KEGG   ENZYME: 1.14.99.54
Entry
EC 1.14.99.54               Enzyme                                 
Name
lytic cellulose monooxygenase (C1-hydroxylating);
lytic polysaccharide monooxygenase (ambiguous);
LPMO (ambiguous);
LPMO9A
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
Sysname
cellulose, hydrogen-donor:oxygen oxidoreductase (D-glucosyl C1-hydroxylating)
Reaction(IUBMB)
[(1->4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = [(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone + [(1->4)-beta-D-glucosyl]n + acceptor + H2O [RN:R11745]
Reaction(KEGG)
R11745
Substrate
[(1->4)-beta-D-glucosyl]n+m;
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]
Product
[(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone;
[(1->4)-beta-D-glucosyl]n;
acceptor [CPD:C00028];
H2O [CPD:C00001]
Comment
This copper-containing enzyme, found in fungi and bacteria, cleaves cellulose in an oxidative manner. The cellulose fragments that are formed contain a D-glucono-1,5-lactone residue at the reducing end, which hydrolyses quickly and spontaneously to the aldonic acid. The electrons are provided in vivo by the cytochrome b domain of EC 1.1.99.18, cellobiose dehydrogenase (acceptor) [1]. Ascorbate can serve as the electron donor in vitro.
History
EC 1.14.99.54 created 2017
Orthology
K19356  lytic cellulose monooxygenase (C1-hydroxylating)
Genes
NCRNCU01867(gh61-10) NCU02240(gh61-1) NCU02916(gh61-3) NCU07760(gh61-2) NCU08760(gh61-5)
NTENEUTE1DRAFT133749(NEUTE1DRAFT_133749) NEUTE1DRAFT77711(NEUTE1DRAFT_77711) NEUTE1DRAFT82948(NEUTE1DRAFT_82948) NEUTE1DRAFT92381(NEUTE1DRAFT_92381)
SMPSMAC_00529 SMAC_03679 SMAC_04518 SMAC_05160 SMAC_06537 SMAC_06847
PANPODANSg1734 PODANSg1847 PODANSg2226 PODANSg3538 PODANSg3552 PODANSg4671 PODANSg4737 PODANSg716 PODANSg7957 PODANSg8471
TTTTHITE_2106556 THITE_2119040 THITE_2142696 THITE_2170662
MTMMYCTH_111088 MYCTH_2299721 MYCTH_2301632 MYCTH_46583 MYCTH_47093 MYCTH_79765 MYCTH_80312
CTHRCTHT_0000930 CTHT_0022130 CTHT_0025030 CTHT_0052310 CTHT_0072140
MGRMGG_07300 MGG_08020 MGG_12733 MGG_13241
PPEIPpBr36_01463 PpBr36_01943 PpBr36_02795 PpBr36_06416
PGRIPgNI_00792 PgNI_01920 PgNI_03554 PgNI_05405 PgNI_07727
TMNUCRPA7_2324 UCRPA7_5174 UCRPA7_5653 UCRPA7_5990
SSCKSPSK_01239 SPSK_01887 SPSK_04252 SPSK_09715
FGRFGSG_03695 FGSG_04681 FGSG_04773 FGSG_06110 FGSG_06397 FGSG_13245
FPUFPSE_00868 FPSE_01837 FPSE_02468 FPSE_04448 FPSE_07781 FPSE_12300
FPOAFPOAC1_006095 FPOAC1_009394 FPOAC1_011706
FVNFVRRES_06695 FVRRES_09706 FVRRES_10031 FVRRES_12762
FVRFVEG_03506 FVEG_04743 FVEG_06459 FVEG_08783 FVEG_12089 FVEG_13504
