KEGG   ENZYME: 1.17.9.2
Entry
EC 1.17.9.2                 Enzyme                                 

Name
(+)-pinoresinol hydroxylase;
pinoresinol alpha-hydroxylase;
pinAB (gene names)
Class
Oxidoreductases;
Acting on CH or CH2 groups
Sysname
(+)-pinoresinol:azurin oxidoreductase
Reaction(IUBMB)
(+)-pinoresinol + 2 oxidized azurin + H2O = (+)-6-hydroxypinoresinol + 2 reduced azurin + 2 H+ [RN:R12653]
Reaction(KEGG)
R12653
Substrate
(+)-pinoresinol [CPD:C05366];
oxidized azurin [CPD:C05357];
H2O [CPD:C00001]
Product
(+)-6-hydroxypinoresinol [CPD:C22302];
reduced azurin [CPD:C05358];
H+ [CPD:C00080]
Comment
Contains FAD. This enzyme, characterized from the bacterium Pseudomonas sp. SG-MS2, catalyses the incorporation of an oxygen atom originating from a water molecule into position C-6 of the lignan (+)-pinoresinol. The enzyme consists of a flavoprotein subunit and a c-type cytochrome subunit. Electrons that originate in the substrate are transferred via the FAD cofactor and the cytochrome subunit to the blue-copper protein azurin.
History
EC 1.17.9.2 created 2020
Orthology
K24452  (+)-pinoresinol hydroxylase
K24453  (+)-pinoresinol hydroxylase
Genes
RBAD: H2866_00265
XHY: FZ025_04795 FZ025_04800
PCQ: PcP3B5_26530(petJ) PcP3B5_26540(pchF_3)
PALK: PSAKL28_30910 PSAKL28_30920
BGE: BC1002_4150 BC1002_4151
EBA: ebA299(pchC) ebA300(pchF)
TCL: Tchl_1573 Tchl_1574
TAK: Tharo_2929 Tharo_2930
HWI: A0Z60_01365 A0Z60_01370
CCOL: BN865_03820 BN865_03830
CCQ: N149_0007
CCY: YSS_00040
CCOF: VC76_00025(pchF_1) VC76_00030(pchF_2) VC76_00040
APOC: APORC_1778
AAY: WYH_03116(pchF_3) WYH_03118(petJ_3) WYH_03119(pchF_4)
 » show all
Reference
1  [PMID:32198167]
  Authors
Shettigar M, Balotra S, Kasprzak A, Pearce SL, Lacey MJ, Taylor MC, Liu JW, Cahill D, Oakeshott JG, Pandey G
  Title
Oxidative Catabolism of (+)-Pinoresinol Is Initiated by an Unusual Flavocytochrome Encoded by Translationally Coupled Genes within a Cluster of (+)-Pinoresinol-Coinduced Genes in Pseudomonas sp. Strain SG-MS2.
  Journal
Appl Environ Microbiol 86:e00375-20 (2020)
DOI:10.1128/AEM.00375-20
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.17.9.2
IUBMB Enzyme Nomenclature: 1.17.9.2
ExPASy - ENZYME nomenclature database: 1.17.9.2
BRENDA, the Enzyme Database: 1.17.9.2

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