Entry |
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Name |
sterol 22-desaturase;
ERG5 (gene name);
CYP710A (gene name)
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Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
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Sysname |
ergosta-5,7,24(28)-trien-3beta-ol,NADPH:oxygen oxidoreductase (22,23-dehydrogenating)
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Reaction(IUBMB) |
ergosta-5,7,24(28)-trien-3beta-ol + NADPH + H+ + O2 = ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADP+ + 2 H2O [RN: R07506]
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Reaction(KEGG) |
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Substrate |
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Product |
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Comment |
A heme-thiolate protein (P-450). The enzyme, found in yeast and plants, catalyses the introduction of a double bond between the C-22 and C-23 carbons of certain sterols. In yeast the enzyme acts on ergosta-5,7,24(28)-trien-3beta-ol, a step in the biosynthesis of ergosterol. The enzyme from the plant Arabidopsis thaliana acts on sitosterol and 24-epi-campesterol, producing stigmasterol and brassicasterol, respectively.
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History |
EC 1.14.19.41 created 2015
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Pathway |
ec01110 | Biosynthesis of secondary metabolites |
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Orthology |
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Genes |
» show all
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Reference |
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Authors |
Kelly SL, Lamb DC, Corran AJ, Baldwin BC, Parks LW, Kelly DE |
Title |
Purification and reconstitution of activity of Saccharomyces cerevisiae P450 61, a sterol delta 22-desaturase. |
Journal |
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Reference |
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Authors |
Skaggs BA, Alexander JF, Pierson CA, Schweitzer KS, Chun KT, Koegel C, Barbuch R, Bard M |
Title |
Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis. |
Journal |
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Sequence |
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Reference |
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Authors |
Morikawa T, Mizutani M, Aoki N, Watanabe B, Saga H, Saito S, Oikawa A, Suzuki H, Sakurai N, Shibata D, Wadano A, Sakata K, Ohta D. |
Title |
Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato. |
Journal |
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Sequence |
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Other DBs |
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