KEGG   ENZYME: 1.18.6.1Help
Entry
EC 1.18.6.1                 Enzyme                                 

Name
nitrogenase;
reduced ferredoxin:dinitrogen oxidoreductase (ATP-hydrolysing)
Class
Oxidoreductases;
Acting on iron-sulfur proteins as donors;
With dinitrogen as acceptor
BRITE hierarchy
Sysname
ferredoxin:dinitrogen oxidoreductase (ATP-hydrolysing, molybdenum-dependent)
Reaction(IUBMB)
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate [RN:R05185]
Reaction(KEGG)
Substrate
reduced ferredoxin [CPD:C00138];
H+ [CPD:C00080];
N2 [CPD:C00697];
ATP [CPD:C00002];
H2O [CPD:C00001]
Product
oxidized ferredoxin [CPD:C00139];
H2 [CPD:C00282];
NH3 [CPD:C00014];
ADP [CPD:C00008];
phosphate [CPD:C00009]
Comment
Requires Mg2+. The enzyme is a complex of two components (namely dinitrogen reductase and dinitrogenase). Dinitrogen reductase is a [4Fe-4S] protein, which, in the presence of two molecules of ATP, transfers an electron from ferredoxin to the dinitrogenase component. Dinitrogenase is a molybdenum-iron protein that reduces dinitrogen to two molecules of ammonia in three successive two-electron reductions via diazene and hydrazine. The reduction is initiated by formation of hydrogen in stoichiometric amounts [2]. Acetylene is reduced to ethylene (but only very slowly to ethane), azide to nitrogen and ammonia, and cyanide to methane and ammonia. In the absence of a suitable substrate, hydrogen is slowly formed. Ferredoxin may be replaced by flavodoxin [see EC 1.19.6.1 nitrogenase (flavodoxin)]. The enzyme does not reduce CO (cf. EC 1.18.6.2, vanadium-dependent nitrogenase).
History
EC 1.18.6.1 created 1978 as EC 1.18.2.1, transferred 1984 to EC 1.18.6.1, modified 2005, modified 2018
Pathway
ec00625  Chloroalkane and chloroalkene degradation
ec00910  Nitrogen metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K00531  nitrogenase delta subunit
K02586  nitrogenase molybdenum-iron protein alpha chain
K02588  nitrogenase iron protein NifH
K02591  nitrogenase molybdenum-iron protein beta chain
Genes
ENR: H650_03210(nifH) H650_03215 H650_03220
ENF: AKI40_3044 AKI40_3045 AKI40_3046
KPK: A593_02115(nifH) A593_02120 A593_02125
KPV: KPNIH29_17525(nifH) KPNIH29_17530 KPNIH29_17535
KPE: KPK_1712(nifK) KPK_1713(nifD) KPK_1714(nifH)
KVA: Kvar_1601 Kvar_1602 Kvar_1603
KVD: KR75_26120 KR75_26125 KR75_26130
KVQ: SP68_06265 SP68_06270 SP68_06275
KOX: KOX_24920 KOX_24925 KOX_24930
KOE: A225_3835 A225_3836 A225_3837
KQV: B8P98_09570(nifK) B8P98_09575(nifD) B8P98_09580(nifH)
KRD: A3780_10720(nifK) A3780_10725(nifD) A3780_10730(nifH) A3780_13145(anfK) A3780_13150(anfG) A3780_13155(anfD) A3780_13160(nifH)
EBC: C2U52_22725(nifK) C2U52_22730(nifD) C2U52_22735(nifH)
ECA: ECA2954(nifK) ECA2955(nifD) ECA2956(nifH)
