KEGG   ENZYME: 1.14.19.41Help
Entry
EC 1.14.19.41               Enzyme                                 

Name
sterol 22-desaturase;
ERG5 (gene name);
CYP710A (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
BRITE hierarchy
Sysname
ergosta-5,7,24(28)-trien-3beta-ol,NADPH:oxygen oxidoreductase (22,23-dehydrogenating)
Reaction(IUBMB)
ergosta-5,7,24(28)-trien-3beta-ol + NADPH + H+ + O2 = ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADP+ + 2 H2O [RN:R07506]
Reaction(KEGG)
R07506;
(other) R07489 R11097
Show
Substrate
ergosta-5,7,24(28)-trien-3beta-ol [CPD:C15778];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
ergosta-5,7,22,24(28)-tetraen-3beta-ol [CPD:C05440];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A heme-thiolate protein (P-450). The enzyme, found in yeast and plants, catalyses the introduction of a double bond between the C-22 and C-23 carbons of certain sterols. In yeast the enzyme acts on ergosta-5,7,24(28)-trien-3beta-ol, a step in the biosynthesis of ergosterol. The enzyme from the plant Arabidopsis thaliana acts on sitosterol and 24-epi-campesterol, producing stigmasterol and brassicasterol, respectively.
History
EC 1.14.19.41 created 2015
Pathway
ec00100  Steroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K09831  sterol 22-desaturase
K09832  sterol 22-desaturase
Genes
ATH: AT2G28850(CYP710A3) AT2G28860(CYP710A4) AT2G34490(CYP710A2) AT2G34500(CYP710A1)
ALY: ARALYDRAFT_901838 ARALYDRAFT_902534 ARALYDRAFT_902535
CRB: 17887986 17889424 17889482
CSAT: 104699232 104699233 104700630 104704414 104750004 104750006 104753438 104781842 104781843 104786110 104786111 104786814 104789238 104789239 104789240 104792202 104792203
EUS: EUTSA_v10016547mg EUTSA_v10016552mg
BRP: 103865343 103867449
BNA: 106396203 106432759 106432788 106447495
BOE: 106339100 106339917 106340751 106341405
RSZ: 108807244 108833237 108852806
THJ: 104814293
CPAP: 110807637
CIT: 102618952
TCC: 18596516
EGR: 104440796
VRA: 106776160
VAR: 108340983
VUN: 114188144
CCAJ: 109789198
CAM: 101489453
LJA: Lj6g3v1932820.1(Lj6g3v1932820.1)
ADU: 107468177
AIP: 107623676
FVE: 105350241
RCN: 112166774
PPER: 18778941
PMUM: 103325114
MDM: 103434989
ZJU: 107413371
CSV: 101221194
CMO: 103484375
MCHA: 111015778
JCU: 105650293
POP: 7472572
PEU: 105117943
JRE: 109021374
VVI: 100255505
SLY: 100136886(SD1)
SPEN: 107011895
SOT: 102581211
CANN: 107859112
NTA: 107763057 107771426(SD1)
NSY: 104234654
NTO: 104096813
NAU: 109208410
INI: 109192033
LSV: 111890946
CCAV: 112504656
DCR: 108199974
BVG: 104883528
NNU: 104597733
DOSA: Os01t0210900-01(Os01g0210900) Os01t0211000-00(Os01g0211000) Os01t0211200-00(Os01g0211200) Os01t0211600-01(Os01g0211600)
BDI: 100827562
ATS: 109746138(LOC109746138) 109757150(LOC109757150) 109772439(LOC109772439)
SBI: 8079522
ZMA: 107305676
DCT: 110110071
PEQ: 110029296
AOF: 109828814
ATR: 18435199
MNG: MNEG_5387
APRO: F751_0963
SCE: YMR015C(ERG5)
KMX: KLMA_60417(cyp524A1)
NCS: NCAS_0A12700(NCAS0A12700)
NDI: NDAI_0B01560(NDAI0B01560)
TPF: TPHA_0I02420(TPHA0I02420)
TBL: TBLA_0H02650(TBLA0H02650)
TDL: TDEL_0B06510(TDEL0B06510)
KAF: KAFR_0A02110(KAFR0A02110)
PIC: PICST_73043(ERG5)
SPAA: SPAPADRAFT_63544(ERG5)
CAL: CAALFM_C702840CA(ERG5)
SLB: AWJ20_75(ERG5)
NCR: NCU05278
NTE: NEUTE1DRAFT116692(NEUTE1DRAFT_116692)
MGR: MGG_06047
SSCK: SPSK_00476
MAW: MAC_08636
MAJ: MAA_01109
CMT: CCM_02962
BFU: BCIN_07g01120(Bcerg5)
MBE: MBM_06688
ANI: AN4042.2
ANG: ANI_1_350014(An01g02810) ANI_1_446094(An11g03230)
PBL: PAAG_11073(PAAG_00230)
ABE: ARB_03814
TVE: TRV_05093
PTE: PTT_19871
ZTR: MYCGRDRAFT_55438(CYP-70)
SPO: SPAC19A8.04(erg5)
CNE: CNF03720
CNB: CNBF1100
HIR: HETIRDRAFT_141009(cpm111)
LBC: LACBIDRAFT_187166(ERG5)
ABP: AGABI1DRAFT66440(AGABI1DRAFT_66440)
ABV: AGABI2DRAFT189837(AGABI2DRAFT_189837)
MGL: MGL_0310
MRT: MRET_0994
DDI: DDB_G0269016(CYP524A1)
DFA: DFA_11768(CYP524A1)
 » show all
Taxonomy
Reference
1  [PMID:8543054]
  Authors
Kelly SL, Lamb DC, Corran AJ, Baldwin BC, Parks LW, Kelly DE
  Title
Purification and reconstitution of activity of Saccharomyces cerevisiae P450 61,  a sterol delta 22-desaturase.
  Journal
FEBS Lett 377:217-20 (1995)
DOI:10.1016/0014-5793(95)01342-3
Reference
2  [PMID:8635732]
  Authors
Skaggs BA, Alexander JF, Pierson CA, Schweitzer KS, Chun KT, Koegel C, Barbuch R, Bard M
  Title
Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis.
  Journal
Gene 169:105-9 (1996)
DOI:10.1016/0378-1119(95)00770-9
  Sequence
[sce:YMR015C]
Reference
3  [PMID:16531502]
  Authors
Morikawa T, Mizutani M, Aoki N, Watanabe B, Saga H, Saito S, Oikawa A, Suzuki H, Sakurai N, Shibata D, Wadano A, Sakata K, Ohta D
  Title
Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato.
  Journal
Plant Cell 18:1008-22 (2006)
DOI:10.1105/tpc.105.036012
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.41
IUBMB Enzyme Nomenclature: 1.14.19.41
ExPASy - ENZYME nomenclature database: 1.14.19.41
BRENDA, the Enzyme Database: 1.14.19.41

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