KEGG   ENZYME: 1.14.19.61
Entry
EC 1.14.19.61               Enzyme                                 
Name
dihydrorhizobitoxine desaturase;
rtxC (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
dihydrorhizobitoxine,ferredoxin:oxygen oxidoreductase (3,4 trans-dehydrogenating)
Reaction(IUBMB)
dihydrorhizobitoxine + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = rhizobitoxine + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O [RN:R12074]
Reaction(KEGG)
R12074
Substrate
dihydrorhizobitoxine [CPD:C21888];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
O2 [CPD:C00007];
H+ [CPD:C00080]
Product
rhizobitoxine [CPD:C21887];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Bradyrhizobium elkanii, catalyses the final step in the biosynthesis of the nodulation enhancer compound rhizobitoxine.
History
EC 1.14.19.61 created 2018
Orthology
K22875  dihydrorhizobitoxine desaturase
Genes
RBQJ2J99_29540
RBANJ2J98_27365
RRGJ3P73_34775
BJAblr2080(rtxC)
BJUBJ6T_77760
BJPRN69_37750
BRCBCCGELA001_28815
BOTCIT37_02595
BARHWN72_10955
BVZBRAD3257_7047(rtxC)
BELBE61_76710(rtxC)
BDGLPJ38_35695
 » show all
Reference
1  [PMID:11679318]
  Authors
Yasuta T, Okazaki S, Mitsui H, Yuhashi K, Ezura H, Minamisawa K
  Title
DNA sequence and mutational analysis of rhizobitoxine biosynthesis genes in Bradyrhizobium elkanii.
  Journal
Appl Environ Microbiol 67:4999-5009 (2001)
DOI:10.1128/AEM.67.11.4999-5009.2001
  Sequence
Reference
2  [PMID:14711685]
  Authors
Okazaki S, Sugawara M, Minamisawa K
  Title
Bradyrhizobium elkanii rtxC gene is required for expression of symbiotic phenotypes in the final step of rhizobitoxine biosynthesis.
  Journal
Appl Environ Microbiol 70:535-41 (2004)
DOI:10.1128/AEM.70.1.535-541.2004
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.61
IUBMB Enzyme Nomenclature: 1.14.19.61
ExPASy - ENZYME nomenclature database: 1.14.19.61
BRENDA, the Enzyme Database: 1.14.19.61

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