PATO: GZ59_29580(nifK) GZ59_29590(nifD) GZ59_29600(nifH)
DDD: Dda3937_02155(nifH) Dda3937_02156(nifD) Dda3937_02157(nifK)
DFN: CVE23_19775(nifK) CVE23_19780(nifD) CVE23_19785(nifH)
PSA: PST_1326(nifH) PST_1327(nifD) PST_1328(nifK)
PSR: PSTAA_1358(nifH) PSTAA_1359(nifD) PSTAA_1360(nifK)
AVN: Avin_01380(nifH) Avin_01390(nifD) Avin_01400(nifK) Avin_02590(vnfK) Avin_02600(vnfG) Avin_02610(vnfD) Avin_02660(vnfH) Avin_48970(anfK) Avin_48980(anfG) Avin_48990(anfD) Avin_49000(anfH)
AVL: AvCA_01380(nifH) AvCA_01390(nifD) AvCA_01400(nifK) AvCA_02590(vnfK) AvCA_02600(vnfG) AvCA_02610(vnfD) AvCA_02660(vnfH) AvCA_48970(anfK) AvCA_48980(anfG) AvCA_48990(anfD) AvCA_49000(anfH)
AVD: AvCA6_01380(nifH) AvCA6_01390(nifD) AvCA6_01400(nifK) AvCA6_02590(vnfK) AvCA6_02600(vnfG) AvCA6_02610(vnfD) AvCA6_02660(vnfH) AvCA6_48970(anfK) AvCA6_48980(anfG) AvCA6_48990(anfD) AvCA6_49000(anfH)
ACX: Achr_1260(nifH) Achr_1270(nifD) Achr_1280(nifK) Achr_2510(vnfK) Achr_2520(vnfG) Achr_2530(vnfD) Achr_2560(vnfH)
TTU: TERTU_1537(nifH) TERTU_1538(nifD) TERTU_1539(nifK)
MCA: MCA0229(nifH) MCA0230(nifD) MCA0231(nifK)
MPSY: CEK71_01050(nifH) CEK71_01055(nifD) CEK71_01060(nifK)
CTI: pRALTA_0390(nifH) pRALTA_0391(nifD) pRALTA_0392(nifK)
BVE: AK36_5492(nifH) AK36_5493(nifD) AK36_5494(nifK)
BXE: Bxe_B1465(nifH) Bxe_B1466(nifD) Bxe_B1467(nifK)
BXB: DR64_6772(nifH) DR64_6773(nifD) DR64_6774(nifK)
PARB: CJU94_32990(nifD) CJU94_32995(nifH) CJU94_36080(nifK)
HSE: Hsero_2851(nifK) Hsero_2852(nifD) Hsero_2853(nifH)
HRB: Hrubri_2375(nifK) Hrubri_2376(nifD) Hrubri_2377(nifH)
RGE: RGE_25430(nifH) RGE_25440(nifD) RGE_25450(nifK) RGE_42880(nifH) RGE_42890(nifD) RGE_42900(nifK)
METR: BSY238_1221(nifH) BSY238_1222(nifD) BSY238_1223(nifK)
AZO: azo0538(nifH) azo0539(nifD) azo0540(nifK)
AZA: AZKH_0789(nifK) AZKH_0790(nifD) AZKH_0791(nifH) AZKH_4078(nifH) AZKH_4079(nifD) AZKH_4080(nifK)
ACOM: CEW83_10465(nifK) CEW83_10470(nifD) CEW83_10475(nifH)
ZPA: C3497_06300(nifK) C3497_06305(nifD) C3497_06310(nifH)
WSU: WS1391(NIFK) WS1392(NIFD) WS1394(NIFH)
SMUL: SMUL_1285(nifH) SMUL_1287(nifD) SMUL_1288(nifK) SMUL_1652(anfK) SMUL_1653(anfG) SMUL_1654(anfD)
GSU: GSU2819(nifK) GSU2820(nifD) GSU2821(nifH)
GSK: KN400_2758(nifK) KN400_2759(nifD) KN400_2760(nifH)
GME: Gmet_0662(nifH) Gmet_0663(nifD) Gmet_0664(nifK)
GBM: Gbem_1731 Gbem_2074(nifH) Gbem_2075(nifD) Gbem_2076(nifK)
PCA: Pcar_2098(nifH) Pcar_2099(nifD) Pcar_2100(nifK)
DES: DSOUD_2560(nifK) DSOUD_2561(nifD) DSOUD_2562(nifH)
DVU: DVUA0011(nifK) DVUA0012(nifD) DVUA0015(nifH)
DMA: DMR_20520(nifK) DMR_20530(nifD) DMR_20560(nifH)
DPI: BN4_20068(nifH) BN4_20071(nifD) BN4_20072(nifK)
PPRF: DPRO_3403(nifH) DPRO_3406(nifD) DPRO_3407(nifK)
DAT: HRM2_09670(nifH2) HRM2_09700(nifD2) HRM2_09710(nifK1)
