KEGG   ENZYME: 1.17.99.10
Entry
EC 1.17.99.10               Enzyme                                 

Name
steroid C-25 hydroxylase;
s25dA1 (gene name);
s25dA1B3 (gene name);
s25dA1C3 (gene name);
cholesterol C-25 dehydrogenase;
steroid C-25 dehydrogenase
Class
Oxidoreductases;
Acting on CH or CH2 groups;
With unknown physiological acceptors
Sysname
cholest-4-en-3-one:acceptor oxidoreductase (25-hydroxylating)
Reaction(IUBMB)
cholest-4-en-3-one + acceptor + H2O = 25-hydroxycholest-4-en-3-one + reduced acceptor [RN:R12677]
Reaction(KEGG)
R12677
Substrate
cholest-4-en-3-one [CPD:C00599];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Product
25-hydroxycholest-4-en-3-one [CPD:C22325];
reduced acceptor [CPD:C00030]
Comment
The enzyme, characterized from the bacterium Sterolibacterium denitrificans, participates in the anaerobic degradation of cholesterol. The enzyme can accept several substrates including vitamin D3. The enzyme contains a bis(guanylyl molybdopterin) cofactor, five [Fe-S] clusters, and one heme b. cf. EC 1.14.99.38, cholesterol 25-monooxygenase, an oxygen-requiring eukaryotic enzyme that catalyses a similar transformation.
History
EC 1.17.99.10 created 2020
Orthology
K24713  steroid C-25 hydroxylase alpha subunit
K24714  steroid C-25 hydroxylase beta subunit
Genes
SDF: ACG33_10675 ACG33_10685
GBI: PG2T_03370 PG2T_03375
GPB: HDN1F_20090(ddhA) HDN1F_20100(ddhB)
DOE: DENOEST_1434(s25dA) DENOEST_1435(ddhB) DENOEST_2077(narH) DENOEST_3311(serB) DENOEST_3312(s25dA) DENOEST_3335(narH) DENOEST_3461(serB)
EBA: ebA5790(ebdA2) ebA5791(ebdB2)
AZD: CDA09_09000 CDA09_09005
DOL: Dole_0194 Dole_0196
Reference
1  [PMID:22942275]
  Authors
Dermer J, Fuchs G.
  Title
Molybdoenzyme that catalyzes the anaerobic hydroxylation of a tertiary carbon atom in the side chain of cholesterol.
  Journal
J Biol Chem 287:36905-16 (2012)
DOI:10.1074/jbc.M112.407304
  Sequence
Reference
2  [PMID:27726023]
  Authors
Rugor A, Tataruch M, Staron J, Dudzik A, Niedzialkowska E, Nowak P, Hogendorf A, Michalik-Zym A, Napruszewska DB, Jarzebski A, Szymanska K, Bialas W, Szaleniec M.
  Title
Regioselective hydroxylation of cholecalciferol, cholesterol and other sterol derivatives by steroid C25 dehydrogenase.
  Journal
Appl Microbiol Biotechnol 101:1163-1174 (2017)
DOI:10.1007/s00253-016-7880-2
Reference
3  [PMID:28482186]
  Authors
Rugor A, Wojcik-Augustyn A, Niedzialkowska E, Mordalski S, Staron J, Bojarski A, Szaleniec M.
  Title
Reaction mechanism of sterol hydroxylation by steroid C25 dehydrogenase - Homology model, reactivity and isoenzymatic diversity.
  Journal
J Inorg Biochem 173:28-43 (2017)
DOI:10.1016/j.jinorgbio.2017.04.027
Reference
4  [PMID:29921665]
  Authors
Jacoby C, Eipper J, Warnke M, Tiedt O, Mergelsberg M, Stark HJ, Daus B, Martin-Moldes Z, Zamarro MT, Diaz E, Boll M.
  Title
Four Molybdenum-Dependent Steroid C-25 Hydroxylases: Heterologous Overproduction, Role in Steroid Degradation, and Application for 25-Hydroxyvitamin D3 Synthesis.
  Journal
mBio 9:e00694-18 (2018)
DOI:10.1128/mBio.00694-18
Other DBs
ExplorEnz - The Enzyme Database: 1.17.99.10
IUBMB Enzyme Nomenclature: 1.17.99.10
ExPASy - ENZYME nomenclature database: 1.17.99.10
BRENDA, the Enzyme Database: 1.17.99.10

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