KEGG   ENZYME: 1.2.7.4
Entry
EC 1.2.7.4                  Enzyme                                 
Name
anaerobic carbon monoxide dehydrogenase;
Ni-CODH;
carbon-monoxide dehydrogenase (ferredoxin)
Class
Oxidoreductases;
Acting on the aldehyde or oxo group of donors;
With an iron-sulfur protein as acceptor
Sysname
carbon-monoxide,water:ferredoxin oxidoreductase
Reaction(IUBMB)
CO + H2O + 2 oxidized ferredoxin = CO2 + 2 reduced ferredoxin + 2 H+ [RN:R07157]
Reaction(KEGG)
R07157;
(other) R08034 R09317
Substrate
CO [CPD:C00237];
H2O [CPD:C00001];
oxidized ferredoxin [CPD:C00139]
Product
CO2 [CPD:C00011];
reduced ferredoxin [CPD:C00138];
H+ [CPD:C00080]
Comment
This prokaryotic enzyme catalyses the reversible reduction of CO2 to CO. The electrons are transferred to redox proteins such as ferredoxin. In purple sulfur bacteria and methanogenic archaea it catalyses the oxidation of CO to CO2, which is incorporated by the Calvin-Benson-Basham cycle or released, respectively. In acetogenic and sulfate-reducing microbes it catalyses the reduction of CO2 to CO, which is incorporated into acetyl CoA by EC 2.3.1.169, CO-methylating acetyl-CoA synthase, with which the enzyme forms a tight complex in those organisms. The enzyme contains five metal clusters per homodimeric enzyme: two nickel-iron-sulfur clusters called the C-Clusters, one [4Fe-4S] D-cluster; and two [4Fe-4S] B-clusters. In methanogenic archaea additional [4Fe-4S] clusters exist, presumably as part of the electron transfer chain. In purple sulfur bacteria the enzyme forms complexes with the Ni-Fe-S protein EC 1.12.7.2, ferredoxin hydrogenase, which catalyse the overall reaction: CO + H2O = CO2 + H2. cf. EC 1.2.5.3, aerobic carbon monoxide dehydrogenase.
History
EC 1.2.7.4 created 2003 (EC 1.2.99.2 created 1982, modified 1990, modified 2003, incorporated 2015), modified 2016
Pathway
ec00633  Nitrotoluene degradation
ec00680  Methane metabolism
ec00720  Carbon fixation pathways in prokaryotes
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K00192  anaerobic carbon-monoxide dehydrogenase, CODH/ACS complex subunit alpha
K00198  anaerobic carbon-monoxide dehydrogenase catalytic subunit
Genes
CAMAF384_12620
AVNAvin_04490(cooS)
AVLAvCA_04490(cooS)
AVDAvCA6_04490(cooS)
ACXAchr_4450(cooS)
TMBThimo_0223
CCVCCV52592_1131(codH)
CCOCCC13826_0236(codH)
CGRACGRAC_1999
SULTFA592_03290(cooS)
GSUGSU2098(cooS)
GMEGmet_1902(cooS)
GPIGPICK_01000 GPICK_11385
GBZJZM60_09735(cooS) JZM60_15465(cooS)
GURGura_0618
GEOGeob_0362(cooS-1) Geob_1046(cooS-2)
GBMGbem_0072(cooS-2) Gbem_1736(cooS-1)
GSBGSUB_09760
GERKP004_20870(cooS)
GSUBKP001_15480(cooS)
GNTKP003_20810(cooS)
PCAPcar_0057(cooS-2) Pcar_0888(cooS-1)
PEFA7E78_09750
DESDSOUD_1182(cooS)