SME: SMa0825(nifH) SMa0827(nifD) SMa0829(nifK)
SMX: SM11_pC1123(nifK) SM11_pC1124(nifD) SM11_pC1125(nifH)
SMI: BN406_04237(nifH) BN406_04238(nifD1) BN406_04239(nifK1)
SMEL: SM2011_a0825(nifH) SM2011_a0827(nifD) SM2011_a0829(nifK)
RHI: NGR_a00870(nifK2) NGR_a00880(nifD2) NGR_a00890(nifH) NGR_a01110(nifK1) NGR_a01120(nifD1) NGR_a01130(nifH)
REP: IE4803_PB00349(nifK-1) IE4803_PB00350(nifD-1) IE4803_PB00351(nifH-1) IE4803_PB00410(nifH-2) IE4803_PB00460(nifK-2) IE4803_PB00461(nifD-2) IE4803_PB00462(nifH-3)
RLE: pRL100160(nifK) pRL100161(nifD) pRL100162(nifH)
RIR: BN877_p0283(nifK) BN877_p0284(nifD) BN877_p0285(nifH)
RHN: AMJ98_PC00260(nifK-1) AMJ98_PC00261(nifD-1) AMJ98_PC00262(nifH-1) AMJ98_PC00312(nifH-2) AMJ98_PC00355(nifK-2) AMJ98_PC00356(nifD-2) AMJ98_PC00357(nifH-3)
RPHA: AMC79_PC00237(nifK-1) AMC79_PC00238(nifD-1) AMC79_PC00239(nifH-1) AMC79_PC00288(nifH-2) AMC79_PC00326(nifK-2) AMC79_PC00327(nifD-2) AMC79_PC00328(nifH-3)
RHX: AMK02_PC00255(nifK-1) AMK02_PC00256(nifD-1) AMK02_PC00257(nifH-1) AMK02_PC00306(nifH-2) AMK02_PC00344(nifK-2) AMK02_PC00345(nifD-2) AMK02_PC00346(nifH-3)
NGG: RG540_PA10420(nifH) RG540_PA10430(nifD) RG540_PA10440(nifK)
BJA: blr1743(nifD) blr1744(nifK) blr1769(nifH)
BJU: BJ6T_80500(nifH) BJ6T_80750(nifK) BJ6T_80760(nifD)
BRA: BRADO5394(nifH) BRADO5438(nifK) BRADO5439(nifD) BRADO5440(nifH)
BBT: BBta_5879(nifH) BBta_5923(nifK) BBta_5924(nifD) BBta_5925(nifH)
BRS: S23_46020 S23_46420(nifK) S23_46430(nifD) S23_46440(nifH)
BRO: BRAD285_1795(nifH) BRAD285_1796(nifD) BRAD285_1797(nifK) BRAD285_1840(nifH)
RPA: RPA1376(vnfH) RPA1378(vnfD) RPA1379(vnfG) RPA1380(vnfK) RPA1435(anfK) RPA1436(anfG) RPA1437(anfD) RPA1438(anfH) RPA2348 RPA2353(nifH1) RPA2363(NifD1) RPA2615(nifH2) RPA2617 RPA2635(nifH3) RPA2636(NifD2) RPA4618(nifK) RPA4619(nifD) RPA4620(nifH4)
AZC: AZC_1039(nifK) AZC_1040(nifD) AZC_1041(nifH2) AZC_3443(nifH1)
HMC: HYPMC_3637(nifH) HYPMC_3685(nifK) HYPMC_3686(nifD) HYPMC_3687(nifH)
MSC: BN69_2623(nifH) BN69_2624(nifD) BN69_2625 BN69_2665(nifH)
HDI: HDIA_4341(nifK1) HDIA_4342(nifD) HDIA_4343(nifH)
RSP: RSP_0539(nifK) RSP_0540(nifD) RSP_0541(nifH)
SAGU: CDO87_16530(nifK) CDO87_16535(nifD) CDO87_16540(nifH)
ZMC: A265_01353 A265_01354(nifE) A265_01355(nifH)
ZMR: A254_01352 A254_01353(nifE) A254_01354(nifH)
GDI: GDI0436(nifH) GDI0437(nifD) GDI0438(nifK)
RCE: RC1_3681(nifK) RC1_3682(nifD) RC1_3683(nifH)
MGY: MGMSRv2__0343(nifK) MGMSRv2__0344(nifD) MGMSRv2__0345(nifH)
MAGX: XM1_3938(nifH) XM1_3939(nifD) XM1_3940(nifK)
AZL: AZL_007690(nifK) AZL_007700(nifD) AZL_007710(nifH)
ALI: AZOLI_0679(nifK) AZOLI_0680(nifD) AZOLI_0681(nifH)
AZT: TSH58p_07635(nifH) TSH58p_07640(nifD) TSH58p_07645(nifK)
NAO: Y958_05330(nifH) Y958_05335(nifD) Y958_05340(nifK)
