KEGG ENZYME: 1.14.19.44

ENZYME: 1.14.19.44

Entry

EC 1.14.19.44               Enzyme

Name

acyl-CoA (8-3)-desaturase;
FADS1 (gene name);
acyl-CoA 5-desaturase (methylene-interrupted)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water

Sysname

Delta8-acyl-CoA,ferrocytochrome b5:oxygen oxidoreductase (5,6-cis-dehydrogenating)

Reaction(IUBMB)

(1) (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = arachidonoyl-CoA + 2 ferricytochrome b5 + 2 H2O [RN:R11059];
(2) (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-CoA + 2 ferricytochrome b5 + 2 H2O [RN:R11060]

Reaction(KEGG)

R11059 R11060;
(other) R12169

Substrate

(8Z,11Z,14Z)-icosa-8,11,14-trienoyl-CoA [CPD:C03595];
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080];
(8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-CoA [CPD:C16164]

Product

arachidonoyl-CoA [CPD:C02249];
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001];
(5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-CoA [CPD:C16165]

Comment

The enzyme introduces a cis double bond at carbon 5 of acyl-CoAs that contain a double bond at position 8. The enzymes from algae, mosses, mammals and the protozoan Leishmania major catalyse the desaturation of dihomo-gamma-linoleate [(8Z,11Z,14Z)-icosa-8,11,14-trienoate] and (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoate to generate arachidonate and (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoate, respectively. The enzyme contains a cytochrome b5 domain that acts as the direct electron donor to the desaturase active site and does not require an external cytochrome. cf. EC 1.14.19.37, acyl-CoA 5-desaturase.

History

EC 1.14.19.44 created 2015

Pathway

ec01040

Biosynthesis of unsaturated fatty acids

Orthology

K10224

acyl-CoA (8-3)-desaturase (Delta-5 desaturase)

Genes

HSA:	3992(FADS1)

PTR:	451243(FADS1)

PPS:	100981162(FADS1)

GGO:	101136185(FADS1)

PON:	100436159(FADS1)

NLE:	100596120(FADS1)

HMH:	116468867(FADS1)

MCC:	722343(FADS1)

MCF:	101864778(FADS1)

MTHB:

126935470

MNI:	105469425(FADS1)

CSAB:

103234224(FADS1)

CATY:

105577579(FADS1)

PANU:

100126707(FADS1)

TGE:	112606126(FADS1)

MLEU:

105549021

RRO:	104679375(FADS1)

RBB:	108533599(FADS1)

TFN:	117080259(FADS1)

PTEH:

111524317(FADS1)

CANG:

105512288(FADS1)

CJC:	100389579(FADS1)

SBQ:	101052172(FADS1)

CIMI:

108286222(FADS1)

MMUR:

105878618(FADS1)

LCAT:

123641269(FADS1)

PCOQ:

105825052(FADS1)

OGA:	100954652(FADS1)

MMU:	76267(Fads1)

MCAL:

110285897(Fads1)

MPAH:

110322451(Fads1)

RNO:	84575(Fads1)

MCOC:

116103544(Fads1)

ANU:	117707570(Fads1)

MUN:	110548091(Fads1)

CGE:

100773095

MAUA:

101844332(Fads1)

PROB:

127228839(Fads1)

PLEU:

114703435(Fads1)

MORG:

121453877(Fads1)

MFOT:

126498525

AAMP:

119813303(Fads1)

NGI:	103729124(Fads1)

HGL:	101698014(Fads1)

CPOC:

100723348(Fads1)

CCAN:

109685566(Fads1)

DORD:

105995514(Fads1)

DSP:	122104887(Fads1)

PLOP:

125362341(Fads1)

NCAR:

124958643

OCU:

100342403

OPI:	101524219(FADS1)

TUP:	102500595(FADS1)

GVR:	103604811(FADS1)

CFA:	483793(FADS1)

CLUD:

112659419(FADS1)

