Entry
Name
acyl-CoA (9+3)-desaturase;
oleoyl-CoA 12-desaturase;
Delta12 fatty acid desaturase;
Delta12(omega6)-desaturase;
oleoyl-CoA Delta12 desaturase;
Delta12 desaturase;
Delta12-desaturase;
Delta12-fatty-acid desaturase;
acyl-CoA,hydrogen donor:oxygen Delta12-oxidoreductase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
BRITE hierarchy
Sysname
acyl-CoA,ferrocytochrome b5:oxygen oxidoreductase (12,13 cis-dehydrogenating)
Reaction(IUBMB)
(1) oleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = linoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O [RN:
R11043 ];
(2) palmitoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = (9Z,12Z)-hexadeca-9,12-dienoyl-CoA + 2 ferricytochrome b5 + 2 H2O [RN:
R11044 ]
Reaction(KEGG)
Substrate
Product
linoleoyl-CoA [CPD:
C02050 ];
ferricytochrome b5 [CPD:
C00996 ];
H2O [CPD:
C00001 ];
(9Z,12Z)-hexadeca-9,12-dienoyl-CoA [CPD:
C21000 ]
Comment
This microsomal enzyme introduces a cis double bond at position 12 of fatty-acyl-CoAs that contain a cis double bond at position 9. When acting on 19:1Delta10 fatty acyl-CoA the enzyme from the pathogenic protozoan Trypanosoma brucei introduces the new double bond at position 13, indicating that the new double bond is introduced three carbons from the existing cis double bond, towards the methyl-end of the fatty acid. Requires cytochrome b5 as the electron donor [4].
History
EC 1.14.19.6 created 2008, modified 2015
Pathway
ec01040 Biosynthesis of unsaturated fatty acids
Orthology
K10256 omega-6 fatty acid desaturase / acyl-lipid omega-6 desaturase (Delta-12 desaturase)
K22993 bifunctional Delta-12/omega-3 fatty acid desaturase
Genes
BCOO : 119067137 119073867 119077976 119084236 119085821
BTAB : 109031516 109034286 109037327 109038609 109038610 109038611 109043360 109043386 109043387 109043419 109043487 109043488 109043505
AMIL : 114948134 114973367
PDAM : 113667909 113682750
SPIS : 111329485 111342409
CSAT : 104745425 104764975 104776214
BNA : 106417584 106452409 106452523
RSZ : 108862913 130497099 130504985
CIC : CICLE_v10011949mg CICLE_v10015614mg
MINC : 123198158 123214921 123225237 123228010
GRA : 105761671 105783314 105785452 105785453
GHI : 107901973 107921152 107921339 107924178 107934594 107941903 107945411 121212649
GAB : 108450424 108473778 108480547 108482302
HSYR : 120153918 120158105 120183510 120183934
DZI : 111295947 111304019 111311572
GMX : 100780912(FAD2-2D) 100805040(FAD2-2B) 100805777(FAD2-1B) 100809290(FAD2-2A) 102662570 547814(FAD2-1A) 547815(FAD2-2) 732617(FAD2-3)
GSJ : 114367074 114370405 114400352 114401640 114406779
PVU : 137814138 137827760 137830634
VRA : 106757695 106770720 106771256
VAR : 108323476 108325188 108325364
VUN : 114174864 114189943 114190784
VUM : 124820456 124827773 124827774 124830459
CCAJ : 109794836 109795239 109796052 109796649
APRC : 113861993 113872359 113873750
MTR : 11444765 25483518 25485564
TPRA : 123888747 123898279 123899802
CAM : 101489419 101507051 101513688
PSAT : 127098513 127126234
VVO : 131621445 131621556 131636358
ADU : 107464693 107467337 107492893
AIP : 107616829 107619143 107645414
