KEGG   ENZYME: 1.17.99.8
Entry
EC 1.17.99.8                Enzyme                                 

Name
limonene dehydrogenase;
ctmAB (gene names)
Class
Oxidoreductases;
Acting on CH or CH2 groups;
With unknown physiological acceptors
Sysname
limonene:acceptor oxidoreductase (7-hydroxylating)
Reaction(IUBMB)
(1) (S)-limonene + H2O + acceptor = (-)-perillyl alcohol + reduced acceptor [RN:R12591];
(2) (R)-limonene + H2O + acceptor = (+)-perillyl alcohol + reduced acceptor [RN:R12592]
Reaction(KEGG)
R12591 R12592
Substrate
(S)-limonene [CPD:C00521];
H2O [CPD:C00001];
acceptor [CPD:C00028];
(R)-limonene [CPD:C06099]
Product
(-)-perillyl alcohol [CPD:C02452];
reduced acceptor [CPD:C00030];
(+)-perillyl alcohol [CPD:C22272]
Comment
Contains FAD. The enzyme, characterized from the bacterium Castellaniella defragrans 65Phen, hydroxylates the R- and S-enantiomers at a similar rate. The in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF).
History
EC 1.17.99.8 created 2020
Orthology
K24122  limonene dehydrogenase
Genes
CDN: BN940_14221 BN940_14226
Reference
1  [PMID:24952578]
  Authors
Petasch J, Disch EM, Markert S, Becher D, Schweder T, Huttel B, Reinhardt R, Harder J
  Title
The oxygen-independent metabolism of cyclic monoterpenes in Castellaniella defragrans 65Phen.
  Journal
BMC Microbiol 14:164 (2014)
DOI:10.1186/1471-2180-14-164
  Sequence
Reference
2  [PMID:29716998]
  Authors
Puentes-Cala E, Liebeke M, Markert S, Harder J
  Title
Limonene dehydrogenase hydroxylates the allylic methyl group of cyclic monoterpenes in the anaerobic terpene degradation by Castellaniella defragrans.
  Journal
J Biol Chem 293:9520-9529 (2018)
DOI:10.1074/jbc.RA117.001557
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.17.99.8
IUBMB Enzyme Nomenclature: 1.17.99.8
ExPASy - ENZYME nomenclature database: 1.17.99.8
BRENDA, the Enzyme Database: 1.17.99.8

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