| Entry |
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| Name |
4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase;
isopentenyl-diphosphate:NADP+ oxidoreductase;
LytB;
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase;
HMBPP reductase;
IspH;
LytB/IspH
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| Class |
Oxidoreductases;
Acting on CH or CH2 groups;
With an iron-sulfur protein as acceptor
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| Sysname |
isopentenyl-diphosphate:ferredoxin oxidoreductase
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| Reaction(IUBMB) |
(1) isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ [RN: R05884];
(2) dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ [RN: R08210]
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| Reaction(KEGG) |
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| Substrate |
isopentenyl diphosphate [CPD: C00129];
oxidized ferredoxin [iron-sulfur] cluster [CPD: C00139];
H2O [CPD: C00001];
dimethylallyl diphosphate [CPD: C00235]
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| Product |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate [CPD: C11811];
reduced ferredoxin [iron-sulfur] cluster [CPD: C00138];
H+ [CPD: C00080]
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| Comment |
An iron-sulfur protein that contains either a [3Fe-4S] [6] or a [4Fe-4S] [5] cluster. This enzyme forms a system with a ferredoxin or a flavodoxin and an NAD(P)H-dependent reductase. This is the last enzyme in the non-mevalonate pathway for isoprenoid biosynthesis. This pathway, also known as the 1-deoxy-D-xylulose 5-phosphate (DOXP) or as the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway, is found in most bacteria and in plant chloroplasts. The enzyme acts in the reverse direction, producing a 5:1 mixture of isopentenyl diphosphate and dimethylallyl diphosphate.
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| History |
EC 1.17.7.4 created 2003 as EC 1.17.1.2, modified 2009, transferred 2016 to EC 1.17.7.4
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| Pathway |
| ec00900 | Terpenoid backbone biosynthesis |
| ec01110 | Biosynthesis of secondary metabolites |
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| Orthology |
| K03527 | 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase |
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| Genes |
» show all
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| Reference |
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| Authors |
Rohdich F, Hecht S, Gartner K, Adam P, Krieger C, Amslinger S, Arigoni D, Bacher A, Eisenreich W |
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| Title |
Studies on the nonmevalonate terpene biosynthetic pathway: metabolic role of IspH (LytB) protein. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Hintz M, Reichenberg A, Altincicek B, Bahr U, Gschwind RM, Kollas AK, Beck E, Wiesner J, Eberl M, Jomaa H. |
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| Title |
Identification of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate as a major activator for human gammadelta T cells in Escherichia coli. |
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| Journal |
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| Reference |
3 |
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| Authors |
Charon, L., Pale-Grosdemange, C. and Rohmer, M. |
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| Title |
On the reduction steps in the mevalonate independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis in the bacterium Zymomonas mobilis. |
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| Journal |
Tetrahedron Lett 40:7231-7234 (1999) |
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| Reference |
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| Authors |
Rohrich RC, Englert N, Troschke K, Reichenberg A, Hintz M, Seeber F, Balconi E, Aliverti A, Zanetti G, Kohler U, Pfeiffer M, Beck E, Jomaa H, Wiesner J |
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| Title |
Reconstitution of an apicoplast-localised electron transfer pathway involved in the isoprenoid biosynthesis of Plasmodium falciparum. |
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| Journal |
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| Reference |
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| Authors |
Wolff M, Seemann M, Tse Sum Bui B, Frapart Y, Tritsch D, Garcia Estrabot A, Rodriguez-Concepcion M, Boronat A, Marquet A, Rohmer M |
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| Title |
Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Grawert T, Kaiser J, Zepeck F, Laupitz R, Hecht S, Amslinger S, Schramek N, Schleicher E, Weber S, Haslbeck M, Buchner J, Rieder C, Arigoni D, Bacher A, Eisenreich W, Rohdich F |
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| Title |
IspH protein of Escherichia coli: studies on iron-sulfur cluster implementation and catalysis. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 1.17.7.4 |
| ExPASy - ENZYME nomenclature database: | 1.17.7.4 |
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