KEGG   ENZYME: 1.14.99.38
Entry
EC 1.14.99.38               Enzyme                                 

Name
cholesterol 25-monooxygenase;
cholesterol 25-hydroxylase (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
Sysname
cholesterol,hydrogen-donor:oxygen oxidoreductase (25-hydroxylating)
Reaction(IUBMB)
cholesterol + reduced acceptor + O2 = 25-hydroxycholesterol + acceptor + H2O [RN:R07218]
Reaction(KEGG)
R07218
Substrate
cholesterol [CPD:C00187];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]
Product
25-hydroxycholesterol [CPD:C15519];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Comment
Unlike most other sterol hydroxylases, this enzyme is not a cytochrome P-450. Instead, it uses diiron cofactors to catalyse the hydroxylation of hydrophobic substrates [1]. The diiron cofactor can be either Fe-O-Fe or Fe-OH-Fe and is bound to the enzyme through interactions with clustered histidine or glutamate residues [4,5]. In cell cultures, this enzyme down-regulates cholesterol synthesis and the processing of sterol regulatory element binding proteins (SREBPs). cf. EC 1.17.99.10, cholesterol C-25 hydroxylase.
History
EC 1.14.99.38 created 2005, modified 2020
Pathway
ec00120  Primary bile acid biosynthesis
Orthology
K10223  cholesterol 25-hydroxylase
Genes
HSA: 9023(CH25H)
PTR: 450584(CH25H)
PPS: 100974384(CH25H)
GGO: 101125247(CH25H)
PON: 100452872(CH25H)
NLE: 100583426(CH25H)
MCC: 694532(CH25H)
MCF: 102142173(CH25H)
CSAB: 103216226(CH25H)
CATY: 105587154(CH25H)
PANU: 101025055(CH25H)
RRO: 104675146(CH25H)
RBB: 108526604(CH25H)
TFN: 117086228(CH25H)
PTEH: 111551335(CH25H)
CJC: 100405652(CH25H)
SBQ: 101034268(CH25H)
MMUR: 105871663(CH25H)
MMU: 12642(Ch25h)
MCAL: 110286100(Ch25h)
MPAH: 110336789(Ch25h)
RNO: 309527(Ch25h)
MCOC: 116101152(Ch25h)
MUN: 110565101(Ch25h)
CGE: 100762824(Ch25h)
NGI: 103747462(Ch25h)
HGL: 101713638(Ch25h)
CCAN: 109694732(Ch25h)
OCU: 100341039(CH25H)
TUP: 102477002(CH25H)
CFA: 100856263(CH25H)
VVP: 112931975(CH25H)
VLG: 121475644(CH25H)
AML: 100470751(CH25H)
UMR: 103661935(CH25H)
UAH: 113258967(CH25H)
ORO: 101385951(CH25H)
ELK: 111145154
MPUF: 101677681(CH25H)
EJU: 114210228(CH25H)
MLX: 118012957(CH25H)
FCA: 101098893(CH25H)
PPAD: 109255418(CH25H)
AJU: 113596491(CH25H)
HHV: 120223792(CH25H)
BTA: 506132(CH25H)
BOM: 102271621(CH25H)
BIU: 109553211(CH25H)
BBUB: 102409279(CH25H)
CHX: 102191759(CH25H)
OAS: 101121645(CH25H)
ODA: 120873795(CH25H)
SSC: 100157868(CH25H)
CFR: 102516799(CH25H)
CBAI: 105081851(CH25H)
CDK: 105102833(CH25H)
BACU: 103019491(CH25H)
LVE: 103080660(CH25H)
OOR: 101271130(CH25H)
DLE: 111172517(CH25H)
PCAD: 102984806(CH25H)
ECB: 100062138(CH25H)
EPZ: 103566587(CH25H)
EAI: 106831912(CH25H)
MYB: 102240160(CH25H)
MYD: 102760598(CH25H)
MMYO: 118669988(CH25H)
MNA: 107543291(CH25H)
HAI: 109377127(CH25H)
DRO: 112307321(CH25H)
SHON: 118987957(CH25H)
AJM: 119057716 119057746(CH25H)
MMF: 118632719(CH25H)
PALE: 102879471(CH25H)
PGIG: 120609344(CH25H)
RAY: 107502210(CH25H)
MJV: 108392866(CH25H)
TOD: 119233913(CH25H)
LAV: 100657141(CH25H)
TMU: 101357853
SHR: 100930151(CH25H)
PCW: 110198978(CH25H)
OAA: 100078582(CH25H)
GGA: 423788(CH25H)
PCOC: 116238153(CH25H)
MGP: 100550290(CH25H)
CJO: 107315869(CH25H)
NMEL: 110399914(CH25H)
APLA: 101794901(CH25H)
ACYG: 106044273(CH25H)
TGU: 100222744(CH25H)
LSR: 110475749(CH25H)
SCAN: 103813932(CH25H)
PMOA: 120502037(CH25H)
GFR: 102033532(CH25H)
FAB: 101806492(CH25H)
PHI: 102102616(CH25H) 102109333
PMAJ: 107206999(CH25H)
CCAE: 111931167(CH25H)
CCW: 104696497(CH25H)
ETL: 114055897(CH25H)
FPG: 101915580(CH25H)
FCH: 102060118(CH25H)
CLV: 102084518(CH25H)
EGZ: 104129156(CH25H)
NNI: 104013120(CH25H)
ACUN: 113481803(CH25H)
PADL: 103913652(CH25H)
AAM: 106487843(CH25H)
