KEGG ENZYME: 1.17.1.3

ENZYME: 1.17.1.3

Entry

EC 1.17.1.3 Enzyme

Name

leucoanthocyanidin reductase;
leucocyanidin reductase

Class

Oxidoreductases;
Acting on CH or CH2 groups;
With NAD+ or NADP+ as acceptor

Sysname

(2R,3S)-catechin:NADP+ 4-oxidoreductase

Reaction(IUBMB)

(2R,3S)-catechin + NADP+ + H2O = 2,3-trans-3,4-cis-leucocyanidin + NADPH + H+ [RN:R06532]

Reaction(KEGG)

R06532;
(other) R06533 R06615

Substrate

(2R,3S)-catechin [CPD:C06562];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Product

2,3-trans-3,4-cis-leucocyanidin [CPD:C05906];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

The enzyme catalyses the synthesis of catechin, catechin-4beta-ol (leucocyanidin) and the related flavan-3-ols afzelechin and gallocatechin, which are initiating monomers in the synthesis of plant polymeric proanthocyanidins or condensed tannins. While 2,3-trans-3,4-cis-leucocyanidin is the preferred flavan-3,4-diol substrate, 2,3-trans-3,4-cis-leucodelphinidin and 2,3-trans-3,4-cis-leucopelargonidin can also act as substrates, but more slowly. NADH can replace NADPH but is oxidized more slowly.

History

EC 1.17.1.3 created 2003

Pathway

ec00941

Flavonoid biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K13081

leucoanthocyanidin reductase

Genes

CPAP:

110824010

CIT:

102621411

CIC:	CICLE_v10033821mg

TCC:	18604056(LAR) 18610545

GRA:	105764571(lar1) 105765625(lar2)

GHI:	107931042 107934794 107946451

GAB:	108454436 108476741

DZI:	111290788 111316766 111318411

EGR:	104426102 104426103 104433307

GMX:	100786879(LAR1) 100795100

GSJ:	114370702 114402606

PVU:	PHAVU_007G102100g

VRA:

VAR:

VUN:

CCAJ:

MTR:	MTR_1g093720

CAM:

101493305

LJA:	Lj2g3v1172450.1(Lj2g3v1172450.1)

ADU:

AIP:

LANG:

FVE:

RCN:

PPER:

PMUM:

PAVI:

MDM:	103402727(LAR1) 103451680(LAR2) 108169764

PXB:	103929197 103938522 103947071

ZJU:	107418854 107429483

RCU:

8269513

JCU:

105641976

HBR:	110649288 110666021

MESC:

110617547 110630746

POP:	7466920 7475620 7483928

PEU:	105113933 105123652

JRE:	108993261 108994581 109000235 109002691

QSU:	112024706 112036510

VVI:	100232982(LAR1) 100233112(LAR2)

SPEN:

114077302

SOT:

102594738

NTA:	107793724 107815501

NSY:

NTO:

NAU:

OEU:

HAN:

BVG:

SOE:

CQI:	110697307 110726068

NNU:	104601230 104603047

PSOM:

113273916 113277573

OSA:

4332311

DOSA:

Os03t0259400-00(Os03g0259400)

OBR:

102717501

BDI:

100839668

ATS:	109751108(LOC109751108)

PDA:	103696132 103700891

EGU:	105041053 105048528

MUS:

AOF:

ATR:

Reference

1 [PMID:8470799]

Authors

Tanner GJ, Kristiansen KN.

Title

Synthesis of 3,4-cis-[3H]leucocyanidin and enzymatic reduction to catechin.

Journal

Anal Biochem 209:274-7 (1993)
DOI:10.1006/abio.1993.1119

Reference

2 [PMID:12788945]

Authors

Tanner GJ, Francki KT, Abrahams S, Watson JM, Larkin PJ, Ashton AR.

Title

Proanthocyanidin biosynthesis in plants. Purification of legume leucoanthocyanidin reductase and molecular cloning of its cDNA.

Journal

J Biol Chem 278:31647-56 (2003)
DOI:10.1074/jbc.M302783200

Sequence

[ag:CAD79341]

Other DBs

ExplorEnz - The Enzyme Database:

1.17.1.3

IUBMB Enzyme Nomenclature:

1.17.1.3

ExPASy - ENZYME nomenclature database:

1.17.1.3

BRENDA, the Enzyme Database:

1.17.1.3

CAS:	776323-46-7

DBGET integrated database retrieval system