| Entry |
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| Name |
berbamunine synthase;
(S)-N-methylcoclaurine oxidase (C-O phenol-coupling)
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| Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
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| Sysname |
(S)-N-methylcoclaurine,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (C-O phenol-coupling)
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| Reaction(IUBMB) |
(S)-N-methylcoclaurine + (R)-N-methylcoclaurine + [reduced NADPH---hemoprotein reductase] + O2 = berbamunine + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN: R04694]
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| Reaction(KEGG) |
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| Substrate |
(S)-N-methylcoclaurine [CPD: C05176];
(R)-N-methylcoclaurine [CPD: C05243];
[reduced NADPH---hemoprotein reductase] [CPD: C03024];
O2 [CPD: C00007]
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| Product |
berbamunine [CPD: C05177];
[oxidized NADPH---hemoprotein reductase] [CPD: C03161];
H2O [CPD: C00001]
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| Comment |
A cytochrome P-450 (heme-thiolate) protein found in plants. Forms the bisbenzylisoquinoline alkaloid berbamunine by phenol oxidation of N-methylcoclaurine without the incorporation of oxygen into the product. Reaction of two molecules of (R)-N-methylcoclaurine gives the dimer guattagaumerine.
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| History |
EC 1.14.19.66 created 1999 as EC 1.1.3.34, transferred 2002 to EC 1.14.21.3, transferred 2018 to EC 1.14.19.66
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| Pathway |
| ec00950 | Isoquinoline alkaloid biosynthesis |
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| Orthology |
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| Reference |
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| Authors |
Stadler R, Zenk MH. |
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| Title |
The purification and characterization of a unique cytochrome P-450 enzyme from Berberis stolonifera plant cell cultures. |
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| Journal |
J Biol Chem 268:823-31 (1993) |
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| Other DBs |
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