KEGG   ENZYME: 1.14.20.9
Entry
EC 1.14.20.9                Enzyme                                 
Name
L-tyrosine isonitrile desaturase;
pvcB (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and the other dehydrogenated
Sysname
(2S)-3-(4-hydroxyphenyl)-2-isocyanopropanoate,2-oxoglutarate:oxygen oxidoreductase
Reaction(IUBMB)
(2S)-3-(4-hydroxyphenyl)-2-isocyanopropanoate + 2-oxoglutarate + O2 = (2E)-3-(4-hydroxyphenyl)-2-isocyanoprop-2-enoate + succinate + CO2 + H2O [RN:R12081]
Reaction(KEGG)
R12081
Substrate
(2S)-3-(4-hydroxyphenyl)-2-isocyanopropanoate [CPD:C21889];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
(2E)-3-(4-hydroxyphenyl)-2-isocyanoprop-2-enoate [CPD:C21891];
succinate [CPD:C00042];
CO2 [CPD:C00011];
H2O [CPD:C00001]
Comment
The enzyme is a member of the Fe2+, 2-oxoglutarate-dependent oxygenases and requires Fe2+. It has been characterized from bacteria that form the isonitrile-functionalized compound paerucumarin. cf. EC 1.14.20.10, L-tyrosine isonitrile desaturase/decarboxylase.
History
EC 1.14.20.9 created 2018
Pathway
ec00997  Biosynthesis of various other secondary metabolites
Orthology
K22874  L-tyrosine isonitrile desaturase/decarboxylase
Genes
SMARSM39_0024(pvcB)
SMACSMDB11_0022(pvcB)
SMWSMWW4_v1c06960
SPESpro_0704
SRRSerAS9_0644
SRLSOD_c05760
SRYM621_03040
SPLYQ5A_003040
SRSSerAS12_0644
SRASerAS13_0644
SLQM495_02880
SERFL085_00780
SQUE4343_13095
SFJSAMEA4384070_0732
SSURATE40_000110
SURIJ0X03_20645
SENPKHA73_03085
FSMCCS41_08785
ECAECA3380(pvcB)
PATREV46_16840
PATOGZ59_34170(pvcB)
PCTPC1_2908 PC1_3224
PCCPCC21_029460
PCVBCS7_14800 BCS7_16165
PWAPecwa_1104
PPARA8F97_13425
PECW5S_0996
PWSA7983_12895
PPOABJK05_09650
PBRAB5S52_07100
PPUJE2566_16435
PARII2D83_05690 I2D83_07055
PAQUDMB82_0006950
PVZOA04_31220
PPAVLOZ86_14585 LOZ86_16795
PQUIG609_006085
EAMEAMY_1787(pvcB)
EAYEAM_1750(pvcB)
ERJEJP617_29220(pvcB)
PLUplu2817
PLUMA4R40_14045
PAYPAU_01720(pvcB)
PTTVY86_17220
PAKHB0X70_14360
PLUICE143_14355
XBOXBJ1_1354
XBVXBW1_1622
XNEXNC1_1222
XNMXNC2_1198(isnB)
XDOXDD1_2892
XPOXPG1_0844
XHOA9255_00175
XBUHGO23_14995
LEZGLE_4812
VCHVC_1944
VCFIR04_14875
VCSMS6_1705
VCEVch1786_I1437(pvcB)
VCQEN18_12835
VCJVCD_002418
VCIO3Y_09405
VCOVC0395_A1534(pvcB)
VCRVC395_2059(pvcB)
VCMVCM66_1868(pvcB)
VCLVCLMA_A1689
VCXVAA049_932
VCZVAB027_2586
VNIVIBNI_A3570(pvcB)
VANVAA_02643
LAGN175_02885
VAUVANGNB10_cI2373c(pvcB)
VQICCZ37_11640
PAEPA2255(pvcB)
PAEVN297_2328(pvcB)
PAEIN296_2328(pvcB)
PAUPA14_35420(pvcB)
PAPPSPA7_2986(pvcB)
PAGPLES_30491(pvcB)
PAFPAM18_2785(pvcB)
PNCNCGM2_3265(pvcB)
PAEBNCGM1900_4275(pvcB)
PDKPADK2_14000
PSGG655_13765
PRPM062_11795
PAEPPA1S_14345
PAERPA1R_gp0056
PAEMU769_13930
PAELT223_15575
PAESSCV20265_3071
PAEUBN889_02461(pvcB)
PAEGAI22_19510
PAECM802_2325(pvcB)
PAEOM801_2327(pvcB)
PLQAA042_05065
PTRTHU722_0010625
ACDAOLE_01055
