KEGG   ENZYME: 1.14.99.66Help
Entry
EC 1.14.99.66               Enzyme                                 

Name
[histone H3]-N6,N6-dimethyl-L-lysine4 FAD-dependent demethylase;
KDM1 (gene name);
LSD1 (gene name);
lysine-specific histone demethylase 1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
BRITE hierarchy
Sysname
[histone H3]-N6,N6-dimethyl-L-lysine4:acceptor oxidoreductase (demethylating)
Reaction(IUBMB)
a [histone H3]-N6,N6-dimethyl-L-lysine4 + 2 acceptor + 2 H2O = a [histone H3]-L-lysine4 + 2 formaldehyde + 2 reduced acceptor (overall reaction) [RN:R12462];
(1a) a [histone H3]-N6,N6-dimethyl-L-lysine4 + acceptor + H2O = a [histone H3]-N6-methyl-L-lysine4 + formaldehyde + reduced acceptor [RN:R12463];
(1b) a [histone H3]-N6-methyl-L-lysine4 + acceptor + H2O = a [histone H3]-L-lysine4 + formaldehyde + reduced acceptor [RN:R12464]
Reaction(KEGG)
Substrate
[histone H3]-N6,N6-dimethyl-L-lysine4;
acceptor [CPD:C00028];
H2O [CPD:C00001];
[histone H3]-N6-methyl-L-lysine4
Product
[histone H3]-L-lysine4;
formaldehyde [CPD:C00067];
reduced acceptor [CPD:C00030];
[histone H3]-N6-methyl-L-lysine4
Comment
The enzyme specifically removes methyl groups from mono- and dimethylated lysine4 of histone 3. During the reaction the substrate is oxidized by the FAD cofactor of the enzyme to generate the corresponding imine, which is subsequently hydrolysed in the form of formaldehyde.The enzyme is similar to flavin amine oxidases, and differs from all other known histone lysine demethylases, which are iron(II)- and 2-oxoglutarate-dependent dioxygenases. The physiological electron acceptor is not known with certainty. In vitro the enzyme can use oxygen, which is reduced to hydrogen peroxide, but generation of hydrogen peroxide in the chromatin environment is unlikely as it will result in oxidative damage of DNA.
History
EC 1.14.99.66 created 2019
Orthology
K11450  [histone H3]-N6,N6-dimethyl-L-lysine4 FAD-dependent demethylase
Genes
HSA: 23028(KDM1A)
PTR: 456614(KDM1A)
PPS: 100995039(KDM1A)
GGO: 101144258(KDM1A)
PON: 100171870(KDM1A)
NLE: 100579631(KDM1A)
MCC: 718609(KDM1A)
MCF: 102135743(KDM1A)
CSAB: 103225404(KDM1A)
RRO: 104663232(KDM1A)
RBB: 108538436(KDM1A)
CJC: 100406478(KDM1A)
SBQ: 101034653(KDM1A)
MMU: 99982(Kdm1a)
MCAL: 110292704(Kdm1a)
MPAH: 110322979(Kdm1a)
RNO: 500569(Kdm1a)
MUN: 110545399(Kdm1a)
CGE: 100770721(Kdm1a)
NGI: 103746403(Kdm1a)
HGL: 101706376(Kdm1a)
CCAN: 109681855(Kdm1a)
OCU: 100358443(KDM1A)
TUP: 102491246(KDM1A)
CFA: 478193(KDM1A)
VVP: 112930162(KDM1A)
