KEGG   ENZYME: 1.17.3.4Help
Entry
EC 1.17.3.4                 Enzyme                                 

Name
juglone 3-hydroxylase;
juglone hydroxylase;
naphthoquinone hydroxylase;
naphthoquinone-hydroxylase
Class
Oxidoreductases;
Acting on CH or CH2 groups;
With oxygen as acceptor
BRITE hierarchy
Sysname
5-hydroxy-1,4-naphthoquinone,water:oxygen oxidoreductase (3-hydroxylating)
Reaction(IUBMB)
2 juglone + O2 = 2 3,5-dihydroxy-1,4-naphthoquinone (overall reaction) [RN:R04327];
(1a) 2 juglone + 2 H2O = 2 naphthalene-1,2,4,8-tetrol [RN:R11495];
(1b) 2 naphthalene-1,2,4,8-tetrol + O2 = 2 3,5-dihydroxy-1,4-naphthoquinone + 2 H2O [RN:R11496]
Reaction(KEGG)
Substrate
juglone [CPD:C03840];
O2 [CPD:C00007];
H2O [CPD:C00001];
naphthalene-1,2,4,8-tetrol [CPD:C21414]
Product
3,5-dihydroxy-1,4-naphthoquinone [CPD:C04110];
naphthalene-1,2,4,8-tetrol [CPD:C21414];
H2O [CPD:C00001]
Comment
Even though oxygen is consumed, molecular oxygen is not incorporated into the product. Catalysis starts by incorporation of an oxygen atom from a water molecule into the substrate. The naphthalene-1,2,4,8-tetrol intermediate is then oxidized by molecular oxygen, which is reduced to water. Also acts on 1,4-naphthoquinone, naphthazarin and 2-chloro-1,4-naphthoquinone.
History
EC 1.17.3.4 created 1989 as EC 1.14.99.27, transferred 2016 to EC 1.17.3.4
Reference
1  [PMID:4041238]
  Authors
Rettenmaier H, Lingens F.
  Title
Purification and some properties of two isofunctional juglone hydroxylases from Pseudomonas putida J1.
  Journal
Biol Chem Hoppe Seyler 366:637-46 (1985)
Other DBs
ExplorEnz - The Enzyme Database: 1.17.3.4
IUBMB Enzyme Nomenclature: 1.17.3.4
ExPASy - ENZYME nomenclature database: 1.17.3.4
BRENDA, the Enzyme Database: 1.17.3.4
CAS: 98865-54-4

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