KEGG   ENZYME: 1.14.99.48
Entry
EC 1.14.99.48               Enzyme                                 

Name
heme oxygenase (staphylobilin-producing);
haem oxygenase (ambiguous);
heme oxygenase (decyclizing) (ambiguous);
heme oxidase (ambiguous);
haem oxidase (ambiguous);
heme oxygenase (ambiguous);
isdG (gene name);
isdI (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
Sysname
protoheme,hydrogen-donor:oxygen oxidoreductase (delta/beta-methene-oxidizing, hydroxylating)
Reaction(IUBMB)
(1) protoheme + 5 reduced acceptor + 4 O2 = beta-staphylobilin + Fe2+ + formaldehyde + 5 acceptor + 4 H2O [RN:R10468];
(2) protoheme + 5 reduced acceptor + 4 O2 = delta-staphylobilin + Fe2+ + formaldehyde + 5 acceptor + 4 H2O [RN:R10510]
Reaction(KEGG)
R10468 R10510
Substrate
protoheme [CPD:C00032];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]
Product
beta-staphylobilin [CPD:C20667];
Fe2+ [CPD:C14818];
formaldehyde [CPD:C00067];
acceptor [CPD:C00028];
H2O [CPD:C00001];
delta-staphylobilin [CPD:C20666]
Comment
This enzyme, which is found in some pathogenic bacteria, is involved in an iron acquisition system that catabolizes the host's hemoglobin. The two enzymes from the bacterium Staphylococcus aureus, encoded by the isdG and isdI genes, produce 67.5 % and 56.2 % delta-staphylobilin, respectively.
History
EC 1.14.99.48 created 2013
Pathway
ec00860  Porphyrin and chlorophyll metabolism
ec01110  Biosynthesis of secondary metabolites
Orthology
K07145  heme oxygenase (staphylobilin-producing)
Genes
VIT: ADP71_24520
DALK: DSCA_15890 DSCA_41270
SDO: SD1155_00595
BSU: BSU07150(yetG)
BSR: I33_0806
BSL: A7A1_1759
BSH: BSU6051_07150(yetG)
BSY: I653_03610
BSUT: BSUB_00788(hmoA)
BSUL: BSUA_00788(hmoA)
BSUS: Q433_04020
BSS: BSUW23_03645(yetG)
BST: GYO_0975
BSQ: B657_07150(hmoA)
BSX: C663_0743(yetG)
BAQ: BACAU_0699(yetG)
BYA: BANAU_0651(yetG)
BAMP: B938_03450
BAML: BAM5036_0649(hmoA)
BAMA: RBAU_0713(hmoA)
BAMN: BASU_0690(hmoA)
BAMB: BAPNAU_0661(yetG)
BAMT: AJ82_04065
BAMY: V529_06760(yetG)
BMP: NG74_00725(hmoA)
BAO: BAMF_0686(yetG)
BAZ: BAMTA208_03235(yetG)
BQL: LL3_00735(yetG)
BXH: BAXH7_00680(yetG)
BQY: MUS_0716(yetG)
BAMI: KSO_016125
BAMC: U471_07060
BAMF: U722_03630
BSON: S101395_01740(isdG)
BAN: BA_2987 BA_4782(isdG)
BAI: BAA_3041 BAA_4795(isdG)
BANV: DJ46_1749 DJ46_3451(isdG)
BCQ: BCQ_2805(yetG) BCQ_4348
BCX: BCA_3058 BCA_4648(isdG)
BTM: MC28_2172 MC28_3822(isdG)
BTG: BTB_c30980(yetG) BTB_c46910(isdG)
BTW: BF38_295(isdG) BF38_4139
BWW: bwei_0354(isdG) bwei_2040
BMYO: BG05_1501(isdG) BG05_3039
BMYC: DJ92_71
BAG: Bcoa_2560
BJS: MY9_0807
BACP: SB24_06200
BACB: OY17_06315
BACO: OXB_2435
BACY: QF06_02480
BACL: BS34A_08140(yetG)
BALM: BsLM_0754
BBEV: BBEV_2100
BKW: BkAM31D_25155(hmoA)
AFL: Aflv_2491
HLI: HLI_17115
VPN: A21D_01200(isdG)
PASA: BAOM_5089(isdG)
BSE: Bsel_2560
SAU: SA0160 SA0983(isdG)
SAM: MW0140 MW1018(isdG)
SAX: USA300HOU_0179(isdG1) USA300HOU_1069(isdG2)
SAE: NWMN_0111 NWMN_1047(isdG)