AHF : 112696114 112710390 112712140 112755017 112776164 112779709
LANG : 109328907 109331342 109345961 109346314 109347587
PCIN : 129301231 129303850 129319588
QSA : O6P43_004880 O6P43_005841 O6P43_008751 O6P43_009477
PMUM : 103339420 103341921
PAVI : 110763023 110770740
MDM : 103427045 103443127 103454260
MSYL : 126592667 126599950 126616101
PXB : 103958977 103963937 103964864
MNT : 21386779 21391283 21401389
CSAV : 115701196 115718446 115719000 115719251 115719329
CMAX : 111470178 111481665 111484610 111490747
CMOS : 111441899 111448352 111448353 111459302
CPEP : 111777569 111777619 111786454 111786608 111789928 111795654 111800320
HBR : 110635031 110638358 110650724
MESC : 110621646 110621802 110622513
POP : 18094295 7455763 7479530 7483979
PEU : 105111221 105131774 105134978 105134979 105134982
PALZ : 118043755 118049781 118049805 118049806 118049817 118052395
PNZ : 133677327 133677328 133682788 133688779 133692412
JRE : 109001694 109011954 109021930
CILL : 122276083 122278215 122292068
CAVE : 132164512 132164706 132164733 132191453
TWL : 119995107 119996264 119998754 120017269
SLY : 101248657 101250481 101255474 101259966 109119181 112941338 544107(cevi19)
SPEN : 107003514 107005223 107005224 107005731 107005782 107005832 107006602 107015240 107017128
SOT : 102579196 102579518 102579844 102580407 102581806 102584924 102585247 102593159 102596973 102597622 102597941 102598049 102598267 102598608 102600240
SSTN : 125856711 125858454 125862308 125862309 125866588 125866589 125866590 125866591 125866592 125866605 125872824
SDUL : 129870260 129871090 129871655 129887782 129888911 129891120 129891129 129891137 129894236 129902073 129902272 129902336 129902412 129902486 129902550
CANN : 107843168 107849596 107849612 107851251 107851421 107851434 107851473 107851685 107851744 107873633 124894986
LBB : 132602713 132602715 132602716 132602717 132603721 132603722 132603733 132603735 132626002 132635573
NTA : 107773163 107797104 107814090 107823061(FAD2) 107829678 107831844
NSY : 104226786 104230317 104246180
NTO : 104089719 104090741 104098987 104106672 104121261
NAU : 109215055 109215819 109216417 109216418 109218761 109235687
INI : 109150720 109152414 109182042
ITR : 116007185 116007582 116007943 116024476 116029392
SIND : 105159686 105163532(FAD2)
OEU : 111365935 111371390 111390764 111393798 111399693
SSPL : 121747569 121751845 121794328 121798703
SHIS : 125188161 125205310
APAN : 127258679 127258874 127263751
HAN : 110865031 110865032 110865236 110865239 110865277 110865281 110865284 110865285 110867681 110868684 110879372 110894444 110894736 110897314 110897316 110897318 110897319 110897568 110904312 110938218 110938220 110938221 110939886 110939887 110939961 110939971
ECAD : 122578445 122578764 122579373 122582919 122590523 122591112 122591269 122591356 122591604 122606452 122607353 122608062 122608674 122611246
LSV : 111879673 111882294 111884484 111885018 111889077 111889078 111889080 111889206 111890254 111896570 111896575 111896578 111896579 111896580 111896581 111896582 111897874 111899826 111900952 111900953 111904205 111908893 111909558 111915490 111918622
