KEGG   ENZYME: 1.14.19.39
Entry
EC 1.14.19.39               Enzyme                                 
Name
acyl-lipid Delta12-acetylenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
Delta12 acyl-lipid,ferrocytochrome-b5:oxygen oxidoreductase (12,13-dehydrogenating)
Reaction(IUBMB)
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = crepenynyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
Reaction(KEGG)
(other) R05740
Substrate
linoleoyl-[glycerolipid] [CPD:C21529];
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080]
Product
crepenynyl-[glycerolipid];
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the plant Crepis alpina, converts the double bond at position 12 of linoleate into a triple bond. The product is the main fatty acid found in triacylglycerols in the seed oil of Crepis alpina.
History
EC 1.14.19.39 created 2000 as EC 1.14.99.33, transferred 2015 to EC 1.14.19.39
Pathway
ec00591  Linoleic acid metabolism
Orthology
K08262  acyl-lipid delta12-acetylenase
Reference
1
  Authors
Banas A, Bafor M, Wiberg E, Lenman M, Staahl U, Stymne S.
  Title
Biosynthesis of an acetylenic fatty acid in microsomal preparations from developing seeds Crepis alpina.
  Journal
Physiol Biochem Mol Biol Plant [Proc Int Symp Plant Lipids] 12th:57-59 (1997)
Reference
2  [PMID:9572738]
  Authors
Lee M, Lenman M, Banas A, Bafor M, Singh S, Schweizer M, Nilsson R, Liljenberg C, Dahlqvist A, Gummeson PO, Sjodahl S, Green A, Stymne S
  Title
Identification of non-heme diiron proteins that catalyze triple bond and epoxy group formation.
  Journal
Science 280:915-8 (1998)
DOI:10.1126/science.280.5365.915
  Sequence
[ag:CAA76158]
Reference
3  [PMID:17329262]
  Authors
Nam JW, Kappock TJ
  Title
Cloning and transcriptional analysis of Crepis alpina fatty acid desaturases affecting the biosynthesis of crepenynic acid.
  Journal
J Exp Bot 58:1421-32 (2007)
DOI:10.1093/jxb/erm005
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.39
IUBMB Enzyme Nomenclature: 1.14.19.39
ExPASy - ENZYME nomenclature database: 1.14.19.39
BRENDA, the Enzyme Database: 1.14.19.39

  All links  
Protein sequence (39)   
   UniProt (36)   
   SWISS-PROT (1)   
   RefSeq(pep) (2)   
DNA sequence (24)   
   RefSeq(nuc) (2)   
   GenBank (14)   
   EMBL (8)   
Protein domain (2)   
   InterPro (2)   
All databases (65)   

Download RDF
DBGET integrated database retrieval system