KEGG ENZYME: 1.14.19.41

ENZYME: 1.14.19.41

Entry

EC 1.14.19.41 Enzyme

Name

sterol 22-desaturase;
ERG5 (gene name);
CYP710A (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water

Sysname

ergosta-5,7,24(28)-trien-3beta-ol,NADPH:oxygen oxidoreductase (22,23-dehydrogenating)

Reaction(IUBMB)

ergosta-5,7,24(28)-trien-3beta-ol + NADPH + H+ + O2 = ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADP+ + 2 H2O [RN:R07506]

Reaction(KEGG)

R07506;
(other) R07489 R11097

Substrate

ergosta-5,7,24(28)-trien-3beta-ol [CPD:C15778];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

ergosta-5,7,22,24(28)-tetraen-3beta-ol [CPD:C05440];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Comment

A heme-thiolate protein (P-450). The enzyme, found in yeast and plants, catalyses the introduction of a double bond between the C-22 and C-23 carbons of certain sterols. In yeast the enzyme acts on ergosta-5,7,24(28)-trien-3beta-ol, a step in the biosynthesis of ergosterol. The enzyme from the plant Arabidopsis thaliana acts on sitosterol and 24-epi-campesterol, producing stigmasterol and brassicasterol, respectively.

History

EC 1.14.19.41 created 2015

Pathway

ec00100

Steroid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K09831

sterol 22-desaturase

K09832

sterol 22-desaturase

Genes

ATH:	AT2G28850(CYP710A3) AT2G28860(CYP710A4) AT2G34490(CYP710A2) AT2G34500(CYP710A1)

ALY:	ARALYDRAFT_901838 ARALYDRAFT_902534 ARALYDRAFT_902535

CRB:	17887986 17889424 17889482

CSAT:

104699232 104699233 104700630 104704414 104750004 104750006 104753438 104781842 104781843 104786110 104786111 104786814 104789238 104789239 104789240 104792202 104792203

EUS:	EUTSA_v10016547mg EUTSA_v10016552mg

BRP:	103865343 103867449

BNA:	106396203 106432759 106432788 106447495

BOE:	106339100 106339917 106340751 106341405

RSZ:	108807244 108833237 108852806

THJ:

104814293

CPAP:

110807637

CIT:

102618952

CIC:	CICLE_v10014973mg

TCC:

18596516

GRA:	105769297 105769298 105772027 105774040

GHI:	107901887 107905877 107905878 107942637

GAB:	108452825 108452842

DZI:	111280976 111318668

EGR:

104440796

GMX:	100787616 100808148

GSJ:	114377625 114387884

PVU:	PHAVU_006G163800g

VRA:

VAR:

VUN:

CCAJ:

MTR:	MTR_2g019640

CAM:

101489453

LJA:	Lj6g3v1932820.1(Lj6g3v1932820.1)

ADU:

107468177

AIP:

107623676

LANG:

109328906 109329075 109345270

FVE:

RCN:

PPER:

PMUM:

PAVI:

MDM:

ZJU:

CSV:

CMO:

MCHA:

CMAX:

CMOS:

CPEP:

JCU:

HBR:	110640583 110645097

MESC:

POP:

PEU:

JRE:

QSU:	112004265 112009839 112037010 112037011

VVI:

100255505

SLY:	100136886(SD1)

SPEN:

107011895

SOT:

102581211

CANN:

107859112

NTA:	107763057 107771426(SD1)

NSY:

NTO:

NAU:

INI:

SIND:

OEU:	111407278 111412227

HAN:	110911670 110937398

LSV:

CCAV:

DCR:

BVG:

SOE:	110778797 110778903

CQI:	110730201 110732522

NNU:

104597733

PSOM:

113329902 113338741 113345304 113351226 113355287

OSA:	4325562 4325563 4325564 9267882

DOSA:

Os01t0210900-01(Os01g0210900) Os01t0211000-00(Os01g0211000) Os01t0211200-00(Os01g0211200) Os01t0211600-01(Os01g0211600)

OBR:	102709230 102711741

BDI:

100827562

ATS:	109746138(LOC109746138) 109757150(LOC109757150) 109772439(LOC109772439)

SBI:

8079522

ZMA:

107305676

SITA:

101764868 101770311

PDA:	103702545 103703021

EGU:	105040643 105048162

MUS:	103995072 103999677 104000847

DCT:

PEQ:

AOF:

ATR:

SMO:	SELMODRAFT_234389 SELMODRAFT_75218(CYP710A21)

PPP:	112277322 112285903

VCN:	VOLCADRAFT_68965

MNG:

MNEG_5387

CSL:	COCSUDRAFT_29437

CVR:	CHLNCDRAFT_30281

APRO:

F751_0963

CME:	CYME_CMJ270C CYME_CMJ284C

GSL:	Gasu_47480

SCE:	YMR015C(ERG5)

AGO:	AGOS_ABR053C

KLA:	KLLA0_D11242g

KMX:	KLMA_60417(cyp524A1)

LTH:	KLTH0D07832g

VPO:	Kpol_1056p10

ZRO:	ZYRO0E06204g

CGR:	CAGL0M07656g

NCS:	NCAS_0A12700(NCAS0A12700)

NDI:	NDAI_0B01560(NDAI0B01560)

TPF:	TPHA_0I02420(TPHA0I02420)

TBL:	TBLA_0H02650(TBLA0H02650)

TDL:	TDEL_0B06510(TDEL0B06510)

KAF:	KAFR_0A02110(KAFR0A02110)

PPA:	PAS_chr3_0507

DHA:	DEHA2D06182g

PIC:	PICST_73043(ERG5)

PGU:	PGUG_05085

SPAA:

SPAPADRAFT_63544(ERG5)

LEL:	LELG_05188

CAL:	CAALFM_C702840CA(ERG5)

CTP:	CTRG_04983

COT:	CORT_0G04140

CDU:	CD36_72510

CTEN:

CANTEDRAFT_115386

YLI:	YALI0A18062g

CLU:	CLUG_04246

CLUS:

A9F13_04g02805

CAUR:

CJI97_001893

SLB:	AWJ20_75(ERG5)

PKZ:	C5L36_0B12520

NCR:

NCU05278

NTE:	NEUTE1DRAFT116692(NEUTE1DRAFT_116692)

SMP:	SMAC_01710

PAN:	PODANSg6262 PODANSg7995

TTT:	THITE_128412

MTM:	MYCTH_73679

CTHR:

CTHT_0029760

MGR:

MGG_06047

TMN:	UCRPA7_7946

SSCK:

SPSK_00476

FGR:	FGSG_01959 FGSG_03686

FPU:	FPSE_01847 FPSE_09181

FVR:	FVEG_07284 FVEG_08786

FOX:	FOXG_04166 FOXG_09685

NHE:	NECHADRAFT_72313 NECHADRAFT_72652

TRE:	TRIREDRAFT_122166 TRIREDRAFT_58689

TRR:	M419DRAFT_141218 M419DRAFT_74177

MAW:

MAC_08636

MAJ:

MAA_01109

CMT:

CCM_02962

PLJ:	VFPFJ_00660 VFPFJ_08344

VAL:	VDBG_00192 VDBG_00755

VDA:	VDAG_00367 VDAG_08476

CFJ:	CFIO01_05732 CFIO01_13734

ELA:	UCREL1_11694

PFY:	PFICI_04403 PFICI_13034

SSL:	SS1G_09832

BFU:	BCIN_07g01120(Bcerg5)

MBE:

MBM_06688

PSCO:

LY89DRAFT_257563 LY89DRAFT_584410

GLZ:	GLAREA_02023 GLAREA_06590

ANI:

AN4042.2

AFM:	AFUA_1G03950

AOR:	AO090003000253 AO090003000954

ANG:	ANI_1_350014(An01g02810) ANI_1_446094(An11g03230)

AFV:	AFLA_028640 AFLA_035650

ACT:	ACLA_008020 ACLA_030640

NFI:	NFIA_020740

PCS:	Pc16g08330

PDP:	PDIP_21270

CIM:	CIMG_05009

CPW:	CPC735_068810

PBL:	PAAG_11073(PAAG_00230)

PBN:	PADG_07898

URE:	UREG_06546

ABE:

ARB_03814

TVE:

TRV_05093

AJE:	HCAG_06013

PNO:	SNOG_06055

PTE:

PTT_19871

BZE:	COCCADRAFT_88203

BSC:	COCSADRAFT_221542

BOR:	COCMIDRAFT_93916

AALT:

CC77DRAFT_991604

ZTR:	MYCGRDRAFT_55438(CYP-70)

PFJ:	MYCFIDRAFT_49450

BCOM:

BAUCODRAFT_489380

NPA:	UCRNP2_2755

TML:	GSTUM_00005882001

SPO:	SPAC19A8.04(erg5)

CNE:

CNF03720

CNB:

CNBF1100

CGI:	CGB_F1500C

TMS:	TREMEDRAFT_40696

TVS:	TRAVEDRAFT_140384

DSQ:	DICSQDRAFT_66936

PCO:	PHACADRAFT_246788

SHS:	STEHIDRAFT_123767

HIR:	HETIRDRAFT_141009(cpm111)

PSQ:	PUNSTDRAFT_59236

ADL:	AURDEDRAFT_109660

FME:	FOMMEDRAFT_106824 FOMMEDRAFT_155371

GTR:	GLOTRDRAFT_53715

LBC:	LACBIDRAFT_187166(ERG5)

MPR:	MPER_06200

MRR:	Moror_12729

CCI:	CC1G_01671

SCM:	SCHCODRAFT_13845

ABP:	AGABI1DRAFT66440(AGABI1DRAFT_66440)

ABV:	AGABI2DRAFT189837(AGABI2DRAFT_189837)

CPUT:

CONPUDRAFT_94730

SLA:	SERLADRAFT_459032

WSE:	WALSEDRAFT_69870

WIC:	J056_001310

UMA:	UMAG_00350

PFP:	PFL1_03644

MGL:

MGL_0310

MRT:

MRET_0994

DDI:	DDB_G0269016(CYP524A1)

DPP:	DICPUDRAFT_54169

DFA:	DFA_11768(CYP524A1)

ACAN:

ACA1_073430

TCR:	506945.190

LMA:	LMJF_30_3550

LIF:	LINJ_30_3610

LDO:	LDBPK_303610

LMI:	LMXM_29_3550

LBZ:	LBRM_30_3580

» show all

Reference

1 [PMID:8543054]

Authors

Kelly SL, Lamb DC, Corran AJ, Baldwin BC, Parks LW, Kelly DE

Title

Purification and reconstitution of activity of Saccharomyces cerevisiae P450 61, a sterol delta 22-desaturase.

Journal

FEBS Lett 377:217-20 (1995)
DOI:10.1016/0014-5793(95)01342-3

Reference

2 [PMID:8635732]

Authors

Skaggs BA, Alexander JF, Pierson CA, Schweitzer KS, Chun KT, Koegel C, Barbuch R, Bard M

Title

Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis.

Journal

Gene 169:105-9 (1996)
DOI:10.1016/0378-1119(95)00770-9

Sequence

[sce:YMR015C]

Reference

3 [PMID:16531502]

Authors

Morikawa T, Mizutani M, Aoki N, Watanabe B, Saga H, Saito S, Oikawa A, Suzuki H, Sakurai N, Shibata D, Wadano A, Sakata K, Ohta D

Title

Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato.

Journal

Plant Cell 18:1008-22 (2006)
DOI:10.1105/tpc.105.036012