KEGG   ENZYME: 1.14.19.56Help
Entry
EC 1.14.19.56               Enzyme                                 

Name
1H-pyrrole-2-carbonyl-[peptidyl-carrier protein] chlorinase;
pltA (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
BRITE hierarchy
Sysname
1H-pyrrole-2-carbonyl-[peptidyl-carrier protein]:FADH2 oxidoreductase (chlorinating)
Reaction(IUBMB)
1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + 2 FADH2 + 2 chloride + 2 O2 = 4,5-dichloro-1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + 2 FAD + 4 H2O (overall reaction) [RN:R12023];
(1a) 1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + FADH2 + chloride + O2 = 5-chloro-1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + FAD + 2 H2O [RN:R12021];
(1b) 5-chloro-1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + FADH2 + chloride + O2 = 4,5-dichloro-1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + FAD + H2O [RN:R12022]
Reaction(KEGG)
Substrate
1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein];
FADH2 [CPD:C01352];
chloride [CPD:C00698];
O2 [CPD:C00007];
5-chloro-1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein]
Product
4,5-dichloro-1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein];
FAD [CPD:C00016];
H2O [CPD:C00001];
5-chloro-1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein]
Comment
The enzyme, characterized from the bacterium Pseudomonas protegens Pf-5, is a flavin-dependent chlorinase that participates in the biosynthesis of the antibacterial and antifungal compound pyoluteorin.
History
EC 1.14.19.56 created 2018
Orthology
K22712  1H-pyrrole-2-carbonyl-[peptidyl-carrier protein] chlorinase
Genes
PAG: PLES_25811(pltA)
PAF: PAM18_2386(pltA)
PAEL: T223_13190
PFL: PFL_2787(pltA)
PPRC: PFLCHA0_c28450(pltA)
PPRO: PPC_2832(pltA)
Taxonomy
Reference
1  [PMID:10094695]
  Authors
Nowak-Thompson B, Chaney N, Wing JS, Gould SJ, Loper JE
  Title
Characterization of the pyoluteorin biosynthetic gene cluster of Pseudomonas fluorescens Pf-5.
  Journal
J Bacteriol 181:2166-74 (1999)
  Sequence
[pfl:PFL_2787]
Reference
2  [PMID:16162666]
  Authors
Dorrestein PC, Yeh E, Garneau-Tsodikova S, Kelleher NL, Walsh CT
  Title
Dichlorination of a pyrrolyl-S-carrier protein by FADH2-dependent halogenase PltA during pyoluteorin biosynthesis.
  Journal
Proc Natl Acad Sci U S A 102:13843-8 (2005)
DOI:10.1073/pnas.0506964102
Reference
3  [PMID:26416533]
  Authors
Pang AH, Garneau-Tsodikova S, Tsodikov OV
  Title
Crystal structure of halogenase PltA from the pyoluteorin biosynthetic pathway.
  Journal
J Struct Biol 192:349-357 (2015)
DOI:10.1016/j.jsb.2015.09.013
  Sequence
[pfl:PFL_2787]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.56
IUBMB Enzyme Nomenclature: 1.14.19.56
ExPASy - ENZYME nomenclature database: 1.14.19.56
BRENDA, the Enzyme Database: 1.14.19.56

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