DEUDBW_0138
DVEDESUT3_02300
DVUDVU_2098(cooS)
DVLDvul_1133
DVMDvMF_2233
DVGDeval_1949
DDSDdes_0382
DEFCNY67_12835(cooS)
DFLDFE_2686
DSDGD606_18530(cooS)
DMADMR_25780
DCBC3Y92_11905(cooS)
DDEDde_3028
DMSE8L03_07465(cooS)
DSADesal_2508
DAFDesaf_2842
DASDaes_0529
DPIBN4_11760(cooS) BN4_20190(cooS)
DEJAWY79_01565 AWY79_06530 AWY79_06540
PPRFDPRO_1606(cooS) DPRO_3530
DDNDND132_2520
DSXGD604_08985(cooS)
PBIZLWC08_02100(cooS) LWC08_04395(cooS)
DBADbac_0934
DRTDret_1234
DSFUWK_01293 UWK_03164
DAKDaAHT2_0337 DaAHT2_0827
DBKDGMP_17830(cooS_1) DGMP_17900(cooS_2)
DOLDole_1461 Dole_3185
DMLDmul_09300(cooS1) Dmul_19100(cooS2) Dmul_30840(cdhA)
DALDalk_0680 Dalk_2379
DATHRM2_16670(cdhA) HRM2_41010(cdh1) HRM2_43440(cdh2)
DTOTOL2_C00990(cdh1) TOL2_C11820(cdhA) TOL2_C38110(cooS)
DOVDSCO28_32360(cooS_1) DSCO28_36040(cooS_2) DSCO28_44770 DSCO28_59740(cooS_3)
DWDDSCW_28520(cooS_1) DSCW_41580(cooS_2) DSCW_47510(cooS_3)
DALKDSCA_26710(cooS_1) DSCA_27610(cooS_2) DSCA_28100(cooS_3)
DLIdnl_13210(cdhA) dnl_20660(cooS)
DMMdnm_013300(cdhA1) dnm_058880(cooS) dnm_066390(cdhA2)
DEKDSLASN_16750(cooS_1) DSLASN_29000(cooS_2) DSLASN_47710(cooS_3)
DAODesac_0166 Desac_1966 Desac_2050
DTIDesti_0232
SFUSfum_2566 Sfum_2875
DAXFDQ92_02155(cooS) FDQ92_06245(cooS)
DBRDeba_0241
DHRLGS26_01950(cooS)
RPCRPC_4498
RRURru_A1427
RRFF11_07370
GMCGY4MC1_1807
GTHGeoth_1875
PTLAOT13_13405
BBEVBBEV_2326(cooS1)
PDUPDUR_14040
PSOPKP014_17220(cooS)
CACCA_C0116 CA_C2498
CAESMB_G0117(cooS) SMB_G2533
CAYCEA_G0117 CEA_G2512
CTCCTC_01156
CTETBN906_01229
CNONT01CX_0669(cooS)
CBOCBO1331 CBO2218
CBACLB_1359 CLB_2157
CBHCLC_1369
CBYCLM_1506 CLM_2421
CBLCLK_0775 CLK_1658
CBBCLD_2358 CLD_3226
CBICLJ_B1446 CLJ_B2428
CBNCbC4_2402(cooS)
CBFCLI_1426 CLI_2267
CBMCBF_1402 CBF_2255
CBJH04402_01401 H04402_02230
CBECbei_3020 Cbei_5054
CBZCbs_3020 Cbs_5054
CBEILF65_03401 LF65_05731
CKLCKL_2148
CKRCKR_1887
CLJCLJU_c09110(cooS1) CLJU_c17910(cooS2) CLJU_c37670(codH)
CCBClocel_0365 Clocel_1516 Clocel_2587 Clocel_4231
CLBClo1100_1430 Clo1100_3687
CSRCspa_c16740(cooS1) Cspa_c21120(cooS2) Cspa_c57670(cooS3)
CPASClopa_0057 Clopa_1484 Clopa_2128 Clopa_3232
CPATCLPA_c05080(cooS) CLPA_c34390(cooS1)
CPAECPAST_c05080(cooS) CPAST_c34390(cooS1)
CSBCLSA_c11710(cooS1) CLSA_c14830(cooS2)
CAHCAETHG_1621 CAETHG_3005 CAETHG_3899
CLTCM240_0400
CSQCSCA_1153 CSCA_1513 CSCA_3645 CSCA_4539
CLDCLSPO_c13520(cooS1) CLSPO_c22620(cooS2)
CACECACET_c09110(acsA1) CACET_c10200(acsA2) CACET_c16200 CACET_c26140(acsA3)
CCKCcar_07140 Ccar_08590 Ccar_18845 Ccar_23090
CTYKCTK_C21760(cooS)
CEUA7L45_14975
CFMBJL90_04040 BJL90_08730 BJL90_10555 BJL90_11075 BJL90_13810 BJL90_18885
CARGRSJ17_07130 RSJ17_07755
CDRKB9W14_01390 B9W14_11700 B9W14_13030 B9W14_22310
