KEGG   ENZYME: 1.14.99.55Help
Entry
EC 1.14.99.55               Enzyme                                 

Name
lytic starch monooxygenase;
LPMO (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
BRITE hierarchy
Sysname
starch, hydrogen-donor:oxygen oxidoreductase (D-glucosyl C1-hydroxylating)
Reaction(IUBMB)
starch + reduced acceptor + O2 = D-glucono-1,5-lactone-terminated malto-oligosaccharides + short-chain malto-oligosaccharides + acceptor + H2O
Substrate
starch [CPD:C00369];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]
Product
D-glucono-1,5-lactone-terminated malto-oligosaccharides;
short-chain malto-oligosaccharides;
acceptor [CPD:C00028];
H2O [CPD:C00001]
Comment
The enzyme cleaves starch in an oxidative manner. It releases fragments of starch with a D-glucono-1,5-lactone at the reducing end. The initially formed alpha-D-glucono-1,5-lactone at the reducing end of the shortend amylose chain quickly hydrolyses spontaneously to the aldonic acid. In vitro ascorbate has been found to be able to serve as reducing agent. The enzyme contains copper at the active site.
History
EC 1.14.99.55 created 2017
Orthology
K00516  lytic starch monooxygenase
Genes
NCR: NCU08746
NTE: NEUTE1DRAFT130221(NEUTE1DRAFT_130221)
SMP: SMAC_06790
PAN: PODANSg579
MTM: MYCTH_2313229
MGR: MGG_10208
FGR: FGSG_04704
FVR: FVEG_07686
FOX: FOXG_04534 FOXG_18882
NHE: NECHADRAFT_83307
PTE: PTT_14495
 » show all
Taxonomy
Reference
1  [PMID:25201969]
  Authors
Vu VV, Beeson WT, Span EA, Farquhar ER, Marletta MA
  Title
A family of starch-active polysaccharide monooxygenases.
  Journal
Proc Natl Acad Sci U S A 111:13822-7 (2014)
DOI:10.1073/pnas.1408090111
  Sequence
[ncr:NCU08746]
Reference
2  [PMID:24828494]
  Authors
Gudmundsson M, Kim S, Wu M, Ishida T, Momeni MH, Vaaje-Kolstad G, Lundberg D, Royant A, Stahlberg J, Eijsink VG, Beckham GT, Sandgren M
  Title
Structural and electronic snapshots during the transition from a Cu(II) to Cu(I)  metal center of a lytic polysaccharide monooxygenase by X-ray photoreduction.
  Journal
J Biol Chem 289:18782-92 (2014)
DOI:10.1074/jbc.M114.563494
Reference
3  [PMID:25608804]
  Authors
Lo Leggio L, Simmons TJ, Poulsen JC, Frandsen KE, Hemsworth GR, Stringer MA, von Freiesleben P, Tovborg M, Johansen KS, De Maria L, Harris PV, Soong CL, Dupree P, Tryfona T, Lenfant N, Henrissat B, Davies GJ, Walton PH
  Title
Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase.
  Journal
Nat Commun 6:5961 (2015)
DOI:10.1038/ncomms6961
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.55
IUBMB Enzyme Nomenclature: 1.14.99.55
ExPASy - ENZYME nomenclature database: 1.14.99.55
BRENDA, the Enzyme Database: 1.14.99.55

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