FOXFOXG_03771 FOXG_05654 FOXG_07821 FOXG_08211 FOXG_09688
NHENECHADRAFT_100325 NECHADRAFT_106448 NECHADRAFT_19032 NECHADRAFT_33435 NECHADRAFT_54993 NECHADRAFT_96284
FFCNCS54_00283300 NCS54_00498700 NCS54_01155600 NCS54_01304900
FMUJ7337_004332 J7337_006778 J7337_007216 J7337_012544
TRETRIREDRAFT_120961(cel61b) TRIREDRAFT_73643(cel61a)
TRRM419DRAFT_122518 M419DRAFT_139633
PCHMVFPPC_05993
VALVDBG_01593 VDBG_02240 VDBG_03308 VDBG_06492 VDBG_07467 VDBG_08426 VDBG_09242 VDBG_09562
VDAVDAG_01959 VDAG_02211 VDAG_02299 VDAG_02711 VDAG_02959 VDAG_03411 VDAG_04756 VDAG_04795 VDAG_05030 VDAG_07400 VDAG_08091
CFJCFIO01_02234 CFIO01_03891 CFIO01_04309 CFIO01_04911 CFIO01_05451 CFIO01_05808 CFIO01_08914 CFIO01_10036 CFIO01_10898
CLUPCLUP02_04841 CLUP02_07184 CLUP02_11571 CLUP02_11778 CLUP02_16314 CLUP02_16898
CHIGCH63R_00346 CH63R_03679 CH63R_06721 CH63R_09615 CH63R_10341 CH63R_10722 CH63R_11040 CH63R_11265 CH63R_12595
SAPOSAPIO_CDS10182 SAPIO_CDS1237 SAPIO_CDS1238 SAPIO_CDS1536 SAPIO_CDS6033 SAPIO_CDS6278 SAPIO_CDS8458 SAPIO_CDS9232
ELAUCREL1_10876 UCREL1_1520 UCREL1_2340 UCREL1_312 UCREL1_319 UCREL1_3268 UCREL1_3417 UCREL1_3819 UCREL1_4406 UCREL1_4875 UCREL1_5189 UCREL1_6396 UCREL1_6408 UCREL1_9067
PFYPFICI_01254 PFICI_01743 PFICI_03695 PFICI_04173 PFICI_08510 PFICI_10057 PFICI_12594 PFICI_14484 PFICI_15159 PFICI_15210
SSLSS1G_01838 SS1G_09251 SS1G_12059
BFUBCIN_02g02040 BCIN_03g05890 BCIN_05g08230 BCIN_06g00480
MBEMBM_00712 MBM_04929 MBM_05468 MBM_06977
PSCOLY89DRAFT_315829 LY89DRAFT_582205 LY89DRAFT_632963 LY89DRAFT_634019 LY89DRAFT_760760 LY89DRAFT_772659
GLZGLAREA_00867 GLAREA_02347 GLAREA_03944 GLAREA_04229 GLAREA_08312 GLAREA_08367 GLAREA_09224 GLAREA_11571 GLAREA_12658
ANIAN1602.2 AN9524.2
AFMAFUA_3G03870 AFUA_8G06830
ACTACLA_059790
NFINFIA_044390 NFIA_099510
AORAO090005000531
ANGAn14g02670 An15g04900
AFVAFLA_003339
ALUCAKAW2_40943A AKAW2_60257S AKAW2_60853S
APUUAPUU_10910S APUU_21609A APUU_80927A
PCSN7525_001242
POUPOX_g09366
PNOSNOG_00879 SNOG_01146 SNOG_01922 SNOG_05441 SNOG_05737 SNOG_05750 SNOG_07503 SNOG_10071 SNOG_11619 SNOG_12037 SNOG_14481
PTEPTT_04452 PTT_08333 PTT_09156 PTT_10713 PTT_12296 PTT_12378 PTT_12389 PTT_12419 PTT_13147 PTT_14450 PTT_19839
BZECOCCADRAFT_10290 COCCADRAFT_27639 COCCADRAFT_37868 COCCADRAFT_80996 COCCADRAFT_81058 COCCADRAFT_82910 COCCADRAFT_8654 COCCADRAFT_88798 COCCADRAFT_89549 COCCADRAFT_90790 COCCADRAFT_91341 COCCADRAFT_97778
BSCCOCSADRAFT_141473 COCSADRAFT_163931 COCSADRAFT_173335 COCSADRAFT_184615 COCSADRAFT_217864 COCSADRAFT_296363 COCSADRAFT_32038 COCSADRAFT_33583 COCSADRAFT_33682 COCSADRAFT_34597 COCSADRAFT_36666 COCSADRAFT_41104
