KEGG   ENZYME: 1.17.99.11
Entry
EC 1.17.99.11               Enzyme                                 

Name
3-oxo-Delta1-steroid hydratase/dehydrogenase;
atcABC (gene names)
Class
Oxidoreductases;
Acting on CH or CH2 groups;
With unknown physiological acceptors
Sysname
3-oxo-Delta1-steroid:acceptor 1-oxidoreductase
Reaction(IUBMB)
a 3-oxo-Delta1-steroid + H2O + acceptor = a steroid 1,3-dione + reduced acceptor (overall reaction) [RN:R12678];
(1a) a 3-oxo-Delta1-steroid + H2O = a 1-hydroxy-3-oxo-steroid [RN:R12679];
(1b) a 1-hydroxy-3-oxo-steroid + acceptor = a steroid 1,3-dione + reduced acceptor [RN:R12680]
Reaction(KEGG)
Substrate
3-oxo-Delta1-steroid [CPD:C02941];
H2O [CPD:C00001];
acceptor [CPD:C00028];
1-hydroxy-3-oxo-steroid [CPD:C22327]
Product
steroid 1,3-dione [CPD:C22326];
reduced acceptor [CPD:C00030];
1-hydroxy-3-oxo-steroid [CPD:C22327]
Comment
A molybdenum enzyme. The enzyme, characterized from the bacterium Steroidobacter denitrificans, is involved in the anaetrobic degradation of steroids. It is specific to 3-oxo-Delta1-steroids such as androsta-1-ene-3,17-dione and Delta1-dihydrotestosterone and does not act on 3-oxo-Delta4-steroids.
History
EC 1.17.99.11 created 2020
Orthology
K24716  3-oxo-Delta1-steroid hydratase/dehydrogenase large subunit
K24717  3-oxo-Delta1-steroid hydratase/dehydrogenase medium subunit
K24718  3-oxo-Delta1-steroid hydratase/dehydrogenase small subunit
Genes
SDF: ACG33_03370 ACG33_03375 ACG33_03390
DOE: DENOEST_2087 DENOEST_2088 DENOEST_2089(cutS) DENOEST_2108 DENOEST_2110(cutS) DENOEST_2111(cutM) DENOEST_3658
AZI: AzCIB_1523 AzCIB_1524 AzCIB_1525
AZD: CDA09_10040 CDA09_10045 CDA09_10050
Reference
1  [PMID:26872041]
  Authors
Yang FC, Chen YL, Tang SL, Yu CP, Wang PH, Ismail W, Wang CH, Ding JY, Yang CY, Yang CY, Chiang YR.
  Title
Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment.
  Journal
ISME J 10:1967-83 (2016)
DOI:10.1038/ismej.2015.255
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.17.99.11
IUBMB Enzyme Nomenclature: 1.17.99.11
ExPASy - ENZYME nomenclature database: 1.17.99.11
BRENDA, the Enzyme Database: 1.17.99.11

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