KEGG   ENZYME: 1.17.5.2
Entry
EC 1.17.5.2                 Enzyme                                 
Name
caffeine dehydrogenase
Class
Oxidoreductases;
Acting on CH or CH2 groups;
With a quinone or similar compound as acceptor
Sysname
caffeine:ubiquinone oxidoreductase
Reaction(IUBMB)
caffeine + ubiquinone + H2O = 1,3,7-trimethylurate + ubiquinol [RN:R07980]
Reaction(KEGG)
R07980
Substrate
caffeine [CPD:C07481];
ubiquinone [CPD:C00399];
H2O [CPD:C00001]
Product
1,3,7-trimethylurate [CPD:C16361];
ubiquinol [CPD:C00390]
Comment
This enzyme, characterized from the soil bacterium Pseudomonas sp. CBB1, catalyses the incorporation of an oxygen atom originating from a water molecule into position C-8 of caffeine. It can also use theobromine as substrate. The enzyme utilizes short-tail ubiquinones as the preferred electron acceptor.
History
EC 1.17.5.2 created 2010
Pathway
ec00232  Caffeine metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K21673  caffeine dehydrogenase subunit alpha
K21674  caffeine dehydrogenase subunit beta
K21675  caffeine dehydrogenase subunit gamma
Reference
1  [PMID:17981969]
  Authors
Yu CL, Kale Y, Gopishetty S, Louie TM, Subramanian M
  Title
A novel caffeine dehydrogenase in Pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid.
  Journal
J Bacteriol 190:772-6 (2008)
DOI:10.1128/JB.01390-07
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.17.5.2
IUBMB Enzyme Nomenclature: 1.17.5.2
ExPASy - ENZYME nomenclature database: 1.17.5.2
BRENDA, the Enzyme Database: 1.17.5.2

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