KEGG ENZYME: 1.14.19.41

ENZYME: 1.14.19.41

Entry

EC 1.14.19.41               Enzyme

Name

sterol 22-desaturase;
ERG5 (gene name);
CYP710A (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water

Sysname

ergosta-5,7,24(28)-trien-3beta-ol,NADPH:oxygen oxidoreductase (22,23-dehydrogenating)

Reaction(IUBMB)

ergosta-5,7,24(28)-trien-3beta-ol + NADPH + H+ + O2 = ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADP+ + 2 H2O [RN:R07506]

Reaction(KEGG)

R07506;
(other) R07489 R11097

Substrate

ergosta-5,7,24(28)-trien-3beta-ol [CPD:C15780];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

ergosta-5,7,22,24(28)-tetraen-3beta-ol [CPD:C05440];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Comment

A heme-thiolate protein (P-450). The enzyme, found in yeast and plants, catalyses the introduction of a double bond between the C-22 and C-23 carbons of certain sterols. In yeast the enzyme acts on ergosta-5,7,24(28)-trien-3beta-ol, a step in the biosynthesis of ergosterol. The enzyme from the plant Arabidopsis thaliana acts on sitosterol and 24-epi-campesterol, producing stigmasterol and brassicasterol, respectively.

History

EC 1.14.19.41 created 2015

Pathway

ec00100

Steroid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K09831

sterol 22-desaturase

K09832

sterol 22-desaturase

Genes

ATH:	AT2G28850(CYP710A3) AT2G28860(CYP710A4) AT2G34490(CYP710A2) AT2G34500(CYP710A1)

ALY:	9317081 9317405 9317406

CRB:	17887986 17889424 17889482

CSAT:

104699232 104699233 104700630 104750004 104750006 104753438 104781842 104781843 104786110 104786111 104786814 104789238 104789239 104789240 104792202 104792203

EUS:	EUTSA_v10016547mg EUTSA_v10016552mg

BRP:	103865343 103867449

BNA:	106432759 106447495 125585615 125608302 125609361

BOE:	106339100 106339917 106340751 106341405

RSZ:	108807244 108833237 108852806

THJ:

104814293

CPAP:

110807637

CIT:

102618952

CIC:	CICLE_v10014973mg

PVY:

116141721

MINC:

123192456 123218316

TCC:

18596516

GRA:	105769297 105769298 105772027 105774040

GHI:	107901888 107905877 107905878 107942637 121203644

GAB:	108452825 108452842

DZI:	111280976 111318668

EGR:

104440796

GMX:	100787616 100808148

GSJ:	114377625 114387884

PVU:	PHAVU_006G163800g

VRA:

106776160

VAR:

108340983

VUN:

114188144

CCAJ:

109789198

APRC:

113852140

MTR:

11437817

TPRA:

123898150 123905820

CAM:

101489453

PSAT:

127079474

ADU:

107468177

AIP:

107623676

AHF:	112714308 112782610

LANG:

109328906 109329075 109345270

PCIN:

129286005

FVE:

105350241

RCN:

112166774

PPER:

18778941

PMUM:

103325114

PAVI:

110749808 110773983

PDUL:

117625864

MDM:

103434989

MSYL:

126621236

ZJU:

107413371

MNT:

21404536

CSV:

101221194

CMO:

103484375

BHJ:

120074625

CMAX:

111478743 111496674

CMOS:

111433344 111433531

CPEP:

111782177 111803380

HBR:	110640583 110645097

MESC:

110609904 110621253

POP:

7472572

PEU:

105117943

PALZ:

118050840

JRE:

109021374

CILL:

122275110

QSU:

112009839

QLO:	115980600 115992293

TWL:

119995149

VVI:

100255505

VRI:

117922864

SLY:	100136886(CYP710A11)

SPEN:

107011895

SOT:

102581211

SSTN:

125848721

CANN:

107859112

NTA:	107763057 107771426(SD1)

NSY:

104234654

NTO:

104096813

NAU:

109208410

INI:

109192033

ITR:

116017561

SIND:

105167297 105172499

OEU:	111407278 111412227

EGT:

105951371

SSPL:

121805298 121808148

SMIL:

131013605

APAN:

127254813

HAN:	110911670 110937398

ECAD:

122593276

LSV:

111890946

CCAV:

112504656

DCR:

108199974

CSIN:

114313482

RVL:	131312423 131312424 131312426

BVG:

104883528

SOE:	110778797 110778903

CQI:	110730201 110732522

ATRI:

130814489 130823996

MOF:

131144302

NNU:

104597733

MING:

122060078 122092751

TSS:	122639679 122650136

PSOM:

113329902 113338741 113345304 113355287

OSA:	4325562 4325563 4325564 9267882

DOSA:

Os01t0210900-01(Os01g0210900) Os01t0211000-00(Os01g0211000) Os01t0211200-00(Os01g0211200) Os01t0211600-01(Os01g0211600)

OBR:	102709230 102711741

OGL:	127759857 127760109 127760169 127760847

BDI:

100827562

ATS:	109746138 109757150 109772439

TDC:	119267541 119275444 119292328 119298617 119304413 119338528 119338529 119343557 119344120

TAES:

123060655 123069249 123077753 123094069 123099277 123157569 123162932

TUA:	125507657 125543216

LPER:

127294839 127296147 127296148 127321331

LRD:	124647277 124658988 124663956 124663963 124663967 124664979 124667667 124668114 124695192 124695193

SBI:

8079522

ZMA:

107305676

SITA:

101764868 101770311

SVS:	117837101 117858509

PVIR:

120676345 120705614

PHAI:

112893543

PDA:	103702545 103703021

EGU:	105040643 105048162

MUS:	103995072 103999677 104000847

ZOF:	121989158 121998106 122052204 122056687

DCT:

110110071

PEQ:

110029296

AOF:

109828814

NCOL:

116265070

ATR:

18435199

SMO:	SELMODRAFT_234389 SELMODRAFT_75218(CYP710A21)

PPP:	112277322 112285903

SCE:	YMR015C(ERG5)

SEUB:

DI49_4102

SPAO:

SPAR_M01390

AGO:	AGOS_ABR053C

KLA:	KLLA0_D11242g

KMX:	KLMA_60417(cyp524A1)

LTH:	KLTH0D07832g

VPO:	Kpol_1056p10

ZRO:	ZYRO0E06204g

CGR:	CAGL0M07656g

NCS:	NCAS_0A12700(NCAS0A12700)

NDI:	NDAI_0B01560(NDAI0B01560)

TPF:	TPHA_0I02420(TPHA0I02420)

TBL:	TBLA_0H02650(TBLA0H02650)

TGB:	HG536_0G01250

TDL:	TDEL_0B06510(TDEL0B06510)

KAF:	KAFR_0A02110(KAFR0A02110)

KNG:	KNAG_0M02100(KNAG0M02100)

ZMK:	HG535_0G03560

PPA:	PAS_chr3_0507

DHA:	DEHA2D06182g

PIC:	PICST_73043(ERG5)

PGU:	PGUG_05085

SPAA:

SPAPADRAFT_63544(ERG5)

LEL:	LELG_05188

CAL:	CAALFM_C702840CA(ERG5)

CTP:	CTRG_04983

COT:	CORT_0G04140

CDU:	CD36_72510

CTEN:

CANTEDRAFT_115386

YLI:	YALI0A18062g

CLU:	CLUG_04246

CLUS:

A9F13_04g02805

CAUR:

CJI96_0004850 CJI96_0004910

SLB:	AWJ20_75(ERG5)

PKZ:	C5L36_0B12520

BNN:	FOA43_004793(ERG5)

BBRX:

BRETT_002777(ERG5)

OPA:	HPODL_01958

SLUD:

SCDLUD_004216

NCR:

NCU05278

NTE:	NEUTE1DRAFT116692(NEUTE1DRAFT_116692)

SMP:	SMAC_01710

PAN:	PODANSg6262 PODANSg7995

TTT:	THITE_128412

MTM:	MYCTH_73679

CTHR:

CTHT_0029760

MGR:

MGG_06047

PPEI:

PpBr36_02869

TMN:	UCRPA7_7946

SSCK:

SPSK_00476

FGR:	FGSG_01959 FGSG_03686

FPU:	FPSE_01847 FPSE_09181

FVR:	FVEG_07284 FVEG_08786

FOX:	FOXG_04166 FOXG_09685

NHE:	NECHADRAFT_72313 NECHADRAFT_72652

FFC:	NCS54_01269600 NCS54_01305200

TRE:	TRIREDRAFT_122166 TRIREDRAFT_58689

TRR:	M419DRAFT_141218 M419DRAFT_74177

MAW:

MAC_08636

MAJ:

MAA_01109

CMT:

CCM_02962

PLJ:	VFPFJ_00660 VFPFJ_08344

PTKZ:

JDV02_004750 JDV02_007982(ERG5)

VAL:	VDBG_00192 VDBG_00755

VDA:	VDAG_00367 VDAG_08476

CFJ:	CFIO01_05732 CFIO01_13734

CLUP:

CLUP02_04776 CLUP02_14903

CHIG:

CH63R_04552 CH63R_14243

SAPO:

