KEGG   ENZYME: 1.14.19.43
Entry
EC 1.14.19.43               Enzyme                                 
Name
palmitoyl-[glycerolipid] 3-(E)-desaturase;
FAD4
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
1-acyl-2-palmitoyl-[glycerolipid],ferredoxin:oxygen oxidoreductase (3,4-trans -dehydrogenating)
Reaction(IUBMB)
a 1-acyl-2-palmitoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = a 1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
Substrate
1-acyl-2-palmitoyl-[glycerolipid];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
O2 [CPD:C00007];
H+ [CPD:C00080]
Product
1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001]
Comment
The enzyme introduces an unusual trans double bond at carbon 3 of a palmitoyl group attached to the sn-2 position of glycerolipids. The enzyme from the plant Arabidopsis thaliana is specific for palmitate in phosphatidylglycerol. The enzyme from tobacco can also accept oleate and alpha-linolenate if present at the sn-2 position of phosphatidylglycerol [1].
History
EC 1.14.19.43 created 2015
Orthology
K20417  palmitoyl-[glycerolipid] 3-(E)-desaturase
Genes
ATHAT1G62190 AT2G22890 AT4G27030(FADA)
ALY9303600 9316535 9322552
CRB17880011 17889867 17894444
CSAT104702825 104713733 104714552 104717530 104722223 104730684 104751928 109125040
EUSEUTSA_v10025879mg EUTSA_v10026787mg EUTSA_v10027264mg
BRP103834822 103835525 103861670 103866795
BNA106373945 106403010 106405551 106410515 106412662 106412663 106440906 106444859 106445558 111211564
BOE106303863 106308091 106310396 106310567 106321690
RSZ108820967 108821631 108822357 108822358 108826401 108852625 108854170
THJ104809192
CPAP110810962
CIT102608519
CICCICLE_v10009005mg
PVY116105927 116107733
MINC123201614
TCC18593565
GRA105780632
GHI107916746 107933433
GAB108480843
DZI111276443
EGR104448334
GMX100793638 100795628
GSJ114394954 114424071
PVUPHAVU_006G040800g
VRA106775460
VAR108342269
VUN114187574
CCAJ109805209
APRC113855030
MTRMTR_3g051220
CAM101506527
LJALj0g3v0362749.1(Lj0g3v0362749.1) Lj6g3v0920100.1(Lj6g3v0920100.1) Lj6g3v0920210.1(Lj6g3v0920210.1)
ADU107479953
AIP107616614
AHF112720289 112789827
LANG109337761 109347611
FVE101296849
RCN112165277
PPER18779734
PMUM103326516
PAVI110758161
PDUL117624771 117625430
MDM103434694 103448508
PXB103927610 103927630 103967035
ZJU107405836 107405839 107431800
MNT21400802
CSV101217074
CMO103501595
BHJ120091830
MCHA111019014
CMAX111476701
CMOS111447974
CPEP111779906
RCU8282921
JCU105632931
HBR110662372
MESC110601667
POP18095957 18103433
PEU105136537 105136540
PALZ118027850
JRE108983937
QSU112012923 112012931 112019947
QLO115956364 115956367 115957374 115957450 115957451
TWL119990057
VVI100257229
VRI117909006
SLY101252202
SPEN107024217
SOT102586585
SSTN125876475
CANN107856304
NTA107760919 107788973
NSY104243685
NTO104097800
NAU109228991
INI109190479
ITR116022715
SIND105166649 105175146 105176767 105176768
OEU111397714 111403959
EGT105948841 105961860 105961861
SSPL121755979 121786768
HAN118492206 118492410
LSV111882811 111897379 111897388
CCAV112514210 112514211 112514212
DCR108211866
CSIN114297295
BVG104891650
SOE110776949 110782171
CQI110720983 110723537 110723571 110723814
NNU104610901
MING122059443 122060224
TSS122641480
PSOM113287904 113304756
NCOL116264693
OSA107277927
DOSAOs08t0187900-00(Os08g0187900)
OBR121055231
BDI100826352
ATS109734181
TDC119315175 119324347
TAES123132194 123136224 123143728
TUA125513953 125513973
ZMA103641109
SITA101754789 101755587
SVS117860181 117860572 117860774
PVIR120677787 120711630
PHAI112898030
PDA103720930
EGU105035002
MUS104000016
AOF109821617
ATR18448014
SMOSELMODRAFT_77032
PPP112290313
CRECHLRE_16g673001v5
MNGMNEG_11576
CSLCOCSUDRAFT_11426
CVRCHLNCDRAFT_137562
APROF751_3272
OTAOT_ostta09g03220
BPGBathy10g01200
MISMICPUN_58724
MPPMICPUCDRAFT_58905
GSLGasu_21010 Gasu_28340
FCYFRACYDRAFT_177929
NGDNGA_0194500
AAFAURANDRAFT_67743 AURANDRAFT_67953
EHXEMIHUDRAFT_115981 EMIHUDRAFT_453290
GTTGUITHDRAFT_76752
GLOGlov_2049
 » show all
Reference
1  [PMID:17158889]
  Authors
Fritz M, Lokstein H, Hackenberg D, Welti R, Roth M, Zahringer U, Fulda M, Hellmeyer W, Ott C, Wolter FP, Heinz E
  Title
Channeling of eukaryotic diacylglycerol into the biosynthesis of plastidial phosphatidylglycerol.
  Journal
J Biol Chem 282:4613-25 (2007)
DOI:10.1074/jbc.M606295200
Reference
2  [PMID:19682287]
  Authors
Gao J, Ajjawi I, Manoli A, Sawin A, Xu C, Froehlich JE, Last RL, Benning C
  Title
FATTY ACID DESATURASE4 of Arabidopsis encodes a protein distinct from characterized fatty acid desaturases.
  Journal
Plant J 60:832-9 (2009)
DOI:10.1111/j.1365-313X.2009.04001.x
  Sequence
[ath:AT4G27030]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.43
IUBMB Enzyme Nomenclature: 1.14.19.43
ExPASy - ENZYME nomenclature database: 1.14.19.43
BRENDA, the Enzyme Database: 1.14.19.43

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