SUU: M013TW_0155(isdI) M013TW_1069(isdG)
SUJ: SAA6159_00149(isdI) SAA6159_00990(isdG)
SUK: SAA6008_00143(isdI) SAA6008_01090(isdG)
SUZ: MS7_0159(isdI) MS7_1092(isdG)
SUW: SATW20_01760(isdI) SATW20_11300(isdG)
SAUE: RSAU_000119(isdI) RSAU_001020(isdG)
SAUS: SA40_0132(isdI) SA40_1006(isdG)
SAUU: SA957_0147(isdI) SA957_1021(isdG)
SAUG: SA268_0144(isdI) SA268_1024(isdG)
SAUF: X998_0148(isdI) X998_1133(isdG)
SAB: SAB1000
SUY: SA2981_0166(isdI) SA2981_1092(isdG)
SAUC: CA347_1050(isdI) CA347_177(isdI)
SAUR: SABB_00362(isdG) SABB_01676(isdI)
SER: SERP1773
SEP: SE1764
SEPP: SEB_01773
SEPS: DP17_717
SHA: SH0867
SHH: ShL2_00760(isdI)
SSP: SSP0713
SLN: SLUG_01000(isdG)
SDT: SPSE_0265(isdI)
SPAS: STP1_0602(isdG)
SXO: SXYL_00755(isdG)
SHU: SHYC_11695(isdI)
SCAP: AYP1020_0461(isdG) AYP1020_1366(isdI)
SSCH: LH95_01615
SSCZ: RN70_01955
SAGQ: EP23_10475
SDP: NCTC12225_00319(isdI)
SARL: SAP2_07790(isdG)
MCL: MCCL_0304
MCAK: MCCS_04680(isdG)
LMO: lmo0484
LMOE: BN418_0554
LMOB: BN419_0559
LMOD: LMON_0484
LMOW: AX10_10940
LMOQ: LM6179_0783(isdG)
LMR: LMR479A_0491(isdG)
LMOM: IJ09_07955
LMOG: BN389_05190(isdG)
LMP: MUO_02665
LMOZ: LM1816_10672(isdG)
LMOX: AX24_15315
LMQ: LMM7_0504(isdG)
LMS: LMLG_2751
LMOK: CQ02_02645
LMV: Y193_13320(isdG)
LIN: lin0487
LWE: lwe0452
LSG: lse_0393
LIV: LIV_0383
LGZ: NCTC10812_00640(isdG)
BLR: BRLA_c025910(hmoA)
PPY: PPE_04042
PPM: PPSC2_21345(isdG)
PPO: PPM_4254(isdG)
PPOL: X809_37370
PPOY: RE92_15910
PMW: B2K_36325
PSAB: PSAB_22785
PRI: PRIO_6394
PSWU: SY83_12315
KZO: NCTC404_00637(isdI_1) NCTC404_02707(isdG) NCTC404_03056(isdI_2)
CML: BN424_3514(isdG)
ERH: ERH_0453
ACL: ACL_0959
ABRA: BN85311450
APAL: BN85405740
AAXA: NCTC10138_01404(isdG)
SFK: KY5_6671c
TRA: Trad_0114
PSAC: PSM36_1783
FIN: KQS_08855(fjo15)
HTU: Htur_4641
 » show all
Reference
1  [PMID:20180905]
  Authors
Reniere ML, Ukpabi GN, Harry SR, Stec DF, Krull R, Wright DW, Bachmann BO, Murphy ME, Skaar EP
  Title
The IsdG-family of haem oxygenases degrades haem to a novel chromophore.
  Journal
Mol Microbiol 75:1529-38 (2010)
DOI:10.1111/j.1365-2958.2010.07076.x
  Sequence
[sau:SA0160]
Reference
2  [PMID:23600533]
  Authors
Matsui T, Nambu S, Ono Y, Goulding CW, Tsumoto K, Ikeda-Saito M
  Title
Heme degradation by Staphylococcus aureus IsdG and IsdI liberates formaldehyde rather than carbon monoxide.
  Journal
Biochemistry 52:3025-7 (2013)
DOI:10.1021/bi400382p
Reference
3  [PMID:26534961]
  Authors
Streit BR, Kant R, Tokmina-Lukaszewska M, Celis AI, Machovina MM, Skaar EP, Bothner B, DuBois JL
  Title
Time-resolved Studies of IsdG Protein Identify Molecular Signposts along the Non-canonical Heme Oxygenase Pathway.
  Journal
J Biol Chem 291:862-71 (2016)
DOI:10.1074/jbc.M115.666560
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.48
IUBMB Enzyme Nomenclature: 1.14.99.48
ExPASy - ENZYME nomenclature database: 1.14.99.48
BRENDA, the Enzyme Database: 1.14.99.48

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