KEGG   ENZYME: 1.17.2.1
Entry
EC 1.17.2.1                 Enzyme                                 

Name
nicotinate dehydrogenase (cytochrome);
nicotinic acid hydroxylase;
nicotinate hydroxylase
Class
Oxidoreductases;
Acting on CH or CH2 groups;
With a cytochrome as acceptor
Sysname
nicotinate:cytochrome 6-oxidoreductase (hydroxylating)
Reaction(IUBMB)
nicotinate + a ferricytochrome + H2O = 6-hydroxynicotinate + a ferrocytochrome + 2 H+ [RN:R09473]
Reaction(KEGG)
R09473
Substrate
nicotinate [CPD:C00253];
ferricytochrome [CPD:C00923];
H2O [CPD:C00001]
Product
6-hydroxynicotinate [CPD:C01020];
ferrocytochrome [CPD:C00924];
H+ [CPD:C00080]
Comment
This two-component enzyme from Pseudomonas belongs to the family of xanthine dehydrogenases, but differs from most other members of this family. While most members contain an FAD cofactor, the large subunit of this enzyme contains three c-type cytochromes, enabling it to interact with the electron transfer chain, probably by delivering the electrons to a cytochrome oxidase. The small subunit contains a typical molybdopterin cytosine dinucleotide(MCD) cofactor and two [2Fe-2S] clusters [1].
History
EC 1.17.2.1 created 2010
Pathway
ec00760  Nicotinate and nicotinamide metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K18029  nicotinate dehydrogenase subunit A
K18030  nicotinate dehydrogenase subunit B
Genes
IZH: FEM41_12580 FEM41_12585
SMAR: SM39_1773 SM39_1774 SM39_1932 SM39_1933
SMAC: SMDB11_1586 SMDB11_1587 SMDB11_1756 SMDB11_1757
SMW: SMWW4_v1c23280 SMWW4_v1c23290 SMWW4_v1c24880 SMWW4_v1c24890
SPE: Spro_2314 Spro_2315 Spro_2491 Spro_2492
SRR: SerAS9_2271 SerAS9_2272
SRL: SOD_c21440(nicA) SOD_c21450(nicB1)
SRY: M621_11745 M621_11750
SPLY: Q5A_011880(nicA) Q5A_011885(nicB)
SRS: SerAS12_2271 SerAS12_2272
SFJ: SAMEA4384070_2383(iorA_1) SAMEA4384070_2384(adhB_1)
SOF: NCTC11214_04485(adhB_2) NCTC11214_04486(iorA_2)
PPU: PP_3947(nicA) PP_3948(nicB)
AMAL: I607_10990
AMAE: I876_11360
AMAO: I634_11215
AMAD: I636_11245
AMAI: I635_11650
AMAG: I533_10995
REH: H16_A0920(h16_A0920) H16_A0921(h16_A0921)
CNC: CNE_1c08700(adhB1) CNE_1c08710(iorA1) CNE_1c08780(iorB1)
CGD: CR3_3004(coxL) CR3_3011(nicA)
BMJ: BMULJ_03958(coxL)
CABA: SBC2_37440(nicB_1) SBC2_37450(nicA_1) SBC2_37530(nicB_2) SBC2_46120(nicB_3) SBC2_46130(nicA_2)
BPE: BP1953
BPC: BPTD_1925
BPER: BN118_0961
BPEU: Q425_14990
BAV: BAV1527 BAV1533(iorA) BAV1534
AFQ: AFA_14975(nicB2) AFA_14980(nicA) AFA_14985(nicB2)
RFR: Rfer_0864
LIM: L103DPR2_00172(nicB_1) L103DPR2_00173(nicA_1) L103DPR2_00692(nicA_2)
HSE: Hsero_1321 Hsero_1322(iorA) Hsero_1327(coxL)
DAL: Dalk_2371
CCX: COCOR_06361(iorA2) COCOR_06362(iorB2)
ARA: Arad_8291 Arad_8292(xdhA)
NEN: NCHU2750_46220(nicA) NCHU2750_46230(nicB)
BJA: bll3820(bll3820) bll3821(bll3821) blr6047(blr6047)
BVZ: BRAD3257_3073(nicA) BRAD3257_5215(nicA) BRAD3257_5216(nicB)
PLEO: OHA_2_00104(nicB) OHA_2_00105(nicA)
HDI: HDIA_4147(nicB_2) HDIA_4148(nicA_1) HDIA_4442(nicA_2) HDIA_4443(nicB_3)
MMED: Mame_02898(nicA) Mame_02899(nicB_3)
PALW: PSAL_015660(nicA) PSAL_015670(nicB_2)
NAR: Saro_1347
ADO: A6F68_00953(nicA)
KSC: CD178_02880(nicB_2) CD178_02881(nicA)
MAGX: XM1_1925 XM1_1926(pucE)
TTR: Tter_2353
OBG: Verru16b_02805(nicB_3) Verru16b_02806(nicA_2)
PND: Pla175_26890(nicB)
AGV: OJF2_57870(nicB)
ABAC: LuPra_02179(nicB_1) LuPra_02180(nicA_1) LuPra_05705(nicA_2)
DORI: FH5T_11980
 » show all
Reference
1  [PMID:18678916]
  Authors
Jimenez JI, Canales A, Jimenez-Barbero J, Ginalski K, Rychlewski L, Garcia JL, Diaz E
  Title
Deciphering the genetic determinants for aerobic nicotinic acid degradation: the nic cluster from Pseudomonas putida KT2440.
  Journal
Proc Natl Acad Sci U S A 105:11329-34 (2008)
DOI:10.1073/pnas.0802273105
  Sequence
Reference
2  [PMID:19118407]
  Authors
Yang Y, Yuan S, Chen T, Ma P, Shang G, Dai Y
  Title
Cloning, heterologous expression, and functional characterization of the nicotinate dehydrogenase gene from Pseudomonas putida KT2440.
  Journal
Biodegradation 20:541-9 (2009)
DOI:10.1007/s10532-008-9243-x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.17.2.1
IUBMB Enzyme Nomenclature: 1.17.2.1
ExPASy - ENZYME nomenclature database: 1.17.2.1
BRENDA, the Enzyme Database: 1.17.2.1

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