Entry |
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Name |
noroxomaritidine synthase;
CYP96T1 (gene name)
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Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
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Sysname |
4'-O-methylnorbelladine,NADPH---hemoprotein reductase:oxygen oxidoreductase (noroxomaritidine-forming)
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Reaction(IUBMB) |
(1) 4'-O-methylnorbelladine + [reduced NADPH---hemoprotein reductase] + O2 = (4aR,10bS)-noroxomaritidine + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN: R11483];
(2) 4'-O-methylnorbelladine + [reduced NADPH---hemoprotein reductase] + O2 = (4aS,10bR)-noroxomaritidine + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN: R11482]
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Reaction(KEGG) |
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Substrate |
4'-O-methylnorbelladine [CPD: C16702];
[reduced NADPH---hemoprotein reductase] [CPD: C03024];
O2 [CPD: C00007]
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Product |
(4aR,10bS)-noroxomaritidine [CPD: C21406];
[oxidized NADPH---hemoprotein reductase] [CPD: C03161];
H2O [CPD: C00001];
(4aS,10bR)-noroxomaritidine [CPD: C21405]
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Comment |
A P-450 (heme-thiolate) enzyme. The enzyme, characterized from Narcissus pseudonarcissus (daffodil), forms the two enantiomers of the Amaryllidacea alkaloid noroxomaritidine by catalysing intramolecular oxidative para-para' phenol coupling. The oxidation involves molecular oxygen without its incorporation into the product.
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History |
EC 1.14.19.50 created 2016
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Pathway |
ec00950 | Isoquinoline alkaloid biosynthesis |
ec01110 | Biosynthesis of secondary metabolites |
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Orthology |
K21208 | noroxomaritidine synthase |
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Reference |
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Authors |
Kilgore MB, Augustin MM, May GD, Crow JA, Kutchan TM |
Title |
CYP96T1 of Narcissus sp. aff. pseudonarcissus Catalyzes Formation of the Para-Para' C-C Phenol Couple in the Amaryllidaceae Alkaloids. |
Journal |
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Sequence |
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Other DBs |
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