CDYF3K33_15405(cooS) F3K33_25905(cooS)
CCOHSAMEA4530647_0946(cooS1_1)
CLOSDMR38_04285(cooS) DMR38_08960(cooS) DMR38_18780(cooS) DMR38_21405(cooS)
CMAHC1I91_10775(cooS) C1I91_16460(cooS)
AMTAmet_1177 Amet_2016 Amet_3764
GFEGferi_05255 Gferi_06375 Gferi_11835 Gferi_24855
HHWNCTC503_01567(cooS1)
SARJHH195_01185(cooS)
PBIFKXZ80_13960(cooS) KXZ80_14960(cooS)
CAZOG3A45_00240(cooS)
CTHCthe_2801
CTXClo1313_0390
CCLClocl_0520 Clocl_0806
CCECcel_0438 Ccel_2918
RHEREHE19_006575(cooS)
CSSCst_c19650(cooS1)
CSDClst_1883
CTHDCDO33_08275(cooS)
RALRumal_3736
RUMCK1_12690
ROBCK5_28700
BYLA4V09_05450 A4V09_05810
BHANCGC63_06525(cooS) CGC63_07980(cooS)
BLAUDQQ01_10205(cooS)
BPROPMF13cell1_00639(cooS1_1) PMF13cell1_04203(cooS) PMF13cell1_04285(cooS1_2)
BLABEYS05_16735(cooS)
BLIQINP51_05945(cooS)
LACYA4V08_01520
CSHClosa_0921
EHLEHLA_2243
ACELacsn021_07870
RGNRGna_12250(cooS)
CBOLCGC65_03955(cooS) CGC65_24000(cooS)
CDFCD630_01740(cooS) CD630_07160(cooS1)
PDCCDIF630_00296 CDIF630_00832(cooS)
CDCCD196_0188 CD196_0661(cooS)
CDLCDR20291_0175 CDR20291_0643(cooS)
PDFCD630DERM_01740(cooS) CD630DERM_07160(cooS1)
CSTCLOST_1160
PSORRSJ16_15770
ROCHF520_11320(cooS)
PHXKGNDJEFE_00543(cooS)
TEMJW646_03340(cooS) JW646_10835(cooS) JW646_19155(cooS)
TEBT8CH_0649(cooS) T8CH_1211(cooS) T8CH_3032(cooS)
CALKHUE98_10145(cooS) HUE98_15625(cooS) HUE98_15635(cooS)
DSYDSY1653 DSY2630 DSY4173 DSY4442
DHDDhaf_0882 Dhaf_1166 Dhaf_2798 Dhaf_3792
DDHDesde_1134 Desde_2256 Desde_3264
DDLDesdi_0655 Desdi_0934 Desdi_1922
DMTDESME_03525 DESME_07325
SGYSgly_2814
DORDesor_0421 Desor_3274 Desor_4181 Desor_4446
DAIDesaci_0260 Desaci_0709 Desaci_2975
DMIDesmer_0456 Desmer_1790 Desmer_2536 Desmer_3209 Desmer_3478
DEDDHBDCA_p230(cooS1)
DECDCF50_p290(cooS1)
DRSDEHRE_00735
DCADesca_0349 Desca_1148 Desca_1990
DRMDred_0652
DRUDesru_0859 Desru_3320
DFGB0537_03940
DAEDtox_1270 Dtox_2327 Dtox_2933 Dtox_3780
DKUDesku_1492 Desku_2150 Desku_2787
DAUDaud_0105 Daud_0870
TFRBR63_06565(cooS)
FWADCMF_02985 DCMF_05870 DCMF_16840
AACXDEACI_0147 DEACI_1162 DEACI_2511
TJRTherJR_1157 TherJR_1937 TherJR_2144 TherJR_2959
DGIDesgi_0522 Desgi_1185 Desgi_2052 Desgi_2753 Desgi_3080
HCVFTV88_2859 FTV88_3326
ELMELI_3603
EMTCPZ25_018705(cooS)
ELIMB2M23_08400
AWOAwo_c10740(acsA) Awo_c19050(cooS)
ALKAJ0B03_11230(cooS)
CTHMCFE_0163 CFE_0173 CFE_0653 CFE_1617 CFE_1666 CFE_1919
ACAEHYG86_07690(cooS) HYG86_14090(cooS)
AMIJEQM06_02755(cooS)
AMICAmi3637_03990(cooS) Ami3637_14435
ABUTAmi103574_08595(cooS) Ami103574_09485
TTETTE1708
TKITKV_c08080(cooS) TKV_c20100(acsA)
CHYCHY_0034(cooSV) CHY_0085(cooSII) CHY_0736(cooSIV) CHY_1824(cooSI)
MTAMoth_1203 Moth_1972
MTHOMOTHE_c11790 MOTHE_c20130(cooS1)
MTHZMOTHA_c12660 MOTHA_c20910(cooS1)
ADGAdeg_0335
TPZTph_c05730(cooS1) Tph_c15180(acsB)
