KEGG ENZYME: 1.17.1.3

ENZYME: 1.17.1.3

Entry

EC 1.17.1.3                 Enzyme

Name

leucoanthocyanidin reductase;
leucocyanidin reductase

Class

Oxidoreductases;
Acting on CH or CH2 groups;
With NAD+ or NADP+ as acceptor

Sysname

(2R,3S)-catechin:NADP+ 4-oxidoreductase

Reaction(IUBMB)

(2R,3S)-catechin + NADP+ + H2O = 2,3-trans-3,4-cis-leucocyanidin + NADPH + H+ [RN:R06532]

Reaction(KEGG)

R06532;
(other) R06533 R06615

Substrate

(2R,3S)-catechin [CPD:C06562];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Product

2,3-trans-3,4-cis-leucocyanidin [CPD:C05906];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

The enzyme catalyses the synthesis of catechin, catechin-4beta-ol (leucocyanidin) and the related flavan-3-ols afzelechin and gallocatechin, which are initiating monomers in the synthesis of plant polymeric proanthocyanidins or condensed tannins. While 2,3-trans-3,4-cis-leucocyanidin is the preferred flavan-3,4-diol substrate, 2,3-trans-3,4-cis-leucodelphinidin and 2,3-trans-3,4-cis-leucopelargonidin can also act as substrates, but more slowly. NADH can replace NADPH but is oxidized more slowly.

History

EC 1.17.1.3 created 2003

Pathway

ec00941

Flavonoid biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K13081

leucoanthocyanidin reductase

Genes

CPAP:

110824010

CIT:

102621411

CIC:	CICLE_v10033821mg

PVY:

116124900

MINC:

123213776 123215765

TCC:	18604056(LAR) 18610545

GRA:	105764571 105765625

GHI:	107931042 107934794 107946451 121224433

GAB:	108454436 108476741

HSYR:

120118425 120193329

DZI:	111290788 111316766 111318411

EGR:	104426102 104426103 104433307

RARG:

115734981 115755536

GMX:	100786879(LAR1) 100795100

GSJ:	114370702 114402606

PVU:

137829660

VRA:

106771362

VAR:

108324973

VUN:

114190745

VUM:	124829599 124829602

CCAJ:

109813851

APRC:

113869601 113873201

MTR:

11406967

TPRA:

123920205

CAM:

101493305

PSAT:

127097507

VVO:

131613796

LJA:	130720226 130739595

ADU:

107491282

AIP:

107643784

AHF:	112750525 112802690

LANG:

109356072

PCIN:

129299239 129311251 129321631

QSA:	O6P43_002303 O6P43_023007

FVE:

101306809

AANS:

126790297

RCN:

112199978

PPER:

18789589

PMUM:

103341429

PAVI:

110744276 110754077

PDUL:

117616123 117627654

MDM:	103402727(LAR1) 103420366 103451680(LAR2)

MSYL:

126594846 126607464 126626083

PXB:	103929197 103938522 103947071

PCOX:

137711618 137731556 137737443

ZJU:	107418854 107429483

MNT:	112091008 21405124

RCU:

8269513

MEAN:

126674438

JCU:

105641976

HBR:	110649288 110666021

MESC:

110617547 110630746

POP:	7466920 7475620 7483928

PEU:	105113933 105123652

PALZ:

118037319 118039969 118047583

PNZ:	133675876 133697221 133704982

JRE:	108993261 108994581 109000235 109002691

CILL:

122295621 122299102 122299405

CAVE:

132184662 132184746

AGLU:

133865496 133866636

QSU:	112024706 112036510

QLO:	115963778 115965626 115991055

TWL:	120001998 120008990

VVI:	100232982(LAR1) 100233112(LAR2)

VRI:

117911022

SPEN:

114077302

SOT:

102594738

SSTN:

125868902

LBB:

132641829

NTA:	107793724 107815501

NSY:

104222673

NTO:

104107265

NAU:

109235276

OEU:

111369334

HAN:

110917901

CSIN:

114263727 114266906 114323746

RVL:	131300846 131310145 131310636

AEW:	130755474 130761006 130765196 130772573 130790641 130792909

DLT:

127797457

BVG:

104908227

SOE:

110789492

CQI:	110697307 110726068

ATRI:

130798457

MOF:	131146767 131153426

NNU:	104601230 104603047

MING:

122080354 122084111

TSS:	122641046 122663687

PSOM:

113273916 113277573

OSA:

4332311

DOSA:

Os03g0259400

OBR:

102717501

OGL:

127767860

BDI:

100839668

ATS:

109751108

TDC:	119288727 119293758 119320953

TAES:

123081808 123094982 123098003 123175387

TUA:	125553985 125553987

LPER:

127349037

LRD:	124699354 124699938

PAUA:

133910968

PDA:	103696132 103700891

EGU:	105041053 105048528

MUS:

103974395

AOF:

109834931

MSIN:

131226926 131251422

ACAK:

137956707

NCOL:

116259067 116262645 116262876 116262877 116262878

ATR:

18423736

CJF:	131027773 131029258 131029267 131029272 131029280 131029282 131029283 131029286 131029312 131029318 131070694 131860846 131860847 131860848 131860849 131860850 131860851 131860852 131861616 131861617

» show all

Reference

1 [PMID:8470799]

Authors

Tanner GJ, Kristiansen KN.

Title

Synthesis of 3,4-cis-[3H]leucocyanidin and enzymatic reduction to catechin.

Journal

Anal Biochem 209:274-7 (1993)
DOI:10.1006/abio.1993.1119

Reference

2 [PMID:12788945]

Authors

Tanner GJ, Francki KT, Abrahams S, Watson JM, Larkin PJ, Ashton AR

Title

Proanthocyanidin biosynthesis in plants. Purification of legume leucoanthocyanidin reductase and molecular cloning of its cDNA.

Journal

J Biol Chem 278:31647-56 (2003)
DOI:10.1074/jbc.M302783200

Sequence

[ag:CAD79341]

Other DBs

ExplorEnz - The Enzyme Database:

1.17.1.3

IUBMB Enzyme Nomenclature:

1.17.1.3

ExPASy - ENZYME nomenclature database:

1.17.1.3

BRENDA, the Enzyme Database:

1.17.1.3

CAS:	776323-46-7

All links

Gene (4)   
   MGENES (3)   
   RefGene (1)   
Protein sequence (61)   
   UniProt (45)   
   SWISS-PROT (1)   
   RefSeq(pep) (11)   
   PDBSTR (4)   
DNA sequence (64)   
   RefSeq(nuc) (11)   
   GenBank (22)   
   EMBL (31)   
3D Structure (4)   
   PDB (4)   
Protein domain (4)   
   InterPro (4)   
All databases (137)   

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