KEGG   ENZYME: 1.14.19.38
Entry
EC 1.14.19.38               Enzyme                                 
Name
acyl-lipid Delta6-acetylenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
Delta6 acyl-lipid,ferrocytochrome-b5:oxygen oxidoreductase (6,7-dehydrogenating)
Reaction(IUBMB)
(1) a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a (9Z,12Z)-octadeca-9,12-dien-6-ynoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O;
(2) a stearidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a (9Z,12Z,15Z)-octadeca-9,12,15-trien-6-ynoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
Substrate
gamma-linolenoyl-[glycerolipid] [CPD:C22563];
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080];
stearidonoyl-[glycerolipid] [CPD:C22564]
Product
(9Z,12Z)-octadeca-9,12-dien-6-ynoyl-[glycerolipid];
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001];
(9Z,12Z,15Z)-octadeca-9,12,15-trien-6-ynoyl-[glycerolipid]
Comment
The enzyme, characterized from the moss Ceratodon purpureus, converts the double bond at position 6 of gamma-linolenate and stearidonate into a triple bond. The product of the latter, dicranin, is the main fatty acid found in C. purpureus. The enzyme contains a cytochrome b5 domain that acts as the direct electron donor to the desaturase active site. The enzyme also has the activity of EC 1.14.19.47, acyl-lipid (9-3)-desaturase.
History
EC 1.14.19.38 created 2015
Orthology
K21737  acyl-lipid Delta6-acetylenase / acyl-lipid (9-3)-desaturase
Genes
BFO: 118409704 118409836
BBEL: 109462408 109469769
CIN: 100181774
SCLV: 120339347
LOA: LOAG_10800
NAI: NECAME_05225
LAK: 106165720 106175792 106175795
NVE: 5520800
AMIL: 114962826
PDAM: 113673085
SPIS: 111333863
TAD: TRIADDRAFT_28957
THJ: 104803726
CPAP: 110822096
CIT: 102624404
CIC: CICLE_v10010195mg
PVY: 116143292
MINC: 123195209 123201006
TCC: 18588068
GRA: 105769952 105792967
GHI: 107907009 107932872 107958876 121204795
GAB: 108459943 108467416
DZI: 111285207 111285209 111300415 111304116
EGR: 104436303
GMX: 100775880(SLD1.4) 100776476(SLD1.5)
GSJ: 114385103 114393364
PVU: PHAVU_001G036200g
VRA: 106765081
VAR: 108336250
VUN: 114195260
CCAJ: 109795310
APRC: 113846413
MTR: 112419175 11416346
TPRA: 123916771
CAM: 101502873
PSAT: 127097864
LJA: Lj0g3v0112719.1(Lj0g3v0112719.1) Lj5g3v0405030.1(Lj5g3v0405030.1)
ADU: 107461234 107495639
AIP: 107605498 107606314
AHF: 112697662 112706370 112755914 112766680
LANG: 109328522 109343827
PCIN: 129299122 129303299 129317564
FVE: 101310770
RCN: 112173700
PPER: 18789224
PMUM: 103320564
PAVI: 110764665
PDUL: 117616024
MDM: 103440767
MSYL: 126631793
PXB: 103952139
ZJU: 107420047
MNT: 21402436
CSAV: 115707010
RCU: 8259572
JCU: 105638142
HBR: 110633287
MESC: 110604075 110610280
POP: 7462256
PEU: 105122191
PALZ: 118030785
JRE: 109021249
CILL: 122297210
QLO: 115970363
TWL: 120005399
VVI: 100259521
VRI: 117913126
SLY: 101257882
SPEN: 107028997
SOT: 102599871
SSTN: 125841119
CANN: 107864646 107867549
NTA: 107768418 107798633(DXDES)
NSY: 104239037 104239380
NTO: 104121325
NAU: 109217138
INI: 109158372 109158482 109191355
ITR: 116005129 116005533 116006009
SIND: 105164669 105176919
OEU: 111373557 111375208
EGT: 105977192
SSPL: 121780413 121782620
APAN: 127265509
HAN: 110941481
ECAD: 122579280
LSV: 111906061
CCAV: 112504110
CSIN: 114300284 114319212
BVG: 104886626
NNU: 104593449 104600267
MING: 122061951 122082529
TSS: 122648698 122664603
PSOM: 113299171 113322696 113340082 113343074 113358398
OSA: 4346776
DOSA: Os09t0338500-01(Os09g0338500)
OBR: 102714763
OGL: 127785481
BDI: 100826592
ATS: 109751130
TDC: 119301325 119308865
TAES: 123103524 123121294 542825
TUA: 125509111
LPER: 127300755
SBI: 8054590 8075008
ZMA: 100191970 100279397 103651619
SITA: 101761789 101764528
SVS: 117845251 117855905
PVIR: 120660411 120676547
PHAI: 112882473
PDA: 103710903 103715999 103718541
EGU: 105040770 105041773 105048251
MUS: 103978403 103988108 103996075
ZOF: 121978954 121982615 121987241 122037872
DCT: 110096312 110112964 110112966 110116583
PEQ: 110020235 110035062 110037593
AOF: 109827972 109844045
NCOL: 116267278
ATR: 18436755
SMO: SELMODRAFT_100447 SELMODRAFT_231254
PPP: 112277136 112282380 112284131
CSL: COCSUDRAFT_47604
MIS: MICPUN_109534
MPP: MICPUCDRAFT_48880
CCP: CHC_T00008879001
TET: TTHERM_00138530 TTHERM_00339850
PTM: GSPATT00028900001
SMIN: v1.2.012127.t1(symbB.v1.2.012127.t1)
PTI: PHATRDRAFT_29488(PTD6)
FCY: FRACYDRAFT_228533(FAD2)
TPS: THAPSDRAFT_41113(DES3)
AAF: AURANDRAFT_28278
PIF: PITG_05127
PSOJ: PHYSODRAFT_356130
SPAR: SPRG_06919
LIF: LINJ_36_7290
LDO: LDBPK_367290
LMI: LMXM_36_6950
LBZ: LBRM_35_7330
LPAN: LPMP_357160
 » show all
Reference
1  [PMID:10848999]
  Authors
Sperling P, Lee M, Girke T, Zahringer U, Stymne S, Heinz E
  Title
A bifunctional delta-fatty acyl acetylenase/desaturase from the moss Ceratodon purpureus. A new member of the cytochrome b5 superfamily.
  Journal
Eur J Biochem 267:3801-11 (2000)
DOI:10.1046/j.1432-1327.2000.01418.x
  Sequence
[ag:CAB94992]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.38
IUBMB Enzyme Nomenclature: 1.14.19.38
ExPASy - ENZYME nomenclature database: 1.14.19.38
BRENDA, the Enzyme Database: 1.14.19.38

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Gene (3988)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (210)   
   KEGG MGENES (91)   
   RefGene (3686)   
Protein sequence (2)   
   UniProt (1)   
   SWISS-PROT (1)   
Protein domain (4)   
   InterPro (4)   
All databases (3994)   

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