KEGG ENZYME: 1.17.1.5

ENZYME: 1.17.1.5

Entry

EC 1.17.1.5                 Enzyme

Name

nicotinate dehydrogenase;
nicotinic acid hydroxylase;
nicotinate hydroxylase

Class

Oxidoreductases;
Acting on CH or CH2 groups;
With NAD+ or NADP+ as acceptor

Sysname

nicotinate:NADP+ 6-oxidoreductase (hydroxylating)

Reaction(IUBMB)

nicotinate + H2O + NADP+ = 6-hydroxynicotinate + NADPH + H+ [RN:R01720]

Reaction(KEGG)

R01720

Substrate

nicotinate [CPD:C00253];
H2O [CPD:C00001];
NADP+ [CPD:C00006]

Product

6-hydroxynicotinate [CPD:C01020];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

A flavoprotein containing non-heme iron. The enzyme is capable of acting on a variety of nicotinate analogues to varying degrees, including pyrazine-2-carboxylate, pyrazine 2,3-dicarboxylate, trigonelline and 6-methylnicotinate. The enzyme from Clostridium barkeri also possesses a catalytically essential, labile selenium that can be removed by reaction with cyanide.

History

EC 1.17.1.5 created 1972 as EC 1.5.1.13, transferred 2004 to EC 1.17.1.5

Pathway

ec00760

Nicotinate and nicotinamide metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K20445

nicotinate dehydrogenase FAD-subunit

K20446

nicotinate dehydrogenase small FeS subunit

K20447

nicotinate dehydrogenase large molybdopterin subunit

K20448

nicotinate dehydrogenase medium molybdopterin subunit

Genes

SOF:	NCTC11214_04501(ndhM)

HAXI:

HAALTHF_35790n

UPI:	EJG51_001725

BVZ:	BRAD3257_6051

MALU:

KU6B_09420 KU6B_24970

VSU:	P3M64_05615

DTI:	Desti_0416 Desti_0691

CPSS:

M5V91_09065

CPAT:

CLPA_c02860(ndhF1) CLPA_c02870(ndhS1) CLPA_c02880(ndhL)

CPAE:

CPAST_c02860(ndhF1) CPAST_c02870(ndhS1) CPAST_c02880(ndhL)

CINT:

HZF06_02100

CALD:

PWK10_12180 PWK10_12185

VFA:	MM35RIKEN_03290

ACOL:

K5I23_11315 K5I23_11320 K5I23_11325

PETR:

QKW49_07170

DOR:	Desor_0445 Desor_0446 Desor_1381 Desor_1382

DAI:	Desaci_2061 Desaci_2062 Desaci_2063

DMI:	Desmer_1309 Desmer_1310

FWA:	DCMF_22605 DCMF_25575

AACX:

DEACI_1213 DEACI_1473 DEACI_1474 DEACI_1596 DEACI_3801 DEACI_3802

ACAE:

HYG86_14455 HYG86_14460

PROM:

QO263_02190 QO263_18065 QO263_18070 QO263_18075 QO263_18080

BLIQ:

INP51_10785

BLAR:

LC508_08175

BCOC:

BLCOC_22520(ndhS_3)

ETM:	CE91St48_21740

AMT:

Amet_4569

AOE:	Clos_0375 Clos_0377

TEB:	T8CH_1929(xdhC)

CHY:	CHY_0690(coxM)

MTA:	Moth_1225 Moth_1226

MTHO:

MOTHE_c12030(ndhM) MOTHE_c12040(ndhL)

MTHZ:

MOTHA_c12900(ndhM) MOTHA_c12910(ndhL)

TEP:	TepRe1_0411 TepRe1_0412 TepRe1_0413

TAE:	TepiRe1_0458 TepiRe1_0459 TepiRe1_0460(ndhM)

NTH:	Nther_2150 Nther_2151 Nther_2152 Nther_2153

HHL:	Halha_1050

HPRF:

HLPR_02890(xdhB)

TIS:	P3962_00500 P3962_08805 P3962_08810 P3962_08820

TIZ:	RBU61_01420 RBU61_01430 RBU61_01435

SSPH:

SPSPH_010430(ndhS)

SOVA:

SOV_13250(ndhL) SOV_13260(ndhM)

SSID:

SPSIL_018490(ndhL) SPSIL_018500(ndhM)

ETT:	AZF37_06400

FBS:	N510_000961(ndhF) N510_000962(ndhS)

XCL:	G4Z02_01140

TBK:	HF295_00970

PFLA:

Pflav_009980 Pflav_036150 Pflav_072710 Pflav_072730

IPO:	Ilyop_0111 Ilyop_0112 Ilyop_0113 Ilyop_0114

TFE:	JYK00_05500

PMO:	Pmob_1740 Pmob_1741

» show all

Reference

1 [PMID:4388026]

Authors

Holcenberg JS, Stadtman ER.

Title

Nicotinic acid metabolism. 3. Purification and properties of a nicotinic acid hydroxylase.

Journal

J Biol Chem 244:1194-203 (1969)

Reference

2 [PMID:8555176]

Authors

Gladyshev VN, Khangulov SV, Stadtman TC

Title

Properties of the selenium- and molybdenum-containing nicotinic acid hydroxylase from Clostridium barkeri.

Journal

Biochemistry 35:212-23 (1996)
DOI:10.1021/bi951793i

Sequence

[ag:ABC88396 ABC88397 ABC88398 ABC88399]

Reference

3 [PMID:8278371]

Authors

Gladyshev VN, Khangulov SV, Stadtman TC.

Title

Nicotinic acid hydroxylase from Clostridium barkeri: electron paramagnetic resonance studies show that selenium is coordinated with molybdenum in the catalytically active selenium-dependent enzyme.

Journal

Proc Natl Acad Sci U S A 91:232-6 (1994)
DOI:10.1073/pnas.91.1.232

Reference

4 [PMID:6838209]

Authors

Dilworth GL.

Title

Occurrence of molybdenum in the nicotinic acid hydroxylase from Clostridium barkeri.

Journal

Arch Biochem Biophys 221:565-9 (1983)
DOI:10.1016/0003-9861(83)90176-5

Reference

5 [PMID:7181513]

Authors

Dilworth GL.

Title

Properties of the selenium-containing moiety of nicotinic acid hydroxylase from Clostridium barkeri.

Journal

Arch Biochem Biophys 219:30-8 (1982)
DOI:10.1016/0003-9861(82)90130-8

Reference

Authors

Nagel M, Andreesen JR.

Title

Purification and characterization of the molybdoenzymes nicotinate dehydrogenase and 6-hydroxynicotinate dehydrogenase from Bacillus niacini.

Journal

Arch Microbiol 154:605-613 (1990)

Other DBs

ExplorEnz - The Enzyme Database:

1.17.1.5

IUBMB Enzyme Nomenclature:

1.17.1.5

ExPASy - ENZYME nomenclature database:

1.17.1.5

BRENDA, the Enzyme Database:

1.17.1.5

CAS:

9059-03-4

DBGET integrated database retrieval system