SAPIO_CDS4161 SAPIO_CDS7005

ELA:	UCREL1_11694

PFY:	PFICI_04403 PFICI_13034

SSL:	SS1G_09832

BFU:	BCIN_07g01120(Bcerg5)

MBE:

MBM_06688

PSCO:

LY89DRAFT_257563 LY89DRAFT_584410

GLZ:	GLAREA_02023 GLAREA_06590

ANI:

AN4042.2

AFM:	AFUA_1G03950

ACT:	ACLA_008020 ACLA_030640

NFI:	NFIA_020740

AOR:	AO090003000253 AO090003000954

ANG:	ANI_1_350014(An01g02810) ANI_1_446094(An11g03230)

AFV:	AFLA_000720 AFLA_001436

ALUC:

AKAW2_20450S(ERG5_1) AKAW2_80298S(ERG5_2)

ACHE:

ACHE_11570S(ERG5)

APUU:

APUU_40308A(ERG5) APUU_60358A

PCS:	Pc16g08330 Pc22g07520

PDP:	PDIP_21270

TMF:	EYB26_008188

TRG:	TRUGW13939_00550 TRUGW13939_08662

CIM:	CIMG_05009

CPW:	CPC735_068810

URE:	UREG_06546

PBL:	PAAG_11073(PAAG_00230)

PBN:	PADG_07898

ABE:

ARB_03814

TVE:

TRV_05093

AJE:	HCAG_06013

BGH:	BDBG_03201

PNO:	SNOG_06055

PTE:

PTT_19871

BZE:	COCCADRAFT_88203

BSC:	COCSADRAFT_221542

BOR:	COCMIDRAFT_93916

AALT:

CC77DRAFT_991604

ZTR:	MYCGRDRAFT_55438(CYP-70)

PFJ:	MYCFIDRAFT_49450

FFU:	CLAFUR5_02386

BCOM:

BAUCODRAFT_489380

NPA:	UCRNP2_2755

TML:	GSTUM_00005882001

SPO:	SPAC19A8.04(erg5)

SOM:	SOMG_03716(erg5)

CNE:

CNF03720

CNB:

CNBF1100

CNG:	CNAG_06644

CGI:	CGB_F1500C

TMS:	TREMEDRAFT_40696

TASA:

A1Q1_00690

TVS:	TRAVEDRAFT_140384

DSQ:	DICSQDRAFT_66936

PCO:	PHACADRAFT_246788

SHS:	STEHIDRAFT_123767

HIR:	HETIRDRAFT_141009(cpm111)

PSQ:	PUNSTDRAFT_59236

ADL:	AURDEDRAFT_109660

FME:	FOMMEDRAFT_106824 FOMMEDRAFT_155371

GTR:	GLOTRDRAFT_53715

RSX:	RhiXN_01858

LBC:	LACBIDRAFT_187166(ERG5)

CCI:	CC1G_01671

ABP:	AGABI1DRAFT66440(AGABI1DRAFT_66440)

ABV:	AGABI2DRAFT189837(AGABI2DRAFT_189837)

PCUB:

JR316_0000711

MRR:	Moror_12729

E1B28_009893

SCM:	SCHCO_02609502(SCHCODRAFT_02609502)

CPUT:

CONPUDRAFT_94730

SLA:	SERLADRAFT_459032

WSE:	WALSEDRAFT_69870

WIC:	J056_001310

UMA:	UMAG_00350

PFP:	PFL1_03644

SGRA:

EX895_000577

MGL:

MGL_0310

MRT:

MRET_0994

MSYM:

MSY001_2710

» show all

Reference

1 [PMID:8543054]

Authors

Kelly SL, Lamb DC, Corran AJ, Baldwin BC, Parks LW, Kelly DE

Title

Purification and reconstitution of activity of Saccharomyces cerevisiae P450 61, a sterol delta 22-desaturase.

Journal

FEBS Lett 377:217-20 (1995)
DOI:10.1016/0014-5793(95)01342-3

Reference

2 [PMID:8635732]

Authors

Skaggs BA, Alexander JF, Pierson CA, Schweitzer KS, Chun KT, Koegel C, Barbuch R, Bard M

Title

Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis.

Journal

Gene 169:105-9 (1996)
DOI:10.1016/0378-1119(95)00770-9

Sequence

[sce:YMR015C]

Reference

3 [PMID:16531502]

Authors

Morikawa T, Mizutani M, Aoki N, Watanabe B, Saga H, Saito S, Oikawa A, Suzuki H, Sakurai N, Shibata D, Wadano A, Sakata K, Ohta D.

Title

Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato.

Journal

Plant Cell 18:1008-22 (2006)
DOI:10.1105/tpc.105.036012