AML: 100469590(KDM1A)
UMR: 103666569(KDM1A)
UAH: 113268534(KDM1A)
ORO: 101371349(KDM1A)
ELK: 111154639
FCA: 101096583(KDM1A)
PTG: 102952813(KDM1A)
PPAD: 109274289(KDM1A)
AJU: 106977650(KDM1A)
BTA: 532997(KDM1A)
BOM: 102287646(KDM1A)
BIU: 109574017(KDM1A)
BBUB: 102396562(KDM1A)
CHX: 102185879(KDM1A)
OAS: 101121409(KDM1A)
SSC: 100127157(KDM1A)
CFR: 102517288(KDM1A)
CDK: 105088880(KDM1A)
BACU: 103012479(KDM1A)
LVE: 103084888(KDM1A)
OOR: 101273117(KDM1A)
DLE: 111163462(KDM1A)
PCAD: 102995575(KDM1A)
ECB: 100071646(KDM1A)
EPZ: 103551290(KDM1A)
EAI: 106839994(KDM1A)
MYB: 102261213(KDM1A)
MYD: 102754604(KDM1A)
MNA: 107538326(KDM1A)
HAI: 109375888(KDM1A)
DRO: 112299203(KDM1A)
PALE: 102887129(KDM1A)
RAY: 107513066(KDM1A)
MJV: 108391096(KDM1A)
LAV: 100663912(KDM1A)
TMU: 101347919
MDO: 100025161(KDM1A)
PCW: 110206041(KDM1A)
OAA: 100086658(KDM1A)
GGA: 419571(KDM1A)
MGP: 100544521(KDM1A)
CJO: 107323904(KDM1A)
NMEL: 110387355(KDM1A)
APLA: 101803659(KDM1A)
ACYG: 106046571(KDM1A)
TGU: 100221744(KDM1A)
LSR: 110484269(KDM1A)
SCAN: 103822578(KDM1A)
GFR: 102041827(KDM1A)
FAB: 101810697(KDM1A)
PHI: 102109140(KDM1A)
PMAJ: 107214056(KDM1A)
CCAE: 111939020(KDM1A)
CCW: 104697964(KDM1A)
ETL: 114061578(KDM1A)
FPG: 101923221(KDM1A)
FCH: 102058829(KDM1A)
CLV: 102088529(KDM1A) 102094209
EGZ: 104132548(KDM1A)
NNI: 104015516(KDM1A)
ACUN: 113488263(KDM1A)
PADL: 103925455(KDM1A)
AAM: 106484726(KDM1A)
ASN: 102375236(KDM1A)
AMJ: 102563192(KDM1A)
PSS: 102460305(KDM1A)
CMY: 102931629(KDM1A)
CPIC: 101940420(KDM1A)
ACS: 100557968(kdm1a)
PVT: 110089669(KDM1A)
PBI: 103067389(KDM1A)
PMUR: 107288027(KDM1A)
TSR: 106540788(KDM1A)
PMUA: 114603591(KDM1A)
GJA: 107116662(KDM1A)
XLA: 100127344(kdm1a.S) 108708259(kdm1a.L)
XTR: 100216250(kdm1a)
NPR: 108788279(KDM1A)
DRE: 558450(kdm1a)
CCAR: 109048821(kdm1a) 109105304
IPU: 108269802(kdm1a)
PHYP: 113533951(kdm1a)
AMEX: 103023119(kdm1a)
EEE: 113584671(kdm1a)
TRU: 101073663(kdm1a)
LCO: 104931691(kdm1a)
NCC: 104968042(kdm1a)
MZE: 101466759(kdm1a)
ONL: 100702505(kdm1a)
OLA: 101172293(kdm1a)
XMA: 102231844(kdm1a)
XCO: 114134181(kdm1a)
PRET: 103459946(kdm1a)
CVG: 107084713(kdm1a)
NFU: 107392201(kdm1a)
KMR: 108236889(kdm1a)
ALIM: 106526345(kdm1a) 106533898
AOCE: 111573331(kdm1a)
CSEM: 103378492(kdm1a)
POV: 109636394(kdm1a)
LCF: 108874888
SDU: 111226160(kdm1a)
SLAL: 111655910(kdm1a)
HCQ: 109509193 109526830(kdm1a)
BPEC: 110161360(kdm1a)
MALB: 109955009(kdm1a)
ELS: 105016064(kdm1a)
SFM: 108922806(kdm1a)
PKI: 111854724(kdm1a)
LCM: 102363978(KDM1A)
CMK: 103179609(kdm1a)
RTP: 109926736(kdm1a)
