KEGG   ENZYME: 1.14.99.54Help
Entry
EC 1.14.99.54               Enzyme                                 

Name
lytic cellulose monooxygenase (C1-hydroxylating);
lytic polysaccharide monooxygenase (ambiguous);
LPMO (ambiguous);
LPMO9A
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
BRITE hierarchy
Sysname
cellulose, hydrogen-donor:oxygen oxidoreductase (D-glucosyl C1-hydroxylating)
Reaction(IUBMB)
[(1->4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = [(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone + [(1->4)-beta-D-glucosyl]n + acceptor + H2O [RN:R11745]
Reaction(KEGG)
Substrate
[(1->4)-beta-D-glucosyl]n+m;
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]
Product
[(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone;
[(1->4)-beta-D-glucosyl]n;
acceptor [CPD:C00028];
H2O [CPD:C00001]
Comment
This copper-containing enzyme, found in fungi and bacteria, cleaves cellulose in an oxidative manner. The cellulose fragments that are formed contain a D-glucono-1,5-lactone residue at the reducing end, which hydrolyses quickly and spontaneously to the aldonic acid. The electrons are provided in vivo by the cytochrome b domain of EC 1.1.99.18, cellobiose dehydrogenase (acceptor) [1]. Ascorbate can serve as the electron donor in vitro.
History
EC 1.14.99.54 created 2017
Orthology
K19356  lytic cellulose monooxygenase (C1-hydroxylating)
Genes
NCR: NCU01867(gh61-10) NCU02240(gh61-1) NCU02916(gh61-3) NCU07760(gh61-2) NCU08760(gh61-5)
NTE: NEUTE1DRAFT133749(NEUTE1DRAFT_133749) NEUTE1DRAFT77711(NEUTE1DRAFT_77711) NEUTE1DRAFT82948(NEUTE1DRAFT_82948) NEUTE1DRAFT92381(NEUTE1DRAFT_92381)
SMP: SMAC_00529 SMAC_03679 SMAC_04518 SMAC_05160 SMAC_06537 SMAC_06847
PAN: PODANSg1734 PODANSg1847 PODANSg2226 PODANSg3538 PODANSg3552 PODANSg4671 PODANSg4737 PODANSg716 PODANSg7957 PODANSg8471
TTT: THITE_2106556 THITE_2119040 THITE_2142696 THITE_2170662
MTM: MYCTH_111088 MYCTH_2299721 MYCTH_2301632 MYCTH_46583 MYCTH_79765 MYCTH_80312
CTHR: CTHT_0000930 CTHT_0022130 CTHT_0025030 CTHT_0052310 CTHT_0072140
MGR: MGG_07300 MGG_08020 MGG_12733 MGG_13241
TMN: UCRPA7_2324 UCRPA7_5174 UCRPA7_5653
SSCK: SPSK_01239 SPSK_01887 SPSK_02603 SPSK_04252 SPSK_09715
TRE: TRIREDRAFT_73643(cel61a)
ANI: AN1602.2
ANG: ANI_1_1540134(An15g04900)
ABP: AGABI1DRAFT111761(AGABI1DRAFT_111761) AGABI1DRAFT113096(AGABI1DRAFT_113096)
ABV: AGABI2DRAFT122757(AGABI2DRAFT_122757) AGABI2DRAFT192993(AGABI2DRAFT_192993)
 » show all
Taxonomy
Reference
1  [PMID:22004347]
  Authors
Phillips CM, Beeson WT, Cate JH, Marletta MA
  Title
Cellobiose dehydrogenase and a copper-dependent polysaccharide monooxygenase potentiate cellulose degradation by Neurospora crassa.
  Journal
ACS Chem Biol 6:1399-406 (2011)
DOI:10.1021/cb200351y
  Sequence
[ncr:NCU08760]
Reference
2  [PMID:22188218]
  Authors
Beeson WT, Phillips CM, Cate JH, Marletta MA
  Title
Oxidative cleavage of cellulose by fungal copper-dependent polysaccharide monooxygenases.
  Journal
J Am Chem Soc 134:890-2 (2012)
DOI:10.1021/ja210657t
  Sequence
[ncr:NCU08760]
Reference
3  [PMID:22578542]
  Authors
Li X, Beeson WT 4th, Phillips CM, Marletta MA, Cate JH
  Title
Structural basis for substrate targeting and catalysis by fungal polysaccharide monooxygenases.
  Journal
Structure 20:1051-61 (2012)
DOI:10.1016/j.str.2012.04.002
Reference
4  [PMID:23124232]
  Authors
Bey M, Zhou S, Poidevin L, Henrissat B, Coutinho PM, Berrin JG, Sigoillot JC
  Title
Cello-oligosaccharide oxidation reveals differences between two lytic polysaccharide monooxygenases (family GH61) from Podospora anserina.
  Journal
Appl Environ Microbiol 79:488-96 (2013)
DOI:10.1128/AEM.02942-12
  Sequence
Reference
5  [PMID:26185526]
  Authors
Frommhagen M, Sforza S, Westphal AH, Visser J, Hinz SW, Koetsier MJ, van Berkel WJ, Gruppen H, Kabel MA
  Title
Discovery of the combined oxidative cleavage of plant xylan and cellulose by a new fungal polysaccharide monooxygenase.
  Journal
Biotechnol Biofuels 8:101 (2015)
DOI:10.1186/s13068-015-0284-1
Reference
6  [PMID:27213015]
  Authors
Patel I, Kracher D, Ma S, Garajova S, Haon M, Faulds CB, Berrin JG, Ludwig R, Record E
  Title
Salt-responsive lytic polysaccharide monooxygenases from the mangrove fungus Pestalotiopsis sp. NCi6.
  Journal
Biotechnol Biofuels 9:108 (2016)
DOI:10.1186/s13068-016-0520-3
Reference
7  [PMID:27152023]
  Authors
Courtade G, Wimmer R, Rohr AK, Preims M, Felice AK, Dimarogona M, Vaaje-Kolstad G, Sorlie M, Sandgren M, Ludwig R, Eijsink VG, Aachmann FL
  Title
Interactions of a fungal lytic polysaccharide monooxygenase with beta-glucan substrates and cellobiose dehydrogenase.
  Journal
Proc Natl Acad Sci U S A 113:5922-7 (2016)
DOI:10.1073/pnas.1602566113
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.54
IUBMB Enzyme Nomenclature: 1.14.99.54
ExPASy - ENZYME nomenclature database: 1.14.99.54
BRENDA, the Enzyme Database: 1.14.99.54

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