Oxidoreductases;
Acting on CH or CH2 groups;
With a cytochrome as acceptor

nicotinate:cytochrome 6-oxidoreductase (hydroxylating)

nicotinate + a ferricytochrome + H2O = 6-hydroxynicotinate + a ferrocytochrome + 2 H+ [RN:R09473]

R09473

nicotinate [CPD:C00253];
ferricytochrome [CPD:C00923];
H2O [CPD:C00001]

This two-component enzyme from Pseudomonas belongs to the family of xanthine dehydrogenases, but differs from most other members of this family. While most members contain an FAD cofactor, the large subunit of this enzyme contains three c-type cytochromes, enabling it to interact with the electron transfer chain, probably by delivering the electrons to a cytochrome oxidase. The small subunit contains a typical molybdopterin cytosine dinucleotide(MCD) cofactor and two [2Fe-2S] clusters [1].

EC 1.17.2.1 created 2010

1  [PMID:18678916]

Jimenez JI, Canales A, Jimenez-Barbero J, Ginalski K, Rychlewski L, Garcia JL, Diaz E

Deciphering the genetic determinants for aerobic nicotinic acid degradation: the  nic cluster from Pseudomonas putida KT2440.

Proc Natl Acad Sci U S A 105:11329-34 (2008)
DOI:10.1073/pnas.0802273105

[ppu:PP_3947 PP_3948]

2  [PMID:19118407]

Yang Y, Yuan S, Chen T, Ma P, Shang G, Dai Y

Cloning, heterologous expression, and functional characterization of the nicotinate dehydrogenase gene from Pseudomonas putida KT2440.

Biodegradation 20:541-9 (2009)
DOI:10.1007/s10532-008-9243-x

[ppu:PP_3947 PP_3948]