VVP:	112911088(FADS1)

VLG:	121472259(FADS1)

NPO:	129500987(FADS1)

AML:	100472955(FADS1)

UMR:	103678230(FADS1)

UAH:	113246984(FADS1)

UAR:	123785000(FADS1)

ELK:

111149587

LLV:

125078519

MPUF:

101676081(FADS1)

NVS:	122912991(FADS1)

ORO:	101364825(FADS1)

EJU:	114220205(FADS1)

ZCA:	113914860(FADS1)

MLX:	118015924(FADS1)

NSU:	110576324(FADS1)

LWW:	102739160(FADS1)

FCA:	101083966(FADS1)

PYU:	121043898(FADS1)

PCOO:

112851878

PBG:	122483865(FADS1)

LRUF:

124505586

PTG:	102968608(FADS1)

PPAD:

109246555(FADS1)

PUC:

125911233

AJU:

106986968

HHV:	120246150(FADS1)

BTA:	533107(FADS1)

BOM:	102276098(FADS1)

BIU:	109554369(FADS1)

BBUB:

102405648(FADS1)

BBIS:

104998703(FADS1)

CHX:	102178247(FADS1)

OAS:	101109049(FADS1)

BTAX:

128069158(FADS1)

ODA:	120874548(FADS1)

CCAD:

122431165(FADS1)

MBEZ:

129548883(FADS1)

SSC:	444995(FADS1)

CFR:	102506763(FADS1)

CBAI:

105070110(FADS1)

CDK:	105094872(FADS1)

VPC:	102543424(FADS1)

BACU:

103008410(FADS1)

LVE:	103082696(FADS1)

OOR:	101275971(FADS1)

DLE:	111183884(FADS1)

PCAD:

102988776(FADS1)

PSIU:

116757880(FADS1)

NASI:

112402054(FADS1)

ECB:	102150261(FADS1)

EPZ:	103543089(FADS1)

EAI:	106847567(FADS1)

MYB:	102261592(FADS1)

MYD:	102773181(FADS1)

MMYO:

118649002(FADS1)

MNA:	107527686(FADS1)

PKL:	118707199(FADS1)

EFUS:

103303329(FADS1)

HAI:	109390020(FADS1)

DRO:	112317234(FADS1)

SHON:

118996919(FADS1)

AJM:	119043682(FADS1)

PDIC:

114499452(FADS1)

PHAS:

123829519(FADS1)

MMF:	118627540(FADS1)

RFQ:	117030354(FADS1)

PALE:

102894313(FADS1)

PGIG:

120600651(FADS1)

PVP:	105297785(FADS1)

RAY:	107501590(FADS1)

MJV:	108392394(FADS1)

TOD:	119231397 119250072(FADS1)

SARA:

101540344(FADS1)

LAV:

100677495

TMU:

101347278

ETF:	101661682(FADS1)

DNM:	101417194(FADS1)

MDO:	100028492(FADS1)

SHR:	100922163(FADS1)

AFZ:

127541864

PCW:	110195308(FADS1)

OAA:	103171425(FADS1)

GGA:	423119(FADS1L2) 423120(FADS1) 428851(FADS1L1)

PCOC:

116239003 116239007 116239016

MGP:	100549516 100549671 100549825(FADS1)

CJO:	107314410 107314438 107314472

TPAI:

128085508 128086867(FADS1)

LMUT:

125694681 125695384 125695385

NMEL:

110400732 110400736 110401255(FADS1)

APLA:

101789732 101799007(FADS1)

ACYG:

106033871 106044856 106044871

CATA:

118249736 118250732 118250781

AFUL:

116489595 116489696(FADS1) 116490009

TGU:	100225746(FADS1) 100228631 115495653

LSR:	110484927(FADS1) 110485021 110485022

SCAN:

103822124(FADS1) 103822125 103822228

PMOA:

120499563 120499565(FADS1) 120499600

OTC:	121339155(FADS1) 121339445 121339494

PRUF:

121354751(FADS1) 121354752 121354755

GFR:	102031677 102040395(FADS1) 102040846

FAB:	101809325 101809791(FADS1) 101813351

OMA:	130253517 130253520 130253580

PHI:	102101859 102112391(FADS1) 102112775

PMAJ:

107205341 107205403 107205404

CCAE:

111930634 111930636 111930637

CCW:	104697619 104697620 104697621

CBRC:

103614699 103614730(FADS1) 103614731

ETL:	114066084 114066085 114066089

ZAB:	102066550 102069423 102069595(FADS1)

ACHL:

103805394 103805395 103810955

SVG:	106854655 106854716 106854757(FADS1)

MMEA:

130580092 130580094 130580095

HRT:	120753727 120753733 120753741

FPG:	101910435 101912001(FADS1) 106112851

FCH:	102049758(FADS1) 106631119

CLV:	102094615 102098315(FADS1) 102098790

EGZ:	104124680 104124682 104124699

NNI:	104009395 104009483(FADS1) 104009484

PCRI:

104023942 104036418 104037506

PLET:

104619104 104627919 104627920

EHS:	104502732 104504142 104505341

PCAO:

104045549 104048901 104051707(FADS1)

ACUN:

113481253 113481341 113481355(FADS1)

TALA:

104363221 104368248 104368249(FADS1)

PADL:

103925784 103925826 103925828(FADS1)

AFOR:

103905063 103905173 103907211(FADS1)

ACHC:

115351609 115352165 115352167

HALD:

104313152 104313153 104322733

HLE:	104829426(FADS1) 104829427 104829570

AGEN:

126047207 126047208 126047316

GCL:	127016483 127016609 127016776

CCRI:

104162789 104162790 104166166

CSTI:

104554377 104554378 104562342

CMAC:

104473140 104477798 104477799

MUI:	104538638 104538639 104538640

BREG:

104637184 104637185 104637186

FGA:	104069286 104070764

GSTE:

104253491 104253492 104261229

LDI:	104349394 104351594 104351595

MNB:	103769636 103781635 103781636

OHA:	104339400 104339408(FADS1)

NNT:	104399813 104412620

SHAB:

115606142 115606143 115606144 115607758

DPUB:

104304412 104304440 104304441(FADS1)

PGUU:

104463039 104471831 104471841

ACAR:

104525757 104525765 104525775

CPEA:

104397472 104397492 104397493(FADS1)

AVIT:

104267243 104269712 104274326

CVF:	104287519(FADS1) 104287521 104287566

CUCA:

104062395 104062401(FADS1) 104062546

TEO:	104374175 104382106 104382107

BRHI:

104491024 104492898 104499051

AAM:	106490039 106490040 106490045(FADS1)

AROW:

112978023(FADS1) 112978027 112978037

NPD:	112948005(FADS1) 112948043 112948044

TGT:	104570057 104571492 104571495

DNE:	112991679 112991686 112991696(FADS1)

SCAM:

104139316 104139318

ASN:	102378297 102382011 102382251 102382503 102382756 102386118 102387601 102388109

AMJ:	102563927 102564163 102564392 102564994 102565222(FADS1) 102574216 106737005

CPOO:

109318130 109318222

GGN:

109298190

PSS:	102460779 102461025

CMY:	102932384 102932824 102933055

CCAY:

125639126 125639127 125639129

DCC:	119856947 119857371 119857443

CPIC:

101936861(FADS1) 101937159

TST:	117876524 117877424

CABI:

116827619 116828643 116830901(FADS1)

MRV:	120403578 120403579 120403581 120403991

ACS:	100551618(fads1) 100555351 100555547 100555740 100555937

PVT:	110086171 110086173 110086190 110086192(FADS1) 110086194 110086197

SUND:

121926305 121930479 121930494 121930500 121930504

PBI:	103050792(FADS1) 103053493 103065518

PMUR:

107287991 107291623 107291625 107291626 107291627 107291628 107291629 107291630 107295117

CTIG:

120312436 120312437 120312438 120312441 120312442 120312443 120312444 120312445 120312447 120312448 120312449

TSR:	106538435(FADS1) 106539606 106554505

PGUT:

117667020 117667022 117667025 117667027 117667030

APRI:

131184355 131184372 131184402 131184417 131184426 131184433 131185706 131185771

PTEX:

113440356 113449138 113449140 113449142

NSS:	113421941 113421942 113421943 113424550

VKO:	123021210 123021212 123021213 123021347 123021348

PMUA:

114584095 114584100 114600543 114600551 114600556 114600565

PRAF:

128417621 128417631 128417642 128417672 128417692

ZVI:	118089033 118089041 118089045 118089057 118089062

HCG:	128324944 128334826 128338638 128339229 128339344 128339393 128340181 128340182 128350825

GJA:	107108850 107108852(FADS1) 107121912 107121913 107121914

STOW:

125426611 125426612 125427059 125427338 125427467 125427539

EMC:	129324105 129324107 129324175 129324182 129324322 129324937 129325027

XLA:	108713836(fads1.L) 108715199(fads1.S)

XTR:	100489232(fads1)

NPR:	108794178(FADS1)

RTEM:

120917259(FADS1)

BBUF:

120981188(FADS1)

BGAR:

122920198(FADS1)

MUO:	115468973(FADS1)

GSH:	117352173(FADS1)

AANG:

118215203

LOC:	102691516(fads1)

PSPA:

121330344

PSEX:

120535962

LCM:	102350226(FADS1)

CMK:

103174860

RTP:

109937249

CPLA:

122557874

LERI:

129705772

AJC:

117125539

ATD:

109598043

LGI:	LOTGIDRAFT_170234

PCAN:

112557826

GTT:	GUITHDRAFT_104142

» show all

Reference

1 [PMID:10601301]

Authors

Cho HP, Nakamura M, Clarke SD

Title

Cloning, expression, and fatty acid regulation of the human delta-5 desaturase.

Journal

J Biol Chem 274:37335-9 (1999)
DOI:10.1074/jbc.274.52.37335

Sequence

[hsa:3992]

Reference

2 [PMID:10769175]

Authors

Leonard AE, Kelder B, Bobik EG, Chuang LT, Parker-Barnes JM, Thurmond JM, Kroeger PE, Kopchick JJ, Huang YS, Mukerji P

Title

cDNA cloning and characterization of human Delta5-desaturase involved in the biosynthesis of arachidonic acid.

Journal

Biochem J 347 Pt 3:719-24 (2000)

Sequence

[hsa:3992]

Reference

3 [PMID:16403015]

Authors

Tripodi KE, Buttigliero LV, Altabe SG, Uttaro AD

Title

Functional characterization of front-end desaturases from trypanosomatids depicts the first polyunsaturated fatty acid biosynthetic pathway from a parasitic protozoan.

Journal

FEBS J 273:271-80 (2006)
DOI:10.1111/j.1742-4658.2005.05049.x

Reference

4 [PMID:21193673]

Authors

Tavares S, Grotkjaer T, Obsen T, Haslam RP, Napier JA, Gunnarsson N

Title

Metabolic engineering of Saccharomyces cerevisiae for production of Eicosapentaenoic Acid, using a novel {Delta}5-Desaturase from Paramecium tetraurelia.

Journal

Appl Environ Microbiol 77:1854-61 (2011)
DOI:10.1128/AEM.01935-10

Other DBs

ExplorEnz - The Enzyme Database:

1.14.19.44

IUBMB Enzyme Nomenclature:

1.14.19.44

ExPASy - ENZYME nomenclature database:

1.14.19.44

BRENDA, the Enzyme Database:

1.14.19.44

DBGET integrated database retrieval system