CCAV : 112505457 112505482 112505726 112506618 112506654 112506812 112506837 112506966 112506967 112506968 112512117 112513526 112521107 112521212 112521380 112521518 112521640 112521907 112521908 112521909 112521958 112525343 112526112 112527377 112527536 112527987 112528359 112528364
DCR : 108193493 108194523 108197230 108197256 108197419 108198633 108198634 108198635 108198637 108198638 108199375 108202677 108204656 108204690 108207522 108210933 108211018 108212237 108217089 108217440 108218481 108218482 108218742 108218743 108219326 108220169 108220600 108220695 108221065 108221821 108221989 108222165 108222186 108223766 108223875 108224006 108225347 108227781
CSIN : 114267865 114282301
RVL : 131315019 131315020 131324332 131324333
CQI : 110689244 110695943 110734516 110736134
ATRI : 130796765 130820812 130825871
MOF : 131148005 131165865 131167705 131167706 131167708
NNU : 104597556 104603541 104611778
MING : 122061917 122087967
PSOM : 113311026 113322906
OSA : 4330523 4343026 4343027
OGL : 127762104 127780007 127780184
ATS : 109756354 109768827 109774922 109774938 109781985
TDC : 119317653 119317654 119321176 119322537 119326524
TAES : 123128186 123130860 123130862 123134421 123134423 123138008 123145288 123145289 123145293 123145295
TUA : 125512392 125512434 125514359 125514360
LPER : 127306411 127311774 127311775
SBI : 110435045 8060698 8066368
ZMA : 100191328 100273207 100282744 100381639 103631986 103640919 118475586
MFLO : 136467571 136467616 136474671 136477988 136542987
SITA : 101768137 101783555
PVIR : 120650240 120657027 120677804 120701919
PHAI : 112886426 112890525 112896900
MUS : 103976995 103980416 135583191 135625871
ZOF : 121993200 121997168 122020013 122023662 122032659 122035107
AOF : 109833613 109843629 109843630
ACAK : 137945507 137949569
TDL : TDEL_0E01510(TDEL0E01510)
LBG : 92209539(LODBEIA_P43430)
CAL : CAALFM_C601110WA(FAD2)
CTEN : 18247366(PSN45_004733)
PKZ : C5L36_0C09550 C5L36_0C09560
YLI : 2906773(YALI2_C00885g)
NCR : NCU02209(ods) NCU09497
NTE : NEUTE1DRAFT116751(NEUTE1DRAFT_116751) NEUTE1DRAFT86398(NEUTE1DRAFT_86398)
SMP : 10802522(SMAC4_07882) 10808924(SMAC4_03650)
PBEL : QC761_504330(FAH12) QC761_701450
PPSD : QC762_504330(FAH12) QC762_701450
PPSP : QC763_504330(FAH12) QC763_701450
PPSA : QC764_504330(FAH12) QC764_701450
MTM : MYCTH_50837 MYCTH_88639
CTHR : CTHT_0000240 CTHT_0071750
PPEI : PpBr36_10589 PpBr36_10691
PGRI : PgNI_00111 PgNI_05225
TMN : UCRPA7_3220 UCRPA7_5047
SSCK : SPSK_00128 SPSK_07620
FGR : FGSG_05784 FGSG_07890
FPU : FPSE_04515 FPSE_11043
FPOA : FPOAC1_007204 FPOAC1_007328 FPOAC1_008748(FAH12) FPOAC1_011680 FPOAC1_013164 FPOAC1_013185 FPOAC1_013410 FPOAC1_013533 FPOAC1_013653 FPOAC1_013914 FPOAC1_013916
FVN : FVRRES_09344 FVRRES_12734
FVR : FVEG_05830 FVEG_12065
FOX : FOXG_03793 FOXG_08835
NHE : NECHADRAFT_102202 NECHADRAFT_66003
FFC : NCS54_00197700 NCS54_00495900
FKR : NCS57_00193500 NCS57_00533700
FMU : J7337_004932(FAH12) J7337_007239
TRE : TRIREDRAFT_45250 TRIREDRAFT_74228
TRR : M419DRAFT_108308 M419DRAFT_26391
TATV : 25780586(TrAtP1_009991) 25780669(TrAtP1_007277)
TASP : 36608895(FAH12) 36611189(TrAFT101_011208)
