KEGG ENZYME: 1.14.99.15

ENZYME: 1.14.99.15

Entry

EC 1.14.99.15               Enzyme

Name

4-methoxybenzoate monooxygenase (O-demethylating);
4-methoxybenzoate 4-monooxygenase (O-demethylating);
4-methoxybenzoate O-demethylase;
p-anisic O-demethylase;
piperonylate-4-O-demethylase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous

Sysname

4-methoxybenzoate,hydrogen-donor:oxygen oxidoreductase (O-demethylating)

Reaction(IUBMB)

4-methoxybenzoate + reduced acceptor + O2 = 4-hydroxybenzoate + formaldehyde + acceptor + H2O [RN:R01306]

Reaction(KEGG)

R01306

Substrate

4-methoxybenzoate [CPD:C02519];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]

Product

4-hydroxybenzoate [CPD:C00156];
formaldehyde [CPD:C00067];
acceptor [CPD:C00028];
H2O [CPD:C00001]

Comment

The bacterial enzyme consists of a ferredoxin-type protein and an iron-sulfur flavoprotein (FMN). Also acts on 4-ethoxybenzoate, N-methyl-4-aminobenzoate and toluate. The fungal enzyme acts best on veratrate.

History

EC 1.14.99.15 created 1972

Pathway

ec00362

Benzoate degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K22553

4-methoxybenzoate monooxygenase (O-demethylating)

Genes

PPU:

PP_1950

PPSH:

G5J76_09125

BHZ:	ACR54_04430

PUD:	G9Q38_04420 G9Q38_04600

PIG:	EGT29_07245

HYR:	BSY239_2681

HYL:	LPB072_21880

HCZ:	G9Q37_15470

HYDA:

ABSY19_21615

XYG:	R9X41_16735

MAMO:

A6B35_30200

AVI:

Avi_7683

NGG:	RG540_PA03940

BJA:	blr1048(blr1048)

BJU:	BJ6T_10730

BJP:	RN69_05235

BRA:

BRADO6810

BBT:

BBta_0729

BRS:

S23_67580

AOL:

S58_06520

BRC:	BCCGELA001_34415

BIC:	LMTR13_36460

BRO:	BRAD285_6957

BRK:	CWS35_02050

BOT:	CIT37_09060

BRQ:	CIT40_30200

BGQ:	X265_34795 X265_38440

BGZ:	XH91_37640

BSYM:

CIT39_29310

BBET:

F8237_13145

BARH:

WN72_37625 WN72_46340

BVZ:	BRAD3257_8678

BEL:	BE61_89430 BE61_90500

BDG:	LPJ38_02285 LPJ38_14515

BSEP:

HAP48_0012045 HAP48_0019950

BQB:	J4P68_0015900 J4P68_0023470

BBAN:

J4G43_039075 J4G43_050840

BCAN:

BcanWSM471_07645 BcanWSM471_35580

BAUT:

QA635_04745 QA635_38060

BBRA:

QA636_04220 QA636_39465

BGK:	IC762_32605

BCOU:

IC761_34775

BPAH:

QA639_04165 QA639_38410

BXN:	I3J27_28330 I3J27_37730

BROS:

QUH67_33750

BRAZ:

LRP30_35015 LRP30_42290

BOY:	LQG66_26975

BRAY:

ML401_32800 ML401_39090

RPA:	TX73_009625

RPB:

RPB_3613

RPT:

Rpal_2077

RPX:	Rpdx1_3652

RBK:	E0H22_10435

VGO:	GJW-30_1_04086

TRB:	HB776_06670

TALZ:

RPMA_19445

TARD:

RSO67_00425

MTUN:

MTUNDRAET4_0240.1(cyp199a)

PHR:	C6569_17635

PSTG:

E8M01_28530

PAQT:

E8L99_19475

PABY:

Ga0080574_TMP462

SPSE:

SULPSESMR1_04506

OCD:	FHY55_02300

HML:	HmaOT1_13885

PPRU:

FDP22_01200

PPAF:

I8N54_16495

HDH:	G5B40_05810

FRB:	AB6B37_07375

NRE:	BES08_14180

SWI:

Swit_3269

SPHD:

HY78_09210

SARI:

H5J25_19695

SJP:	SJA_C2-04160

SCH:	Sphch_3292

SSY:

SLG_20150

SPHB:

EP837_03852

SFLA:

SPHFLASMR4Y_02994

SPHY:

CHN51_15135

ACOB:

P0Y56_10080

AAY:	WYH_02150(bioI)

GTI:	FXF46_11520

KOI:	LV478_11250

ACEK:

FLP30_09705

SNEA:

NBZ79_07865

MICA:

P0L94_13155

MPHU:

MPHO_51270

CMD:	B841_09435

CCOE:

CETAM_04400

RHA:	RHA1_ro02948

ROP:	ROP_26710 ROP_33150

ROA:	Pd630_LPD07185

RAV:	AAT18_16735

RRZ:	CS378_04375

RKO:	JWS14_13820 JWS14_15455

ROZ:	CBI38_28845

RPSK:

JWS13_29175 JWS13_31765

RHOU:

KZJ41_17825

RHOW:

A4U64_07990

RHOJ:

JVH1_22255 JVH1_38050

RHOS:

QWW67_06475

RHOQ:

QWW98_06480

RHOY:

QNA09_12395

RHJS:

OYT95_23515

GPO:	GPOL_c45240

GOM:	D7316_02823

GAM:	GII34_05715

GOI:	LK459_08650 LK459_20835 LK459_20945

SGRF:

SGFS_023420

SALS:

SLNWT_0027

SLE:	sle_61820(sle_61820)

SPHW:

NFX46_17420

SGE:	DWG14_00501

SGZ:	C0216_11925

SRK:	FGW37_30985

SPHV:

F9278_42100

SBAT:

G4Z16_06250

SXN:	IAG42_02270

SDUR:

M4V62_39870

KBU:	Q4V64_03985

SMIB:

SMIR_06010

STRL:

HEP84_39535

AGY:	ATC03_10810

SALD:

FVA74_07450

LALL:

MUN78_00425 MUN78_00620

ART:

Arth_1727

ARY:	ATC04_12915

ACRY:

AC20117_03845 AC20117_16210

ASUF:

MNQ99_03120 MNQ99_13155

APOA:

J0916_03275

ACAO:

NF551_15045

ARTQ:

CGQ24_02105

ARZ:	AUT26_19685

POK:	SMD14_08735

GLU:	F0M17_06450

GPR:	JQN66_15940

GMY:

XH9_02265

GNC:	QQS42_06460

CCYC:

SCMU_02260

CMUA:

P8192_13935

BLUT:

EW640_11445

NOCW:

ABFU82_18940

PSIM:

KR76_01035

THAO:

NI17_008420

ACTW:

F7P10_09220

AMAZ:

LUW76_05590 LUW76_06985

BSD:	BLASA_2994

BBRG:

U6N30_15690

BMON:

ABDB74_10835

MMAR:

MODMU_2722

AMQ:	AMETH_2741(cypA)

AMYY:

YIM_24070(vdh1)

AORI:

SD37_05135

AACD:

LWP59_14425

APRT:

MUY14_09310

ATHM:

L1857_16525

ARHD:

VSH64_04160 VSH64_39570

ACAB:

QRX50_05515

ANW:	QP939_31980

AMYD:

K1T34_20635

ABAR:

AMYBAR_002555

PDX:

Psed_3265

PSEA:

WY02_26855

PSEH:

XF36_15935

PSEQ:

AD006_30685

PECQ:

AD017_30555

PAUT:

Pdca_32280

PBRO:

HOP40_06445 HOP40_08630 HOP40_32540

PPEL:

H6H00_24725

PSDS:

K1T35_35345

SESP:

BN6_55130

ACTY:

OG774_04290 OG774_28210

AMS:

AMIS_8190

ACTE:

ACTI_50060

EKE:	EK0264_12805

BALA:

DSM104299_02021

» show all

Reference

1 [PMID:188654]

Authors

Bernhardt FH, Nastainczyk W, Seydewitz V.

Title

Kinetic studies on a 4-methoxybenzoate O-demethylase from Pseudomonas putida.

Journal

Eur J Biochem 72:107-15 (1977)
DOI:10.1111/j.1432-1033.1977.tb11230.x

Reference

2 [PMID:25369]

Authors

Paszczynski A, Trojanowski J.

Title

An affinity-column procedure for the purification of veratrate O-demethylase from fungi.

Journal

Microbios 18:111-21 (1977)

Reference

3 [PMID:6273164]

Authors

Twilfer H, Bernhardt FH, Gersonde K.

Title

An electron-spin-resonance study on the redox-active centers of the 4-methoxybenzoate monooxygenase from Pseudomonas putida.

Journal

Eur J Biochem 119:595-602 (1981)
DOI:10.1111/j.1432-1033.1981.tb05649.x

Other DBs

ExplorEnz - The Enzyme Database:

1.14.99.15

IUBMB Enzyme Nomenclature:

1.14.99.15

ExPASy - ENZYME nomenclature database:

1.14.99.15

UM-BBD (Biocatalysis/Biodegradation Database):

1.14.99.15

BRENDA, the Enzyme Database:

1.14.99.15

CAS:	37256-78-3

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