KEGG   ENZYME: 1.14.19.47
Entry
EC 1.14.19.47               Enzyme                                 

Name
acyl-lipid (9-3)-desaturase;
DES6 (gene name);
acyl-lipid 6-desaturase;
acyl-lipid Delta6-desaturase;
Delta6-desaturase (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
Delta9 acyl-[glycerolipid],ferrocytochrome b5:oxygen oxidoreductase (6,7-cis-dehydrogenating)
Reaction(IUBMB)
(1) an alpha-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a stearidonoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O;
(2) a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a gamma-linolenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
Substrate
alpha-linolenoyl-[glycerolipid] [CPD:C21531];
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080];
linoleoyl-[glycerolipid] [CPD:C21529]
Product
stearidonoyl-[glycerolipid];
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001];
gamma-linolenoyl-[glycerolipid]
Comment
The enzyme, characterized from the moss Physcomitrella patens and the plant Borago officinalis (borage), introduces a cis double bond at carbon 6 of several acyl-lipids that contain an existing Delta9 cis double bond. The enzyme contains a cytochrome b5 domain that acts as the electron donor for the active site of the desaturase.
History
EC 1.14.19.47 created 2015
Orthology
K21737  acyl-lipid Delta6-acetylenase / acyl-lipid (9-3)-desaturase
Genes
BFO: 118409704 118409836
BBEL: 109462408 109469769
CIN: 100181774
SCLV: 120339347
LOA: LOAG_10800
NAI: NECAME_05225
LAK: 106165720 106175792 106175795
NVE: 5520800
AMIL: 114962826
PDAM: 113673085
SPIS: 111333863
THJ: 104803726
CPAP: 110822096
CIT: 102624404
PVY: 116143292
TCC: 18588068
EGR: 104436303
GMX: 100775880(SLD1.4) 100776476(SLD1.5)
VRA: 106765081
VAR: 108336250
VUN: 114195260
CCAJ: 109795310
APRC: 113846413
CAM: 101502873
LJA: Lj0g3v0112719.1(Lj0g3v0112719.1) Lj5g3v0405030.1(Lj5g3v0405030.1)
FVE: 101310770
RCN: 112173700
PPER: 18789224
PMUM: 103320564
PAVI: 110764665
PDUL: 117616024
MDM: 103440767
PXB: 103952139
ZJU: 107420047
MNT: 21402436
RCU: 8259572
JCU: 105638142
HBR: 110633287
POP: 7462256
PEU: 105122191
PALZ: 118030785
JRE: 109021249
QLO: 115970363
TWL: 120005399
VVI: 100259521
VRI: 117913126
SLY: 101257882
SPEN: 107028997
SOT: 102599871
NTA: 107768418 107798633(DXDES)
NTO: 104121325
NAU: 109217138
EGT: 105977192
HAN: 110941481
ECAD: 122579280
LSV: 111906061
CCAV: 112504110
BVG: 104886626
NCOL: 116267278
OSA: 4346776
DOSA: Os09t0338500-01(Os09g0338500)
OBR: 102714763
BDI: 100826592
ATS: 109751130
PHAI: 112882473
ATR: 18436755
SMIN: v1.2.012127.t1(symbB.v1.2.012127.t1)
PTI: PHATRDRAFT_29488(PTD6)
FCY: FRACYDRAFT_228533(FAD2)
TPS: THAPSDRAFT_41113(DES3)
SPAR: SPRG_06919
 » show all
Reference
1  [PMID:9108131]
  Authors
Sayanova O, Smith MA, Lapinskas P, Stobart AK, Dobson G, Christie WW, Shewry PR, Napier JA
  Title
Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of delta6-desaturated fatty acids in transgenic tobacco.
  Journal
Proc Natl Acad Sci U S A 94:4211-6 (1997)
DOI:10.1073/pnas.94.8.4211
  Sequence
Reference
2  [PMID:9744093]
  Authors
Girke T, Schmidt H, Zahringer U, Reski R, Heinz E
  Title
Identification of a novel delta 6-acyl-group desaturase by targeted gene disruption in Physcomitrella patens.
  Journal
Plant J 15:39-48 (1998)
DOI:10.1046/j.1365-313X.1998.00178.x
  Sequence
[ppp:112282380]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.47
IUBMB Enzyme Nomenclature: 1.14.19.47
ExPASy - ENZYME nomenclature database: 1.14.19.47
BRENDA, the Enzyme Database: 1.14.19.47

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