KEGG   ENZYME: 1.2.3.15Help
Entry
EC 1.2.3.15                 Enzyme                                 

Name
(methyl)glyoxal oxidase;
glx1 (gene name);
glx2 (gene name)
Class
Oxidoreductases;
Acting on the aldehyde or oxo group of donors;
With oxygen as acceptor
BRITE hierarchy
Sysname
(methyl)glyoxal:oxygen oxidoreductase
Reaction(IUBMB)
(1) glyoxal + H2O + O2 = glyoxylate + H2O2 [RN:R11351];
(2) 2-oxopropanal + H2O + O2 = pyruvate + H2O2 [RN:R11352]
Reaction(KEGG)
Substrate
glyoxal [CPD:C14448];
H2O [CPD:C00001];
O2 [CPD:C00007];
2-oxopropanal [CPD:C00546]
Product
glyoxylate [CPD:C00048];
H2O2 [CPD:C00027];
pyruvate [CPD:C00022]
Comment
The enzyme, originally characterized from the white rot fungus Phanerochaete chrysosporium, utilizes a free radical-coupled copper complex for catalysis.
History
EC 1.2.3.15 created 2016
Orthology
K20929  glyoxal/methylglyoxal oxidase
Reference
1  [PMID:3553159]
  Authors
Kersten PJ, Kirk TK
  Title
Involvement of a new enzyme, glyoxal oxidase, in extracellular H2O2 production by Phanerochaete chrysosporium.
  Journal
J Bacteriol 169:2195-201 (1987)
DOI:10.1128/JB.169.5.2195-2201.1987
Reference
2  [PMID:8346264]
  Authors
Kersten PJ, Cullen D
  Title
Cloning and characterization of cDNA encoding glyoxal oxidase, a H2O2-producing enzyme from the lignin-degrading basidiomycete Phanerochaete chrysosporium.
  Journal
Proc Natl Acad Sci U S A 90:7411-3 (1993)
DOI:10.1073/pnas.90.15.7411
  Sequence
Reference
3  [PMID:7592374]
  Authors
Kersten PJ, Witek C, vanden Wymelenberg A, Cullen D
  Title
Phanerochaete chrysosporium glyoxal oxidase is encoded by two allelic variants: structure, genomic organization, and heterologous expression of glx1 and glx2.
  Journal
J Bacteriol 177:6106-10 (1995)
DOI:10.1128/JB.177.21.6106-6110.1995
Reference
4  [PMID:8557673]
  Authors
Whittaker MM, Kersten PJ, Nakamura N, Sanders-Loehr J, Schweizer ES, Whittaker JW
  Title
Glyoxal oxidase from Phanerochaete chrysosporium is a new radical-copper oxidase.
  Journal
J Biol Chem 271:681-7 (1996)
DOI:10.1074/jbc.271.2.681
Other DBs
ExplorEnz - The Enzyme Database: 1.2.3.15
IUBMB Enzyme Nomenclature: 1.2.3.15
ExPASy - ENZYME nomenclature database: 1.2.3.15
BRENDA, the Enzyme Database: 1.2.3.15

DBGET integrated database retrieval system