
(2) a stearidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a (9Z,12Z,15Z)-octadeca-9,12,15-trien-6-ynoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
Substrate
gamma-linolenoyl-[glycerolipid];
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080];
stearidonoyl-[glycerolipid]
Product
(9Z,12Z)-octadeca-9,12-dien-6-ynoyl-[glycerolipid];
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001];
(9Z,12Z,15Z)-octadeca-9,12,15-trien-6-ynoyl-[glycerolipid]
Comment
The enzyme, characterized from the moss Ceratodon purpureus, converts the double bond at position 6 of gamma-linolenate and stearidonate into a triple bond. The product of the latter, dicranin, is the main fatty acid found in C. purpureus. The enzyme contains a cytochrome b5 domain that acts as the direct electron donor to the desaturase active site. The enzyme also has the activity of EC 1.14.19.47, acyl-lipid (9-3)-desaturase.
History
EC 1.14.19.38 created 2015
Orthology
K21737  acyl-lipid Delta6-acetylenase / acyl-lipid (9-3)-desaturase
Genes
BFO118409704 118409836
BBEL109462408 109469769
CIN100181774
SCLV120339347
LOALOAG_10800
NAINECAME_05225
LAK106165720 106175792 106175795
NVE5520800
AMIL114962826
PDAM113673085
SPIS111333863
TADTRIADDRAFT_28957
THJ104803726
CPAP110822096
CIT102624404
CICCICLE_v10010195mg
PVY116143292
MINC123195209 123201006
TCC18588068
GRA105769952 105792967
GHI107907009 107932872 107958876 121204795
GAB108459943 108467416
DZI111285207 111285209 111300415 111304116
EGR104436303
GMX100775880(SLD1.4) 100776476(SLD1.5)
GSJ114385103 114393364
PVUPHAVU_001G036200g
VRA106765081
VAR108336250
VUN114195260
CCAJ109795310
APRC113846413
MTRMTR_1g016060 MTR_1g016070
CAM101502873
LJALj0g3v0112719.1(Lj0g3v0112719.1) Lj5g3v0405030.1(Lj5g3v0405030.1)
ADU107461234 107495639
AIP107605498 107606314
AHF112697662 112706370 112755914 112766680
LANG109328522 109343827
FVE101310770
RCN112173700
PPER18789224
PMUM103320564
PAVI110764665
PDUL117616024
MDM103440767
PXB103952139
ZJU107420047
MNT21402436
RCU8259572
JCU105638142
HBR110633287
MESC110604075 110610280
POP7462256
PEU105122191
PALZ118030785
JRE109021249
QLO115970363
TWL120005399
VVI100259521
VRI117913126
SLY101257882
SPEN107028997
SOT102599871
CANN107864646 107867549
NTA107768418 107798633(DXDES)
NSY104239037 104239380
NTO104121325
NAU109217138
INI109158372 109158482 109191355
ITR116005129 116005533 116006009
SIND105164669 105176919
OEU111373557 111375208
EGT105977192
SSPL121780413 121782620
HAN110941481
ECAD122579280
LSV111906061
CCAV112504110
CSIN114300284 114319212
BVG104886626
NNU104593449 104600267
MING122061951 122082529
TSS122648698 122664603
PSOM113299171 113322696 113340082 113343074 113358398
NCOL116267278
OSA4346776
DOSAOs09t0338500-01(Os09g0338500)
OBR102714763
BDI100826592
ATS109751130
TDC119301325 119308865
TAES123103524 123121294 542825
SBI8054590 8075008
ZMA100191970 100279397 103651619
SITA101761789 101764528
SVS117845251 117855905
PVIR120660411 120676547
PHAI112882473
PDA103710903 103715999 103718541
EGU105040770 105041773 105048251
MUS103978403 103988108 103996075
DCT110096312 110112964 110112966 110116583
PEQ110020235 110035062 110037593
AOF109827972 109844045
ATR18436755
SMOSELMODRAFT_100447 SELMODRAFT_231254
PPP112277136 112282380 112284131
CSLCOCSUDRAFT_47604
MISMICPUN_109534
MPPMICPUCDRAFT_48880
CCPCHC_T00008879001
TETTTHERM_00138530 TTHERM_00339850
PTMGSPATT00028900001
SMINv1.2.012127.t1(symbB.v1.2.012127.t1)
PTIPHATRDRAFT_29488(PTD6)
FCYFRACYDRAFT_228533(FAD2)
TPSTHAPSDRAFT_41113(DES3)
AAFAURANDRAFT_28278
PIFPITG_05127
PSOJPHYSODRAFT_356130
SPARSPRG_06919
LIFLINJ_36_7290
LDOLDBPK_367290
LMILMXM_36_6950
LBZLBRM_35_7330
LPANLPMP_357160
 » show all
Reference
1  [PMID:10848999]
  Authors
Sperling P, Lee M, Girke T, Zahringer U, Stymne S, Heinz E
  Title
A bifunctional delta-fatty acyl acetylenase/desaturase from the moss Ceratodon purpureus. A new member of the cytochrome b5 superfamily.
  Journal
Eur J Biochem 267:3801-11 (2000)
DOI:10.1046/j.1432-1327.2000.01418.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.38
IUBMB Enzyme Nomenclature: 1.14.19.38
ExPASy - ENZYME nomenclature database: 1.14.19.38
BRENDA, the Enzyme Database: 1.14.19.38

DBGET integrated database retrieval system