KEGG ENZYME: 1.14.20.6

ENZYME: 1.14.20.6

Entry

EC 1.14.20.6                Enzyme

Name

flavonol synthase;
FLS (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and the other dehydrogenated

Sysname

dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase

Reaction(IUBMB)

a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]

Reaction(KEGG)

R07368 > R02160 R03126 R06539 R08082

Substrate

dihydroflavonol [CPD:C15570];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]

Product

flavonol [CPD:C01495];
succinate [CPD:C00042];
CO2 [CPD:C00011];
H2O [CPD:C00001]

Comment

In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.

History

EC 1.14.20.6 created 2004 as EC 1.14.11.23, transferred 2018 to EC 1.14.20.6

Pathway

ec00941

Flavonoid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K05278

flavonol synthase

Genes

ATH:	AT5G08640(FLS1) AT5G43935(FLS6) AT5G63580(FLS2) AT5G63590(FLS3) AT5G63595(FLS4) AT5G63600(FLS5)

ALY:	9300932 9300933 9300934 9301468 9302635 9309443 9316690 9317565

CRB:	17874951 17874978 17875062 17875365 17875649 17876064 17876240 17876394 17876899 17884061 17887601

CSAT:

104708215 104725029 104726949 104726979 104726980 104726983 104726984 104726985 104726986 104727463 104729053 104734897 104741256 104741513 104741522 104741531 104741542 104741553 104741561 104741572 104741592 104741603 104762497 104762498 104762499 104762501 104762503 104769085 104778112 104784343 109124872

EUS:	EUTSA_v10004662mg EUTSA_v10004668mg EUTSA_v10005710mg EUTSA_v10014054mg EUTSA_v10028827mg EUTSA_v10029534mg

BRP:	103837496 103847177 103855056 103855057 103873165 103873769 103873770

BNA:	106351880 106351881 106382906 106409586 106419433 111201386 125587543 125587544 125592317 125598042 125605797 125610301

BOE:	106313418 106316306 106320382 106328324 106332816 106341567

RSZ:	108806153 108812047 108814290 108839020 130507647

THJ:

104819356

CPAP:

110816514

CIT:	102618406 102626432 102629970

CIC:	CICLE_v10003741mg CICLE_v10015856mg CICLE_v10026028mg

PVY:	116110546 116110548 116110558 116133801 116145683

MINC:

123199612 123199749 123199750 123202765 123202986 123206089 123206097 123218347

TCC:	18592290 18592292 18599277

GRA:	105770783 105780413 105780414

GHI:	107904987 107923058 107940452 107940456 107959505 107959507

GAB:	108452122 108457975 108459765

DZI:	111275455 111314969

EGR:	104428803 104444489 104450481 104450482

GMX:	100810937 100816829 778157(FLS1)

GSJ:	114380973 114412340 114414096

PVU:	PHAVU_004G052400g PHAVU_008G1660000

VRA:	106763888 106769199

VAR:	108337532 108345477

VUN:	114180972 114193841

CCAJ:

109804443 109808020 109816831

APRC:

113848076 113848139 113853130

MTR:	11415879 11415909 11435413 11435414

TPRA:

123896878 123907678

CAM:	101504604 101509377

PSAT:

127088203 127118888 127118889

LJA:	Lj1g3v0705350.1(Lj1g3v0705350.1) Lj1g3v0775780.1(Lj1g3v0775780.1) Lj1g3v1380920.1(Lj1g3v1380920.1) Lj5g3v1865700.1(Lj5g3v1865700.1)

ADU:	107458261 107465302

AIP:	107609791 107617070

AHF:	112702733 112712950 112766372 112777054

LANG:

109326685 109356468 109357523 109360027

PCIN:

129294605 129296392 129298443 129298446 129299102 129319819

FVE:	101295969 101296827

RCN:	112170431 112173037

PPER:

18788166 18788410 18789071 18792331

PMUM:

103326444 103332906 103332909 103332910

PAVI:

110753589 110753698 110754141

PDUL:

117613713 117613942 117628886 117628896

MDM:	103413102(FLS) 103441195 114821553 114821784

MSYL:

126601692 126604485 126632407

PXB:	103933697 103956721

ZJU:	107415994 107421006

MNT:	21396296 21403067 21406496 21406497 21406498

CSAV:

115708857 115717395

CSV:

101219245

CMO:

103488630

BHJ:

120075949

MCHA:

111011799

CMAX:

111486888 111489355

CMOS:

111444648 111464499

CPEP:

111780272 111809053

RCU:	8274085 8274087 8275129

JCU:

105639310

HBR:	110654972 110655043 110665573

MESC:

110606120 110607889 110609989

POP:	18097776 18109811 7458869 7495105

PEU:	105108614 105114941 105116982 105127840

PALZ:

118041763 118050782 118050795 118051939 118058366

JRE:	108981974 108982002 108988364 108991214 109012769 118349241

CILL:

122305768 122306677 122309083 122309313 122309415

CAVE:

132166160 132166161 132166886 132167416 132167442

QSU:	112003934 112004171 112033039

QLO:	115959418 115963835 115973387 115993005

TWL:	120003005 120010136

VVI:	100232938(FLS1) 100232939 100243852 100249002 100267819

VRI:	117905961 117906026 117906419 117907623

SLY:

101249699

SPEN:

107004995

SOT:	102577717(FLS)

SSTN:

125844784

CANN:

107843086 107869735

LBB:

132627034

NTA:	107794305(FLS1) 107814657(NtFLS)

NSY:

104236078

NTO:

104101386

NAU:

109237082

INI:

109175998

ITR:

116002506

SIND:

105155397 105176681

OEU:	111397470 111409089

EGT:

105974437

SSPL:

121795166

SMIL:

131011922 131011924 131011976 131011977

APAN:

127257191

HAN:	110878613 110878615

ECAD:

122587212

LSV:

111900153

CCAV:

112515207 112515212

DCR:	108203535 108221899

CSIN:

114257498 114257531 114283320 114314253

RVL:	131303780 131307531 131329983 131329984

AEW:	130758149 130776385 130786229

BVG:	104883093 104901038

SOE:	110778214 110795044

CQI:	110693494 110714529 110725334

MOF:	131161887 131162287

NNU:	104596714 104608517 104609672 104609673

MING:

122068147 122072145 122075321 122076036 122076177

TSS:	122662359 122663411

PSOM:

113346311 113346474 113346476 113356202 113356203 113361278

OSA:

4330843

DOSA:

Os02t0767300-01(Os02g0767300)

OBR:

102712047

OGL:	127755732 127764721

BDI:

100831956

ATS:	109764595 109768154 109784747

TDC:	119294168 119304118 119318079 119321892

TAES:

123089513 123098991 123108371 123131062 123134660 123141888

TUA:	125511422 125512561 125512649

LPER:

127305944 127319118 127336784

LRD:	124670551 124674562 124677808 124688482

SBI:

8078926

ZMA:	100272992 103627785

SITA:

101780580

SVS:

117842212

PVIR:

120642963 120653872

PHAI:

112882371

PDA:

103712517

EGU:

105058232

MUS:	103969181 103969182 103977541 103995223

ZOF:	121971547 121976111 121990229 121992545 121996484 121996485

PEQ:

110019794

AOF:

109820207

NCOL:

116259344 116261229

ATR:	18425268 18425281 18426066

» show all

Reference

1 [PMID:12180990]

Authors

Wellmann F, Lukacin R, Moriguchi T, Britsch L, Schiltz E, Matern U

Title

Functional expression and mutational analysis of flavonol synthase from Citrus unshiu.

Journal

Eur J Biochem 269:4134-42 (2002)
DOI:10.1046/j.1432-1033.2002.03108.x

Sequence

[ag:BAA36554]

Reference

2 [PMID:12620339]

Authors

Lukacin R, Wellmann F, Britsch L, Martens S, Matern U.

Title

Flavonol synthase from Citrus unshiu is a bifunctional dioxygenase.

Journal

Phytochemistry 62:287-92 (2003)
DOI:10.1016/S0031-9422(02)00567-8

Reference

3 [PMID:12782296]

Authors

Martens S, Forkmann G, Britsch L, Wellmann F, Matern U, Lukacin R

Title

Divergent evolution of flavonoid 2-oxoglutarate-dependent dioxygenases in parsley.

Journal

FEBS Lett 544:93-8 (2003)
DOI:10.1016/S0014-5793(03)00479-4

Sequence

[ag:AAP57395]

Reference

4 [PMID:14570878]

Authors

Turnbull JJ, Nakajima J, Welford RW, Yamazaki M, Saito K, Schofield CJ.

Title

Mechanistic studies on three 2-oxoglutarate-dependent oxygenases of flavonoid biosynthesis: anthocyanidin synthase, flavonol synthase, and flavanone 3beta-hydroxylase.

Journal

J Biol Chem 279:1206-16 (2004)
DOI:10.1074/jbc.M309228200

Other DBs

ExplorEnz - The Enzyme Database:

1.14.20.6

IUBMB Enzyme Nomenclature:

1.14.20.6

ExPASy - ENZYME nomenclature database:

1.14.20.6

BRENDA, the Enzyme Database:

1.14.20.6

CAS:	146359-76-4

DBGET integrated database retrieval system