MBRN : 26245143(FAH12_0) 26246728(FAH12_1)
AMUS : LMH87_000016 LMH87_010184
PLJ : 28892358(PLICBS_000183) 28892565(PLICBS_006409)
PTKZ : JDV02_003432(FAH12) JDV02_008161
VDA : VDAG_02971 VDAG_10469
CFJ : CFIO01_04749 CFIO01_09629
CLUP : CLUP02_11556 CLUP02_16261 CLUP02_17062
CHIG : CH63R_11053 CH63R_12552
CDET : 87942720(CDEST_06217) 87945815(CDEST_09312)
SAPO : SAPIO_CDS0643 SAPIO_CDS7954
ELA : UCREL1_10483 UCREL1_10881 UCREL1_5610
PFY : PFICI_02105 PFICI_05540 PFICI_11937
SSL : SS1G_03438 SS1G_13683
BFU : BCIN_01g05120 BCIN_01g10750 BCIN_02g06310 BCIN_13g00280
PSCO : LY89DRAFT_613354 LY89DRAFT_693845 LY89DRAFT_706947
GLZ : GLAREA_04493 GLAREA_09934
ANI : ANIA_01037 ANIA_07204
AFM : AFUA_1G12530 AFUA_6G03050
ACT : ACLA_022950 ACLA_097340
NFI : NFIA_013020 NFIA_049390
AOR : AO090001000224 AO090010000714
ANG : An01g14970 An08g05160 An14g06980
AFV : AFLA_008570 AFLA_014214
ALUC : AKAW2_20019A AKAW2_30542S(FAH12) AKAW2_41307S
APUU : APUU_20896A APUU_50747S(FAH12)
PCS : N7525_002119 N7525_002710 N7525_003929
PDP : PDIP_27200 PDIP_61260
POU : POX_c04082 POX_d06013
TRG : TRUGW13939_09539 TRUGW13939_10884
URE : UREG_02885 UREG_03614
PTRR : 6341855(PtrM4_096950)
BZE : COCCADRAFT_35692 COCCADRAFT_95357
BSC : COCSADRAFT_255949 COCSADRAFT_263376
BOR : COCMIDRAFT_80232 COCMIDRAFT_99684
AALT : CC77DRAFT_261211 CC77DRAFT_674296 CC77DRAFT_946470
ARAB : EKO05_0004585 EKO05_0008621
PFJ : MYCFIDRAFT_72590 MYCFIDRAFT_86366
FFU : CLAFUR5_03699 CLAFUR5_05071
CBET : CB0940_02620 CB0940_11393
NPA : UCRNP2_9247 UCRNP2_969
CDEP : 91084370(L203_100154)
KMG : 30163164(I203_102154)
KNE : 92180031(IAR55_002773)
TASA : A1Q1_01239 A1Q1_06326
CCAC : CcaHIS019_0112250(CcaverHIS019_0112250) CcaHIS019_0508550(CcaverHIS019_0508550)
TVS : TRAVEDRAFT_147014 TRAVEDRAFT_28463 TRAVEDRAFT_38834
RSX : RhiXN_08914 RhiXN_08917
LBC : LACBIDRAFT_292603(FAD12-5)
ABP : AGABI1DRAFT113988(AGABI1DRAFT_113988)
ABV : AGABI2DRAFT193825(AGABI2DRAFT_193825)
PCUB : JR316_0007061 JR316_0012985
MORE : E1B28_012456 E1B28_013509
SCM : SCHCO_02615271(SCHCODRAFT_02615271)
PGR : PGTG_00088 PGTG_00121
PTRC : PtA15_1A110 PtA15_1A111 PtA15_1A125
PSTR : Pst134EA_001028 Pst134EA_001035 Pst134EA_001054 Pst134EA_004855 Pst134EA_004856
MLR : MELLADRAFT_70376 MELLADRAFT_75337
LMAT : 92512922(LSCM1_02844)
LOI : 92358618(LSCM4_02658)
LEIS : 94178061(GH5_02552)
LENR : 94169385(CUR178_02112)
LEIN : 94185864(JIQ42_02137)
» show all
Taxonomy
Reference
Authors
Borgeson CE, de Renobales M, Blomquist GJ.
Title
Characterization of the delta 12 desaturase in the American cockroach, Periplaneta americana: the nature of the substrate.
Journal
Reference
Authors
Lomascolo A, Dubreucq E, Galzy P.
Title
Study of the delta 12-desaturase system of Lipomyces starkeyi.
Journal
Reference
Authors
Tocher DR, Leaver MJ, Hodgson PA.
Title
Recent advances in the biochemistry and molecular biology of fatty acyl desaturases.
Journal
Reference
Authors
Petrini GA, Altabe SG, Uttaro AD
Title
Trypanosoma brucei oleate desaturase may use a cytochrome b5-like domain in another desaturase as an electron donor.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 1.14.19.6