sn-1 linoleoyl-lipid 6-desaturase;
desD (gene name)
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
1-linoleoyl-2-acyl-[glycerolipid],ferredoxin:oxygen oxidoreductase (6,7-cis-dehydrogenating)
a 1-linoleoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = a 1-gamma-linolenoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
reduced ferredoxin [iron-sulfur] cluster [CPD:
oxidized ferredoxin [iron-sulfur] cluster [CPD:
The enzyme, characterized from cyanobacteria, introduces a cis double bond at carbon 6 of linoleoyl groups (18:2) attached to the sn-1 position of glycerolipids. The enzyme is a front-end desaturase, introducing the new double bond between a pre-existing double bond and the carboxyl-end of the fatty acid. It is nonspecific with respect to the polar head group of the glycerolipid.
EC 220.127.116.11 created 2015
Higashi S, Murata N
An in Vivo Study of Substrate Specificities of Acyl-Lipid Desaturases and Acyltransferases in Lipid Synthesis in Synechocystis PCC6803.
Reddy AS, Thomas TL
Expression of a cyanobacterial delta 6-desaturase gene results in gamma-linolenic acid production in transgenic plants.
Kurdrid P, Subudhi S, Hongsthong A, Ruengjitchatchawalya M, Tanticharoen M
Functional expression of Spirulina-Delta6 desaturase gene in yeast, Saccharomyces cerevisiae.