Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
BRITE hierarchy

4'-O-methylnorbelladine,NADPH---hemoprotein reductase:oxygen oxidoreductase (noroxomaritidine-forming)

(1) 4'-O-methylnorbelladine + [reduced NADPH---hemoprotein reductase] + O2 = (4aR,10bS)-noroxomaritidine + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R11483];
(2) 4'-O-methylnorbelladine + [reduced NADPH---hemoprotein reductase] + O2 = (4aS,10bR)-noroxomaritidine + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R11482]

R11482 R11483
Reaction

4'-O-methylnorbelladine [CPD:C16702];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]

A P-450 (heme-thiolate) enzyme. The enzyme, characterized from Narcissus pseudonarcissus (daffodil), forms the two enantiomers of the Amaryllidacea alkaloid noroxomaritidine by catalysing intramolecular oxidative para-para' phenol coupling. The oxidation involves molecular oxygen without its incorporation into the product.

EC 1.14.19.50 created 2016

1  [PMID:26941773]

Kilgore MB, Augustin MM, May GD, Crow JA, Kutchan TM

CYP96T1 of Narcissus sp. aff. pseudonarcissus Catalyzes Formation of the Para-Para' C-C Phenol Couple in the Amaryllidaceae Alkaloids.

Front Plant Sci 7:225 (2016)
DOI:10.3389/fpls.2016.00225

[ag:AMO65741]