KEGG   ENZYME: 1.17.5.1
Entry
EC 1.17.5.1                 Enzyme                                 
Name
phenylacetyl-CoA dehydrogenase;
phenylacetyl-CoA:acceptor oxidoreductase
Class
Oxidoreductases;
Acting on CH or CH2 groups;
With a quinone or similar compound as acceptor
Sysname
phenylacetyl-CoA:quinone oxidoreductase
Reaction(IUBMB)
phenylacetyl-CoA + H2O + 2 quinone = phenylglyoxylyl-CoA + 2 quinol [RN:R07222]
Reaction(KEGG)
R07222
Substrate
phenylacetyl-CoA [CPD:C00582];
H2O [CPD:C00001];
quinone [CPD:C15602]
Product
phenylglyoxylyl-CoA [CPD:C15524];
quinol [CPD:C15603]
Comment
The enzyme from Thauera aromatica is a membrane-bound molybdenum---iron---sulfur protein. The enzyme is specific for phenylacetyl-CoA as substrate. Phenylacetate, acetyl-CoA, benzoyl-CoA, propanoyl-CoA, crotonyl-CoA, succinyl-CoA and 3-hydroxybenzoyl-CoA cannot act as substrates. The oxygen atom introduced into the product, phenylglyoxylyl-CoA, is derived from water and not molecular oxygen. Duroquinone, menaquinone and 2,6-dichlorophenolindophenol (DCPIP) can act as acceptor, but the likely physiological acceptor is ubiquinone [1]. A second enzyme, EC 3.1.2.25, phenylacetyl-CoA hydrolase, converts the phenylglyoxylyl-CoA formed into phenylglyoxylate.
History
EC 1.17.5.1 created 2004
Pathway
ec00360  Phenylalanine metabolism
ec01100  Metabolic pathways
Orthology
K18361  phenylacetyl-CoA:acceptor oxidoreductase
Genes
SHDSUTH_01622
EBAebA5393(padB)
ABREpbN1_03670(padB1) pbN1_05780(padB2)
APETToN1_39530(padB)
AZOazo1946(padB)
AOAdqs_2101
AZAAZKH_2904(padB)
AZIAzCIB_2845
ACOMCEW83_03765
AZDCDA09_15510
AZRCJ010_12705
TMZTmz1t_0199 Tmz1t_2959
TCLTchl_0104 Tchl_0789 Tchl_2139
TAKTharo_0098 Tharo_0858 Tharo_1297
DDZDSYM_17570
RPDRPD_1507
MAGamb1241 amb2015
MAGXXM1_1873 XM1_2734
MAGNWV31_17125
 » show all
Reference
1  [PMID:10336636]
  Authors
Rhee SK, Fuchs G
  Title
Phenylacetyl-CoA:acceptor oxidoreductase, a membrane-bound molybdenum-iron-sulfur enzyme involved in anaerobic metabolism of phenylalanine in the denitrifying bacterium Thauera aromatica.
  Journal
Eur J Biochem 262:507-15 (1999)
DOI:10.1046/j.1432-1327.1999.00399.x
Reference
2  [PMID:9575237]
  Authors
Schneider S, Fuchs G.
  Title
Phenylacetyl-CoA:acceptor oxidoreductase, a new alpha-oxidizing enzyme that produces phenylglyoxylate. Assay, membrane localization, and differential production in Thauera aromatica.
  Journal
Arch Microbiol 169:509-16 (1998)
DOI:10.1007/s002030050604
Other DBs
ExplorEnz - The Enzyme Database: 1.17.5.1
IUBMB Enzyme Nomenclature: 1.17.5.1
ExPASy - ENZYME nomenclature database: 1.17.5.1
BRENDA, the Enzyme Database: 1.17.5.1
CAS: 210756-43-7

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