ASN: 102371917(CH25H)
AMJ: 102561874(CH25H)
CPOO: 109324314(CH25H)
GGN: 109287470(CH25H)
PSS: 102456208(CH25H)
CMY: 102939174(CH25H)
CPIC: 101938379(CH25H)
TST: 117880266(CH25H)
CABI: 116818987(CH25H)
ACS: 100554078 100568259(ch25h)
PVT: 110070876(CH25H)
PBI: 103058588(CH25H)
PMUR: 107293838(CH25H)
TSR: 106548506(CH25H)
PGUT: 117673144(CH25H)
PMUA: 114596710(CH25H)
ZVI: 118096366(CH25H)
GJA: 107121654(CH25H)
XLA: 100037239(ch25h.L) 108697353(ch25h.S) 108710600
DRE: 100005337 100136851(ch25hl2) 336673(ch25hl1.1) 494109(ch25h) 798384(ch25hl1.2)
IPU: 100528885(ch25h) 108255855 108278808(ch25hl2)
ELY: 117246843 117248764 117251940(ch25hl3) 117262752(ch25hl1.1) 117262835(ch25hl1.2) 117270128(ch25hl2)
PLEP: 121944851(ch25hl3) 121950428(ch25hl1.2) 121950544(ch25hl1.1) 121958812(ch25h) 121959425(ch25hl2)
SLUC: 116038559(ch25hl1.2) 116050499(ch25hl3) 116058002(ch25h) 116062991(ch25hl2)
ECRA: 117940092 117944316(ch25hl3) 117949050(ch25hl1.1) 117959182(ch25hl2)
GAT: 120809648(ch25hl1.1) 120812854 120819662(ch25h) 120830874 120831757(ch25hl2)
MSAM: 119892469 119893763(ch25hl1.1) 119894120(ch25hl1.2) 119896308 119904787 119914596(ch25hl2) 119915784(ch25h) 119916808
CUD: 121509691(ch25hl3) 121514558(ch25hl1.2) 121515688(ch25hl1.1) 121525603(ch25hl2) 121528501(ch25h)
OAU: 116315924(ch25hl2) 116322653(ch25hl3) 116324416(ch25h) 116326256(ch25hl1.1) 116326257(ch25hl1.2)
OML: 112141841(ch25h) 112147991(ch25hl3) 112151827(ch25hl1.2) 112152648(ch25hl1.1) 112162812(ch25hl2)
CTUL: 119772477(ch25hl1.1) 119772481 119788292(ch25hl2) 119792881(ch25h) 119795570(ch25hl3)
KMR: 108229945(ch25hl1.1) 108230007(ch25hl1.2) 108234932(ch25hl3) 108244749(ch25hl2) 108250736(ch25h)
XGL: 120792869(ch25hl1.2) 120793386(ch25hl1.1) 120793946(ch25h) 120796129 120805429(ch25hl3) 120806724(ch25hl2)
AANG: 118213427(ch25hl2) 118215571(ch25hl1.2) 118221090(ch25h) 118222843(ch25hl1.1) 118237105(ch25hl3)
CIN: 104266587
SPU: 583592
CEL: CELE_F35C8.5(F35C8.5)
CBR: CBG14675
ADF: 107342391
AMIL: 114976650
 » show all
Reference
1  [PMID:9852097]
  Authors
Lund EG, Kerr TA, Sakai J, Li WP, Russell DW
  Title
cDNA cloning of mouse and human cholesterol 25-hydroxylases, polytopic membrane proteins that synthesize a potent oxysterol regulator of lipid metabolism.
  Journal
J Biol Chem 273:34316-27 (1998)
DOI:10.1074/jbc.273.51.34316
  Sequence
[hsa:9023] [mmu:12642]
Reference
2  [PMID:11967195]
  Authors
Chen JJ, Lukyanenko Y, Hutson JC.
  Title
25-hydroxycholesterol is produced by testicular macrophages during the early postnatal period and influences differentiation of Leydig cells in vitro.
  Journal
Biol Reprod 66:1336-41 (2002)
DOI:10.1095/biolreprod66.5.1336
  Sequence
[rno:309527]
Reference
3  [PMID:12390873]
  Authors
Lukyanenko Y, Chen JJ, Hutson JC.
  Title
Testosterone regulates 25-hydroxycholesterol production in testicular macrophages.
  Journal
Biol Reprod 67:1435-8 (2002)
DOI:10.1095/biolreprod.102.007575
  Sequence
[rno:309527]
Reference
4  [PMID:7947683]
  Authors
Fox BG, Shanklin J, Ai J, Loehr TM, Sanders-Loehr J.
  Title
Resonance Raman evidence for an Fe-O-Fe center in stearoyl-ACP desaturase. Primary sequence identity with other diiron-oxo proteins.
  Journal
Biochemistry 33:12776-86 (1994)
DOI:10.1021/bi00209a008
Reference
5  [PMID:12543708]
  Authors
Russell DW
  Title
The enzymes, regulation, and genetics of bile acid synthesis.
  Journal
Annu Rev Biochem 72:137-74 (2003)
DOI:10.1146/annurev.biochem.72.121801.161712
  Sequence
[mmu:12642]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.38
IUBMB Enzyme Nomenclature: 1.14.99.38
ExPASy - ENZYME nomenclature database: 1.14.99.38
BRENDA, the Enzyme Database: 1.14.99.38
CAS: 60202-07-5

DBGET integrated database retrieval system