BORCOCMIDRAFT_100168 COCMIDRAFT_102803 COCMIDRAFT_24605 COCMIDRAFT_25375 COCMIDRAFT_35579 COCMIDRAFT_36538 COCMIDRAFT_36916 COCMIDRAFT_5637 COCMIDRAFT_85853 COCMIDRAFT_87449 COCMIDRAFT_895 COCMIDRAFT_90535 COCMIDRAFT_99299
AALTCC77DRAFT_1005473 CC77DRAFT_1006432 CC77DRAFT_1019743 CC77DRAFT_1028092 CC77DRAFT_1044591 CC77DRAFT_1056084 CC77DRAFT_1057322 CC77DRAFT_190672 CC77DRAFT_307404 CC77DRAFT_504833 CC77DRAFT_705864 CC77DRAFT_937160 CC77DRAFT_940430 CC77DRAFT_941177 CC77DRAFT_948935
ARABEKO05_0003290 EKO05_0003463 EKO05_0006168 EKO05_0008432 EKO05_0008862 EKO05_0009560 EKO05_0009638 EKO05_0010429
NPAUCRNP2_10454 UCRNP2_3883 UCRNP2_5067
TMLGSTUM_00001211001
TVSTRAVEDRAFT_143379 TRAVEDRAFT_147827 TRAVEDRAFT_162601 TRAVEDRAFT_51004 TRAVEDRAFT_71674
DSQDICSQDRAFT_102982 DICSQDRAFT_104462 DICSQDRAFT_137375 DICSQDRAFT_181042
PCOPHACADRAFT_251256 PHACADRAFT_253391 PHACADRAFT_261448 PHACADRAFT_263097 PHACADRAFT_57963
SHSSTEHIDRAFT_105725 STEHIDRAFT_121343 STEHIDRAFT_98247
HIRHETIRDRAFT_63659(GH61H) HETIRDRAFT_64994(GH61I) HETIRDRAFT_67582(GH61D)
PSQPUNSTDRAFT_142953 PUNSTDRAFT_154782 PUNSTDRAFT_154784 PUNSTDRAFT_154785 PUNSTDRAFT_96583
ADLAURDEDRAFT_132370 AURDEDRAFT_140513 AURDEDRAFT_143034 AURDEDRAFT_165835 AURDEDRAFT_199593 AURDEDRAFT_86786
FMEFOMMEDRAFT_136574 FOMMEDRAFT_170904 FOMMEDRAFT_187182 FOMMEDRAFT_38849
RSXRhiXN_04074 RhiXN_05310 RhiXN_07972 RhiXN_07974 RhiXN_09310 RhiXN_09321 RhiXN_09329 RhiXN_09382 RhiXN_11095 RhiXN_12126
CCICC1G_02189 CC1G_03525 CC1G_03526 CC1G_03529 CC1G_04917 CC1G_07179 CC1G_07480 CC1G_09526 CC1G_09949 CC1G_10029 CC1G_13046
ABPAGABI1DRAFT111761(AGABI1DRAFT_111761) AGABI1DRAFT113096(AGABI1DRAFT_113096) AGABI1DRAFT80328(AGABI1DRAFT_80328)
ABVAGABI2DRAFT122757(AGABI2DRAFT_122757) AGABI2DRAFT192993(AGABI2DRAFT_192993) AGABI2DRAFT226235(AGABI2DRAFT_226235)
PCUBJR316_0002821 JR316_0007184 JR316_0008092 JR316_0011395 JR316_0011805 JR316_0011856
MPRMPER_08960 MPER_10540
MRRMoror_11015 Moror_12493 Moror_12809 Moror_14820 Moror_16033 Moror_16034
MOREE1B28_004841 E1B28_005688 E1B28_008037 E1B28_008051 E1B28_009655(CEL1) E1B28_013585 E1B28_013836
SCMSCHCO_01280218(SCHCODRAFT_01280218) SCHCO_02076751(SCHCODRAFT_02076751) SCHCO_02621099(SCHCODRAFT_02621099) SCHCO_02640531(SCHCODRAFT_02640531)
CPUTCONPUDRAFT_140516 CONPUDRAFT_91873
SLASERLADRAFT_335267
WSEWALSEDRAFT_32834
WICJ056_000100 J056_002693
 » show all
Reference
1  [PMID:22004347]
  Authors
Phillips CM, Beeson WT, Cate JH, Marletta MA
  Title
Cellobiose dehydrogenase and a copper-dependent polysaccharide monooxygenase potentiate cellulose degradation by Neurospora crassa.
