KEGG ENZYME: 1.14.19.8

ENZYME: 1.14.19.8

Entry

EC 1.14.19.8                Enzyme

Name

pentalenolactone synthase;
penM (gene name);
pntM (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water

Sysname

pentalenolactone-reduced-ferredoxin:oxygen oxidoreductase (pentalenolactone-forming)

Reaction(IUBMB)

pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+ = pentalenolactone + 2 oxidized ferredoxin + 2 H2O [RN:R10162]

Reaction(KEGG)

R10162

Substrate

pentalenolactone F [CPD:C20397];
O2 [CPD:C00007];
reduced ferredoxin [CPD:C00138];
H+ [CPD:C00080]

Product

pentalenolactone [CPD:C20407];
oxidized ferredoxin [CPD:C00139];
H2O [CPD:C00001]

Comment

A heme-thiolate protein (P-450). Isolated from the bacteria Streptomyces exfoliatus and Streptomyces arenae.

History

EC 1.14.19.8 created 2012 as EC 1.3.7.10, transferred 2013 to EC 1.14.19.8

Pathway

ec00998

Biosynthesis of various antibiotics

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K17476

pentalenolactone synthase

Genes

SBH:	SBI_09672(cyp47)

SCW:	TU94_27370

SLE:	sle_13230(sle_13230)

SSIA:

A7J05_09275

SGJ:	IAG43_27120

SHK:	J2N69_35840

Reference

1 [PMID:21284395]

Authors

Zhu D, Seo MJ, Ikeda H, Cane DE

Title

Genome mining in streptomyces. Discovery of an unprecedented P450-catalyzed oxidative rearrangement that is the final step in the biosynthesis of pentalenolactone.

Journal

J Am Chem Soc 133:2128-31 (2011)
DOI:10.1021/ja111279h

Sequence

[ag:ADO85587 ADO85571]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.19.8

IUBMB Enzyme Nomenclature:

1.14.19.8

ExPASy - ENZYME nomenclature database:

1.14.19.8

BRENDA, the Enzyme Database:

1.14.19.8

DBGET integrated database retrieval system