| Entry |
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| Name |
acyl-lipid Delta12-acetylenase
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| Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
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| Sysname |
Delta12 acyl-lipid,ferrocytochrome-b5:oxygen oxidoreductase (12,13-dehydrogenating)
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| Reaction(IUBMB) |
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = crepenynyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
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| Reaction(KEGG) |
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| Substrate |
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| Product |
crepenynyl-[glycerolipid];
ferricytochrome b5 [CPD: C00996];
H2O [CPD: C00001]
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| Comment |
The enzyme, characterized from the plant Crepis alpina, converts the double bond at position 12 of linoleate into a triple bond. The product is the main fatty acid found in triacylglycerols in the seed oil of Crepis alpina.
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| History |
EC 1.14.19.39 created 2000 as EC 1.14.99.33, transferred 2015 to EC 1.14.19.39
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| Pathway |
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| Orthology |
| K08262 | acyl-lipid delta12-acetylenase |
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| Reference |
1 |
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| Authors |
Banas, A., Bafor, M., Wiberg, E., Lenman, M., Staahl, U. and Stymne, S. |
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| Title |
Biosynthesis of an acetylenic fatty acid in microsomal preparations from developing seeds Crepis alpina. |
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| Journal |
Physiol Biochem Mol Biol Plant [Proc Int Symp Plant Lipids] 12th:57-59 (1997) |
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| Reference |
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| Authors |
Lee M, Lenman M, Banas A, Bafor M, Singh S, Schweizer M, Nilsson R, Liljenberg C, Dahlqvist A, Gummeson PO, Sjodahl S, Green A, Stymne S. |
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| Title |
Identification of non-heme diiron proteins that catalyze triple bond and epoxy group formation. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Nam JW, Kappock TJ |
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| Title |
Cloning and transcriptional analysis of Crepis alpina fatty acid desaturases affecting the biosynthesis of crepenynic acid. |
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| Journal |
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| Other DBs |
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