KEGG   ENZYME: 1.14.99.58
Entry
EC 1.14.99.58               Enzyme                                 

Name
heme oxygenase (biliverdin-IX-beta and delta-forming);
pigA (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
Sysname
protoheme,donor:oxygen oxidoreductase (biliverdin-IX-beta and delta-forming)
Reaction(IUBMB)
(1) protoheme + 3 reduced acceptor + 3 O2 = biliverdin-IX-delta + CO + Fe2+ + 3 acceptor + 3 H2O [RN:R11816];
(2) protoheme + 3 reduced acceptor + 3 O2 = biliverdin-IX-beta + CO + Fe2+ + 3 acceptor + 3 H2O [RN:R11817]
Reaction(KEGG)
R11816 R11817
Substrate
protoheme [CPD:C00032];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]
Product
biliverdin-IX-delta [CPD:C21723];
CO [CPD:C00237];
Fe2+ [CPD:C14818];
acceptor [CPD:C00028];
H2O [CPD:C00001];
biliverdin-IX-beta [CPD:C21724]
Comment
The enzyme, characterized from the bacterium Pseudomonas aeruginosa, differs from EC 1.14.15.20, heme oxygenase (biliverdin-producing, ferredoxin), in that the heme substrate is rotated by approximately 110 degrees within the active site, resulting in cleavage at a different part of the ring. It forms a mixture of about 70% biliverdin-IX-delta and 30% biliverdin-IX-beta.
History
EC 1.14.99.58 created 2017
Pathway
ec00860  Porphyrin and chlorophyll metabolism
Orthology
K07215  heme oxygenase (biliverdin-IX-beta and delta-forming)
Genes
XSA: SB85_17620
XHY: FZ025_13240
SML: Smlt3896(pigA)
SMT: Smal_3312
BUJ: BurJV3_3345
SMZ: SMD_3498(hemO)
SRH: BAY15_3051
SLM: BIZ42_06025
STEN: CCR98_17210
STEM: CLM74_17440
PAE: PA0672(hemO)
PAEV: N297_691
PAEI: N296_691
PAU: PA14_55580(nemO)
PAG: PLES_46571(hemO)
PAF: PAM18_4366(hemO)
PNC: NCGM2_1335(nemO)
PAEB: NCGM1900_2097(nemO)
PAEP: PA1S_23245
PAEM: U769_23030
PAEL: T223_23770
PAEG: AI22_10820
PAEC: M802_689
PAEO: M801_691
PMY: Pmen_4507
PPU: PP_1005(hemO) PP_2582
PPT: PPS_1032
PPUH: B479_05305
PPUN: PP4_32840 PP4_43110(hemO)
PMON: X969_03390
PMOT: X970_03365
PSB: Psyr_1104
PSYR: N018_20130
PFL: PFL_4628(hemO)
PPRO: PPC_4730(hemO)
PFO: Pfl01_4380(hemO)
PFS: PFLU_4969
PFE: PSF113_1273(hemO)
PFC: PflA506_4278(hemO)
PFB: VO64_1844
PFW: PF1751_v1c44610(hemO)
PEN: PSEEN4423
PKC: PKB_3153
PSES: PSCI_0686
PSEM: TO66_24120
PSOS: POS17_4695(hemO)
PANR: A7J50_4738
PSET: THL1_4048
PSIL: PMA3_23130
POJ: PtoMrB4_00820(hemO)
ABM: ABSDF2281(hemO)
ABN: AB57_0991
ABH: M3Q_1216
ABAZ: P795_13120
ABAU: IX87_00955
ACI: ACIAD1478(hemO)
AGU: AS4_16540(hemO) AS4_29790(hemO) AS4_34070(hemO)
AUG: URS_0079
MCT: MCR_0810
MCS: DR90_1063(hemO)
PSPO: PSPO_b1580(hemO)
CJA: CJA_2682
MEJ: Q7A_2860
AXE: P40_13405
NME: NMB1669
NMP: NMBB_1917
NMA: NMA1927
NMW: NMAA_1385(hemO)
NMX: NMA510612_2169(hemO)
NMC: NMC1587(hemO)
NMN: NMCC_1580(hemO)
NMT: NMV_0703(hemO)
NMI: NMO_1487(hemO)
NGO: NGO1318
NGK: NGK_1541
NLA: NLA_6120(hemO)
CHAE: CH06BL_03200(hemO)
AMAH: DLM_2969
CTES: O987_09790
HSE: Hsero_4544(hemO)
HRB: Hrubri_4500(hemO)
ATU: Atu2502(hemO)
AGR: AGROH133_08500(hemO)
ATF: Ach5_23710(hemO)
RIR: BN877_I2583(hemO)
NEN: NCHU2750_34870(hemO)
KAI: K32_07540(hemO)
RPA: RPA3279(pigA)
RPT: Rpal_3698
XAU: Xaut_2445
AZC: AZC_2040
HDN: Hden_2796
BVR: BVIR_2196
BLAG: BLTE_19160(hemO)
CID: P73_1096
STAX: MC45_16995
SPHI: TS85_04935
SECH: B18_15010
GOH: B932_3435
GXL: H845_716
APK: APA386B_225(pigA)
ASZ: ASN_1866(pigA)
TXI: TH3_20460
FTJ: FTUN_5388
 » show all
Reference
1  [PMID:11591684]
  Authors
Ratliff M, Zhu W, Deshmukh R, Wilks A, Stojiljkovic I
  Title
Homologues of neisserial heme oxygenase in gram-negative bacteria: degradation of heme by the product of the pigA gene of Pseudomonas aeruginosa.
  Journal
J Bacteriol 183:6394-403 (2001)
DOI:10.1128/JB.183.21.6394-6403.2001
Reference
2  [PMID:12475329]
  Authors
Caignan GA, Deshmukh R, Wilks A, Zeng Y, Huang HW, Moenne-Loccoz P, Bunce RA, Eastman MA, Rivera M
  Title
Oxidation of heme to beta- and delta-biliverdin by Pseudomonas aeruginosa heme oxygenase as a consequence of an unusual seating of the heme.
  Journal
J Am Chem Soc 124:14879-92 (2002)
DOI:10.1021/ja0274960
Reference
3  [PMID:15122889]
  Authors
Friedman J, Lad L, Li H, Wilks A, Poulos TL
  Title
Structural basis for novel delta-regioselective heme oxygenation in the opportunistic pathogen Pseudomonas aeruginosa.
  Journal
Biochemistry 43:5239-45 (2004)
DOI:10.1021/bi049687g
  Sequence
[pae:PA0672]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.58
IUBMB Enzyme Nomenclature: 1.14.99.58
ExPASy - ENZYME nomenclature database: 1.14.99.58
BRENDA, the Enzyme Database: 1.14.99.58

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