SPSHFM037_15215
PPISB1L02_15130
PSENPNC201_02370(carC)
MICZGL2_40870
ALGAQULUS_02970(ptlD)
ASIPAQUSIP_24820(sdpA)
LPNlpg0175
LPHLPV_0258(pvcB)
LPOLPO_0206(pvcB)
LPULPE509_03055
LPMLP6_0179(pvcB)
LPFlpl0237
LPPlpp0237
LPCLPC_0257(pvcB)
LPAlpa_00342
LPElp12_0177
LLOLLO_2213
LSHCAB17_16390
LLG44548918_00375
LIBE4T55_11460
LCJNCTC11976_00381
LSSNCTC12082_02497
THIPN838_14005
TOLTOL_3183
TORR615_14965
AHAAHA_3281
AHYAHML_17545
AHDAI20_02950
AHRV428_18200
AHPV429_18235
AHJV469_05060
AHHRY45_16925
AHIVU14_05265
AAJBOQ57_16800
ASAASA_1025(pvcB)
AEOO23A_p0208
ADHCK627_09305
BMABMAA1923(pvcB)
BMVBMASAVP1_0938(pvcB)
BMLBMA10229_1223(pvcB)
BMNBMA10247_A2200(pvcB)
BMALDM55_3864(pvcB)
BMAEDM78_4783(pvcB)
BMAQDM76_4561(pvcB)
BMAIDM57_06430
BMAFDM51_3611
BMAZBM44_3823(pvcB)
BMABBM45_4600
BPSBPSS0161
BPMBURPS1710b_A1680(pvcB)
BPLBURPS1106A_A0229(pvcB)
BPDBURPS668_A0319(pvcB)
BPSEBDL_6052(pvcB)
BPSMBBQ_6053(pvcB)
BPSUBBN_3541(pvcB)
BPSDBBX_5222(pvcB)
BPZBP1026B_II0181
BPQBPC006_II0218
BPKBBK_5353(pvcB)
BPSHDR55_5201(pvcB)
BPSABBU_3411(pvcB)
BPSOX996_4969(pvcB)
BUTX994_4518
BTEBTH_II0230(pvcB)
BTQBTQ_3525(pvcB)
BTJBTJ_4562(pvcB)
BTZBTL_5349(pvcB)
BTVBTHA_4852(pvcB)
BTHEBTN_5020(pvcB)
BTHMBTRA_5288(pvcB)
BTHADR62_5150
BTHLBG87_5355
BOKDM82_3790(pvcB)
BOCBG90_4253
BUUWS70_27860
BSAVWS86_29765
BUBBW23_5843
BSTGWT74_28465
SECHB18_23350
BBABd1455(pvcB)
BBATBdt_1441(pvcB)
BBACEP01_03535
BDQCIK05_01010
MMAUNCTC10168_00532
NBRO3I_007380
NIEKV110_36470
NGPLTT66_00580
SALSSLNWT_6359
STREGZL_02118
SRNA4G23_02273(tfdA)
SCADDN051_33295
SNRSNOUR_09500
SRKFGW37_29470
SBYH7H31_08600 H7H31_12645
SRIMCP984_10750
SBATG4Z16_03990
SFBCP974_12515
FRAFrancci3_2755
AABA4R43_21560
KALKALB_6298
UAMUABAM_02082
HSWHsw_PA0222
FSDLXD69_13600
 » show all
Reference
1  [PMID:18689486]
  Authors
Clarke-Pearson MF, Brady SF
  Title
Paerucumarin, a new metabolite produced by the pvc gene cluster from Pseudomonas  aeruginosa.
  Journal
J Bacteriol 190:6927-30 (2008)
DOI:10.1128/JB.00801-08
Reference
2  [PMID:18824174]
  Authors
Drake EJ, Gulick AM
  Title
Three-dimensional structures of Pseudomonas aeruginosa PvcA and PvcB, two proteins involved in the synthesis of 2-isocyano-6,7-dihydroxycoumarin.
  Journal
J Mol Biol 384:193-205 (2008)
DOI:10.1016/j.jmb.2008.09.027
  Sequence
[pae:PA2255]
Reference
3  [PMID:25866990]
  Authors
Zhu J, Lippa GM, Gulick AM, Tipton PA
  Title
Examining Reaction Specificity in PvcB, a Source of Diversity in Isonitrile-Containing Natural Products.
  Journal
Biochemistry 54:2659-69 (2015)
DOI:10.1021/acs.biochem.5b00255
  Sequence
[pae:PA2255]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.20.9
IUBMB Enzyme Nomenclature: 1.14.20.9
ExPASy - ENZYME nomenclature database: 1.14.20.9
BRENDA, the Enzyme Database: 1.14.20.9

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