KEGG   ENZYME: 1.14.99.22
Entry
EC 1.14.99.22               Enzyme                                 
Name
ecdysone 20-monooxygenase;
alpha-ecdysone C-20 hydroxylase;
ecdysone 20-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
Sysname
Ecdysone,hydrogen-donor:oxygen oxidoreductase (20-hydroxylating)
Reaction(IUBMB)
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O [RN:R02374]
Reaction(KEGG)
R02374
Substrate
ecdysone [CPD:C00477];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]
Product
20-hydroxyecdysone [CPD:C02633];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Comment
An enzyme from insect fat body or malpighian tubules involving a heme-thiolate protein (P-450). NADPH can act as ultimate hydrogen donor.
History
EC 1.14.99.22 created 1978
Pathway
ec00981  Insect hormone biosynthesis
Orthology
K10723  ecdysone 20-monooxygenase
Genes
DMEDmel_CG13478(shd)
DER6545923
DSE6605674
DSIDsimw501_GD14493(Dsim_GD14493)
DYADyak_GE22018
DSR110182778
DPO4813495
DPE6601974
DMN108153350
DWI6651961
DGR6557434
DMODmoj_GI11492
DAZ108613535
DNV108652348
DHE111599777
DVI6624357
CCAT101458671
BOD106620118
BDR105225924
RZE108376498
AOQ129242403
TDA119672901 119672949
MDE101896979
SCAC106094533
LCQ111681384
LSQ119602150
GFS119632129
ECOE129942871
CLON129609090
HIS119648671
AGA1273531
ACOZ120954001
AARA120898182
AMER121590616
ASTE118508360
AFUN125765021
AMOU128309666
AALI118461982
AAG110677901 5574128
AALB109413055 115261322
ASUA134219073
CQUCpipJ_CPIJ001380
CPII120426314
CNS116342612
BCOO119084419
AME411057(Cyp314a1)
ACER107998545
ALAB122715177
ADR102671230
AFLR100864138
BIM100742793
BBIF117205002
BVK117230953
BVAN117153185
BTER100649449
BAFF126921824
BPYO122567730
BPAS132912708
FVI122537775
CCAL108626703
OBB114878757
OLG117602960
MGEN117221401
NMEA116423841
CGIG122399165
SOC105204717
MPHA105834373
PBAR105423368
VEM105561274
HST105191056
DQU106752394
CFO105250706
FEX115239873
LHU105677150
PGC109859613
OBO105275798
PCF106787266
PFUC122525412
VPS122633621
VCRB124429609
VVE124953635
NVI100115247(CYP314A1)
CSOL105367236
TPRE106652308
LHT122513022
LBD127284988
MDL103568534
CGLO123259430
FAS105273657
DAM107037813
AGIF122847608
CINS118069741
VCAN122412462
CCIN107274383
DSM124408556
NPT124217999
NFB124179596
NLO107222708
NVG124303486
AROA105692836
TCA661451(Cyp314a1)
DPA109533342
SOY115879439
AGRG126748264
ATD109593984
CSET123318783
AGB108916084
LDC111509668
DVV114338208
NVL108563010
APLN108741669
PPYR116164624
OTU111428502
BMOR100127102(Cyp314a1)
BMAN114243638
MSEX115447334
DPLKGM_205009
BANY112056276
MJU123877070
NIQ126776248
VCD124538833
MCIX123664117
PMAC106715493
PPOT106104241
PXU106116734
PRAP110995369
PBX123710134
PNAP125053705
ZCE119828929
CCRC123700719
LSIN126970515
AAGE121736020
HAW110376828
HZE124640792
TNL113499203 113508193
SLIU111361821
OFU114364622
ATRA106131561
PXY105393180
PGW126379951
LGLY125232729
CFEL113367864
API100165833 100167431
DNX107173501
AGS114128007
RMD113549981
ACOO126839151
DVT126902450
BTAB109032884
DCI103524191
CLEC106668127
HHAL106689460
NLU111049897
HVI124360224
MQU128991930
PHUPhum_PHUM213010
FOC113205624
TPAL117649332
ZNE110832310
CSEC111867707
SGRE126299150
BROR134541660
IEL124169385
FCD110845053
DPXDAPPUDRAFT_198743
DMK116921838
DPZ124327113
HAME121873562
LSM121120678
TCF131890996
DSV119460544
RSAN119397008
RMP119174035
VDE111245503
VJA111271365
TUT107372108
DPTE113790380
DFR124492652
CSCU111627474
PTEP107453276
ABRU129972910
UDV129233134
SDM118190615
LPOL106473002
 » show all
Reference
1  [PMID:606249]
  Authors
Johnson P, Rees HH.
  Title
The mechanism of C-20 hydroxylation of alpha-ecdysone in the desert locust, Schistocerca gregaria.
  Journal
Biochem J 168:513-20 (1977)
DOI:10.1042/bj1680513
Reference
2  [PMID:5286]
  Authors
Nigg HN, Svoboda JA, Thompson MJ, Dutky SR, Kaplanis JN, Robbins WE.
  Title
Ecdysome 20-hydroxylase from the midgut of the tobacco hornworm (Manduca sexta L.).
  Journal
Experientia 32:438-9 (1976)
DOI:10.1007/BF01920781
Reference
3  [PMID:488526]
  Authors
Smith SL, Bollenbacher WE, Cooper DY, Schleyer H, Wielgus JJ, Gilbert LI.
  Title
Ecdysone 20-monooxygenase: characterization of an insect cytochrome p-450 dependent steroid hydroxylase.
  Journal
Mol Cell Endocrinol 15:111-33 (1979)
DOI:10.1016/0303-7207(79)90033-9
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.22
IUBMB Enzyme Nomenclature: 1.14.99.22
ExPASy - ENZYME nomenclature database: 1.14.99.22
BRENDA, the Enzyme Database: 1.14.99.22
CAS: 55071-97-1

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