  Journal
ACS Chem Biol 6:1399-406 (2011)
DOI:10.1021/cb200351y
  Sequence
[ncr:NCU08760]
Reference
2  [PMID:22188218]
  Authors
Beeson WT, Phillips CM, Cate JH, Marletta MA
  Title
Oxidative cleavage of cellulose by fungal copper-dependent polysaccharide monooxygenases.
  Journal
J Am Chem Soc 134:890-2 (2012)
DOI:10.1021/ja210657t
  Sequence
[ncr:NCU08760]
Reference
3  [PMID:22578542]
  Authors
Li X, Beeson WT 4th, Phillips CM, Marletta MA, Cate JH
  Title
Structural basis for substrate targeting and catalysis by fungal polysaccharide monooxygenases.
  Journal
Structure 20:1051-61 (2012)
DOI:10.1016/j.str.2012.04.002
Reference
4  [PMID:23124232]
  Authors
Bey M, Zhou S, Poidevin L, Henrissat B, Coutinho PM, Berrin JG, Sigoillot JC
  Title
Cello-oligosaccharide oxidation reveals differences between two lytic polysaccharide monooxygenases (family GH61) from Podospora anserina.
  Journal
Appl Environ Microbiol 79:488-96 (2013)
DOI:10.1128/AEM.02942-12
  Sequence
Reference
5  [PMID:26185526]
  Authors
Frommhagen M, Sforza S, Westphal AH, Visser J, Hinz SW, Koetsier MJ, van Berkel WJ, Gruppen H, Kabel MA
  Title
Discovery of the combined oxidative cleavage of plant xylan and cellulose by a new fungal polysaccharide monooxygenase.
  Journal
Biotechnol Biofuels 8:101 (2015)
DOI:10.1186/s13068-015-0284-1
Reference
6  [PMID:27213015]
  Authors
Patel I, Kracher D, Ma S, Garajova S, Haon M, Faulds CB, Berrin JG, Ludwig R, Record E
  Title
Salt-responsive lytic polysaccharide monooxygenases from the mangrove fungus Pestalotiopsis sp. NCi6.
  Journal
Biotechnol Biofuels 9:108 (2016)
DOI:10.1186/s13068-016-0520-3
Reference
7  [PMID:27152023]
  Authors
Courtade G, Wimmer R, Rohr AK, Preims M, Felice AK, Dimarogona M, Vaaje-Kolstad G, Sorlie M, Sandgren M, Ludwig R, Eijsink VG, Aachmann FL
  Title
Interactions of a fungal lytic polysaccharide monooxygenase with beta-glucan substrates and cellobiose dehydrogenase.
  Journal
Proc Natl Acad Sci U S A 113:5922-7 (2016)
DOI:10.1073/pnas.1602566113
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.54
IUBMB Enzyme Nomenclature: 1.14.99.54
ExPASy - ENZYME nomenclature database: 1.14.99.54
BRENDA, the Enzyme Database: 1.14.99.54

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