AFR: AFE_1520(nifK) AFE_1521(nifD) AFE_1522(nifH)
PRI: PRIO_2702(nifH3) PRIO_2703(anfD) PRIO_2704 PRIO_2705(anfK) PRIO_3901 PRIO_3902 PRIO_3908(nifH2) PRIO_5538(nifK) PRIO_5539(nifD) PRIO_5540(nifH1)
PDH: B9T62_25635(nifK) B9T62_25640(nifD) B9T62_25645(nifH)
KYR: CVV65_08110(nifH) CVV65_08115(nifD) CVV65_08120(nifK)
CAC: CA_C0253(nifH) CA_C0256(nifD) CA_C0257(nifK)
CAE: SMB_G0258(nifH) SMB_G0261(nifD) SMB_G0262(nifK)
CAY: CEA_G0259(nifH) CEA_G0262(nifD) CEA_G0263(nifK)
CBO: CBO0690(nifH)
CBA: CLB_0729(nifH)
CBH: CLC_0744(nifH)
CBY: CLM_0807(nifH)
CBL: CLK_0091(nifH)
CBB: CLD_0076(nifH)
CBI: CLJ_B0762(nifH)
CBF: CLI_0759(nifH)
CBM: CBF_0726(nifH)
CKL: CKL_0370(anfD) CKL_0371(anfG) CKL_0372(anfK) CKL_0825(nifD1) CKL_0826(nifK1) CKL_1033(nifH1) CKL_1034(nifD2) CKL_1035(nifK2) CKL_1042(nifD3) CKL_1043(nifD4) CKL_1044(nifK3) CKL_1045(nifH2) CKL_1048(nifK4) CKL_1745(vnfK) CKL_1746(vnfG) CKL_1747(vnfD) CKL_1748(vnfH) CKL_2461(nifH3) CKL_3077(nifK7) CKL_3078(nifD10) CKL_3081(nifH4)
CLJ: CLJU_c04900(nifH1) CLJU_c04930(nifD1) CLJU_c04940(nifK1) CLJU_c23050(nifH2) CLJU_c23120(nifH3) CLJU_c23510(nifK2) CLJU_c23520(nifD2) CLJU_c23530(nifH4)
CSR: Cspa_c14120(nifE1) Cspa_c14130(nifK1) Cspa_c14150(nifD1) Cspa_c14160(nifK2) Cspa_c14260(nifH1) Cspa_c30750(nifH2) Cspa_c30780(nifD2) Cspa_c30790(nifK3) Cspa_c30870(nifH3) Cspa_c30880(anfD) Cspa_c30890(anfG) Cspa_c30900(anfK)
CLD: CLSPO_c07130(nifH)
CACE: CACET_c13970(nifH) CACET_c14000(nifD) CACET_c14010(nifK1)
AOE: Clos_0309
CSS: Cst_c19140(nifH1) Cst_c19800(nifE) Cst_c19810(nifK) Cst_c19880(nifH2)
RUS: RBI_I00176(nifH1) RBI_I00179(nifH2)
SWO: Swol_0408
DED: DHBDCA_p2004(nifK) DHBDCA_p2005(nifD) DHBDCA_p2006(nifH)
DEC: DCF50_p2016(nifK) DCF50_p2017(nifD) DCF50_p2018(nifH)
HMO: HM1_0864(nifK) HM1_0865(nifD) HM1_0866(nifH)
ELM: ELI_1717
AWO: Awo_c00100(nifH1) Awo_c06960(anfK) Awo_c06970(anfG) Awo_c06980(anfD) Awo_c07010(anfH) Awo_c07900(nifK) Awo_c07910(nifD) Awo_c07940(nifH2)
MTA: Moth_0551
TPZ: Tph_c08650(nifH) Tph_c08680(nifD1) Tph_c08690(nifK)
TACI: TDSAC_1033
CAD: Curi_c03680(nifH1) Curi_c21590(nifK1) Curi_c21600(nifE3) Curi_c21610(nifH2) Curi_c21640(nifD) Curi_c21650(nifK2)
SRI: SELR_27780(nifK) SELR_27790(nifE) SELR_27800(nifH)
FAL: FRAAL6811(nifK) FRAAL6812(nifD) FRAAL6813(nifH)
GPA: GPA_17780
CYA: CYA_1821(nifK) CYA_1823(nifD) CYA_1824(nifH)
CYB: CYB_0421(nifH) CYB_0422(nifD) CYB_0423(nifK)
CYT: cce_0559(nifH) cce_0560(nifD) cce_0561(nifK)
ANA: all1440(nifK) all1454(nifD) all1455(nifH) alr0874(nifH2)
NOE: CLI64_00360(nifD) CLI64_00480(nifK)
ANB: ANA_C13419(nifH) ANA_C13511(nifD) ANA_C13533(nifK)
NSP: BMF81_03005(nifH) BMF81_04093(nifK) BMF81_04097(nifD) BMF81_04098(nifH_2) BMF81_04106(nifH_3)
CEO: ETSB_1470(nifK) ETSB_1471(nifD) ETSB_1472(nifH)