CIN: 100187100
SPU: 575875
APLC: 110990427
SKO: 100373836
DME: Dmel_CG17149(Su(var)3-3)
DER: 6545751
DSI: Dsimw501_GD14861(Dsim_GD14861)
DSR: 110178555
DPE: 6596031
DMN: 108152784
DWI: 6638853
DAZ: 108613120
DNV: 108651092
DHE: 111600951
MDE: 101897898
LCQ: 111681191
AAG: 5574767
AALB: 109422398
AME: 726469
BIM: 100740140
BTER: 100647583
CCAL: 108622958
OBB: 114871968
SOC: 105200954
MPHA: 105832792
AEC: 105143630
PBAR: 105433548
VEM: 105564973
HST: 105185849
DQU: 106751689
CFO: 105253997
LHU: 105674077
PGC: 109854993
OBO: 105282940
NVI: 100678665
MDL: 103580293
TCA: 103315031
DPA: 109547044
ATD: 109597000
BMOR: 101739058
PRAP: 110998007
HAW: 110370308
PXY: 105386718(bursa)
API: 100168654
RMD: 113549832
FCD: 110853963
PVM: 113807228
TUT: 107368558
CSCU: 111638896
PTEP: 107446922
CEL: CELE_T08D10.2(lsd-1) CELE_Y40B1B.6(spr-5)
CBR: CBG08015(Cbr-spr-5)
BMY: Bm1_20500
PCAN: 112561001
CRG: 105346267
MYI: 110454266
OBI: 106869849
LAK: 106170923
EPA: 110237306
ADF: 107335615
AMIL: 114968998
PDAM: 113675980
SPIS: 111342045
DGT: 114533681
HMG: 100202389
AQU: 100631436
ATH: AT1G62830(LDL1) AT3G10390(FLD) AT3G13682(LDL2)
LJA: Lj0g3v0244869.1(Lj0g3v0244869.1) Lj2g3v0743120.1(Lj2g3v0743120.1) Lj3g3v1249990.1(Lj3g3v1249990.1) Lj3g3v1249990.2(Lj3g3v1249990.2) Lj3g3v1249990.3(Lj3g3v1249990.3)
DOSA: Os02t0755200-01(Os02g0755200) Os04t0560300-01(Os04g0560300) Os08t0143400-01(Os08g0143400)
ATS: 109741795(LOC109741795) 109757419(LOC109757419) 109781192(LOC109781192)
OLU: OSTLU_42289(AOX1)
NCR: NCU09120
NTE: NEUTE1DRAFT90788(NEUTE1DRAFT_90788)
MGR: MGG_09915
SSCK: SPSK_08325
NHE: NECHADRAFT_37590(HDMA2101)
MAJ: MAA_05739
CMT: CCM_02339
MBE: MBM_02431
ANI: AN3580.2
ANG: ANI_1_2824014(An01g08580)
ABE: ARB_00124
TVE: TRV_06665
PTE: PTT_10530
DFA: DFA_01745
 » show all
Taxonomy
Reference
1  [PMID:15811342]
  Authors
Forneris F, Binda C, Vanoni MA, Mattevi A, Battaglioli E
  Title
Histone demethylation catalysed by LSD1 is a flavin-dependent oxidative process.
  Journal
FEBS Lett 579:2203-7 (2005)
  Sequence
[hsa:23028]
Reference
2  [PMID:19624733]
  Authors
Forneris F, Battaglioli E, Mattevi A, Binda C
  Title
New roles of flavoproteins in molecular cell biology: histone demethylase LSD1 and chromatin.
  Journal
FEBS J 276:4304-12 (2009)
  Sequence
[hsa:23028]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.66
IUBMB Enzyme Nomenclature: 1.14.99.66
ExPASy - ENZYME nomenclature database: 1.14.99.66
BRENDA, the Enzyme Database: 1.14.99.66

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