KEGG   ENZYME: 1.14.19.48Help
Entry
EC 1.14.19.48               Enzyme                                 

Name
tert-amyl alcohol desaturase;
mdpJK (gene names)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
BRITE hierarchy
Sysname
tert-amyl alcohol,NADPH:oxygen oxidoreductase (1,2-dehydrogenating)
Reaction(IUBMB)
tert-amyl alcohol + NADPH + H+ + O2 = isoprenyl alcohol + NADP+ + 2 H2O [RN:R11477]
Reaction(KEGG)
Substrate
tert-amyl alcohol [CPD:C21401];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
isoprenyl alcohol [CPD:C21402];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Aquincola tertiaricarbonis, is a Rieske nonheme mononuclear iron oxygenase. It can also act, with lower efficiency, on butan-2-ol, converting it to but-1-en-3-ol. Depending on the substrate, the enzyme also catalyses EC 1.14.13.229, tert-butanol monooxygenase.
History
EC 1.14.19.48 created 2016
Orthology
K21323  tert-butyl alcohol monooxygenase / tert-amyl alcohol desaturase
Genes
MPT: Mpe_B0555
Taxonomy
Reference
1  [PMID:22752178]
  Authors
Schafer F, Schuster J, Wurz B, Hartig C, Harms H, Muller RH, Rohwerder T
  Title
Synthesis of short-chain diols and unsaturated alcohols from secondary alcohol substrates by the Rieske nonheme mononuclear iron oxygenase MdpJ.
  Journal
Appl Environ Microbiol 78:6280-4 (2012)
DOI:10.1128/AEM.01434-12
Reference
2  [PMID:22194447]
  Authors
Schuster J, Schafer F, Hubler N, Brandt A, Rosell M, Hartig C, Harms H, Muller RH, Rohwerder T
  Title
Bacterial degradation of tert-amyl alcohol proceeds via hemiterpene 2-methyl-3-buten-2-ol by employing the tertiary alcohol desaturase function of the Rieske nonheme mononuclear iron oxygenase MdpJ.
  Journal
J Bacteriol 194:972-81 (2012)
DOI:10.1128/JB.06384-11
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.48
IUBMB Enzyme Nomenclature: 1.14.19.48
ExPASy - ENZYME nomenclature database: 1.14.19.48
BRENDA, the Enzyme Database: 1.14.19.48

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