DET: DET1154(nifK) DET1155(nifD) DET1158(nifH)
MIN: Minf_1873(nifD) Minf_1874(nifD) Minf_1876(nifH)
EPO: Epro_0685(nifD) Epro_0686(nifK) Epro_0693(nifH)
FNU: FN0303
CTE: CT1533(nifH) CT1536(nifD) CT1537(nifK)
PROC: Ptc2401_01795(nifK_2) Ptc2401_01796(nifD_2) Ptc2401_01799(nifH1_2)
PROS: CHL67_07895(nifH) CHL67_07910(nifD) CHL67_07915(nifK)
HTH: HTH_1136(nifH)
TYE: THEYE_A1715(nifK) THEYE_A1716(nifD) THEYE_A1717(nifH)
LFC: LFE_2396(nifH) LFE_2397(nifD) LFE_2398(nifK)
MJA: MJ_0879
MMP: MMP0147 MMP0853(nifH) MMP0856(nifD) MMP0857(nifK)
MVO: Mvol_0700
MAC: MA_1205(anfH) MA_1208(anfK) MA_1209(anfG) MA_1210(anfD) MA_1213(vnfH) MA_1216(vnfD) MA_1217(vnfG) MA_1218(vnfK) MA_1633(nifH) MA_3895(nifH) MA_3898(nifD) MA_3899(nifK)
MPY: Mpsy_1341(nifH) Mpsy_1344(nifD) Mpsy_1345(nifK)
MCJ: MCON_0607(nifH2) MCON_0610(nifD2) MCON_0611(nifK)
MHU: Mhun_0793
MBG: BN140_0328(nifH)
MPD: MCP_0905(nifH-2)
MEZ: Mtc_0142(nifH-2) Mtc_0145(nifD) Mtc_0146(nifK)
RCI: RCIX1606(nifH-1) RCIX1611(nifD) RCIX1613(nifK)
MMG: MTBMA_c01460(nifH1) MTBMA_c01490(nifD) MTBMA_c01500(nifK1) MTBMA_c01520(nifN)
MWO: MWSIV6_0101(nifH) MWSIV6_0104(nifD3) MWSIV6_0105(nifD5) MWSIV6_0106(nifD7) MWSIV6_0107(nifK1)
METE: tca_01509(nifH1_2) tca_01512(vnfD) tca_01513(nifK_1) tca_01515(nifK_2)
MFC: BRM9_0192(nifN) BRM9_0194(nifK) BRM9_0195(nifD) BRM9_0198(nifH)
MCUB: MCBB_1935(nifK2) MCBB_1937(nifK) MCBB_1938(nifD) MCBB_1941(nifH1)
MKA: MK1416(nifH)
MARC: AR505_0359
 » show all
Taxonomy
Reference
1  [PMID:4390523]
  Authors
Zumft WG, Paneque A, Aparicio PJ, Losada M.
  Title
Mechanism of nitrate reduction in Chlorella.
  Journal
Biochem Biophys Res Commun 36:980-6 (1969)
DOI:10.1016/0006-291X(69)90300-3
Reference
2  [PMID:3200830]
  Authors
Liang J, Burris RH.
  Title
Hydrogen burst associated with nitrogenase-catalyzed reactions.
  Journal
Proc Natl Acad Sci U S A 85:9446-50 (1988)
DOI:10.1073/pnas.85.24.9446
Reference
3  [PMID:15382920]
  Authors
Dance I.
  Title
The mechanism of nitrogenase. Computed details of the site and geometry of binding of alkyne and alkene substrates and intermediates.
  Journal
J Am Chem Soc 126:11852-63 (2004)
DOI:10.1021/ja0481070
Reference
4  [PMID:10852721]
  Authors
Chan JM, Wu W, Dean DR, Seefeldt LC.
  Title
Construction and characterization of a heterodimeric iron protein: defining roles for adenosine triphosphate in nitrogenase catalysis.
  Journal
Biochemistry 39:7221-8 (2000)
DOI:10.1021/bi000219q
Other DBs
ExplorEnz - The Enzyme Database: 1.18.6.1
IUBMB Enzyme Nomenclature: 1.18.6.1
ExPASy - ENZYME nomenclature database: 1.18.6.1
UM-BBD (Biocatalysis/Biodegradation Database): 1.18.6.1
BRENDA, the Enzyme Database: 1.18.6.1
CAS: 9013-04-1

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