CSCCsac_0400
CHDCalhy_0239
CKICalkr_0175
CLCCalla_2155
TNRThena_0036
TACITDSAC_0035
TEPTepRe1_0548
TAETepiRe1_0598(cooS)
TOCToce_0335 Toce_0761 Toce_0800
NTHNther_0860
NCDACONDI_00123(cooS2) ACONDI_01147(cooS1) ACONDI_02970
HALSD7D81_07270(cooS) D7D81_07945(cooS)
IFNGM661_04805(cooS) GM661_05400(cooS)
AARAcear_0983 Acear_1305
HHLHalha_1234
CADCuri_c06440(cooS)
VPRVpar_0209
VATB7L28_00930
VRM44547418_00230(cooS2)
VDNNCTC11831_00196(cooS1)
VNKVEIT17_01990
MMAXLCQ47_06945(cooS)
SSGSelsp_2016
PUFUFO1_0648 UFO1_2774
PFTJBW_00105 JBW_04289
MANAMAMMFC1_00477 MAMMFC1_00822(cooS) MAMMFC1_02734(cooS2)
STEDSPTER_01630 SPTER_04690(cooS2_1) SPTER_06840(cooS2_2) SPTER_13670
KSTKSMBR1_1326(cdh_acsA) KSMBR1_3765(acsA_2)
BROCIPI25_11085(cooS)
BPITBPIT_02260
JETL3J17_07160(cooS)
TAZTREAZ_1535(cooS_2) TREAZ_1650(cooS_1)
TPITREPR_3069(cooS)
CPORBED41_06285
TOHBCB71_06635(cooS)
CPCCpar_1193
CPHCpha266_0149
PROSCHL67_00350
PMXPERMA_0106(cooS)
TAMTheam_1337
DTEDester_0417 Dester_0833
DINSelin_2012
DTPJZK55_05490(cooS)
TIDThein_1422 Thein_2190
TOPTOPB45_0398
CTHITHC_1754
TAVG4V39_00810(cooS) G4V39_01690(cooS)
TMAIFVE67_00270(cooS)
MJAMJ_0153 MJ_0728
MFEMefer_0203
MVUMetvu_0628 Metvu_1187
MFSMFS40622_0120 MFS40622_1444
MIFMetin_1030
MJHJH146_0004 JH146_1155
MESGMLAUSG7_0241(cooS) MLAUSG7_1297(cdhA)
MESAMLASG1_0903(cdhA) MLASG1_1434(cooS)
MIGMetig_0288 Metig_0357 Metig_1381
MMPMMP0985(cdhA)
MMQMmarC5_0805
MMXMmarC6_1673
MMZMmarC7_0227
MMDGYY_05750
MMAKMMKA1_10980(cdhA) MMKA1_11410(cdhA)
MMAOMMOS7_10770(cdhA)
MMADMMJJ_00060
MAEMaeo_0475 Maeo_1174
MVNMevan_0311
MVOMvol_0200
MOKMetok_0747
METFCFE53_02830(cdhA)
MTHMTH_1708
MMGMTBMA_c02870(cdhA1) MTBMA_c14200(cdhA2) MTBMA_c14210(cdhA3)
METCMTCT_1561
MWOMWSIV6_0249(cdhA1) MWSIV6_0986(cdhA3)
METEtca_00999 tca_01655
METKFVF72_03125(cdhA) FVF72_06415 FVF72_06420
MTHMFZP57_01125(cdhA)
METJFZP68_03850
MAROMarbSA_08810
MELMetbo_0462
MEWMSWAN_1934
METHMBMB1_1565(cdhA)
MFCBRM9_0801(cdhA)
MFIDSM1535_0763(cdhA)
MCUBMCBB_1821(cdhA)
MSUBBK009_06810
METNBK008_02455
METTCIT01_07270(cdhA)
METOCIT02_10120(cdhA)
MEMEHYG87_06525 HYG87_07900(cdhA)
MFVMfer_0527
MKAMK0536 MK0717(cdhA_1) MK0719(cdhA_1)
AFUAF_1100 AF_1849 AF_2397
AFGAFULGI_00011970 AFULGI_00021030 AFULGI_00026580
AVEArcve_1121
ASTAsulf_01231 Asulf_02029
FPLFerp_0731 Ferp_2369 Ferp_2562
GACGACE_0127 GACE_0359
GAHGAH_01139 GAH_01577
TONTON_1018
TGATGAM_0824(cooS)
TBATERMP_01153
THATAM4_1067 TAM4_582
THSTES1_1211
TGYX802_05885
TBSA3L01_08235
THHCDI07_07345(cooS)
TSLA3L11_01585
TPRFA3L09_01360
TRLA3L10_07625
MBAMbar_A0204 Mbar_A0434 Mbar_A3525 Mbar_A3717
MBYMSBRM_0628 MSBRM_1052 MSBRM_2317 MSBRM_2755
MBWMSBRW_0567 MSBRW_0792 MSBRW_1019 MSBRW_1209
MBARMSBR2_0095 MSBR2_1359 MSBR2_1750 MSBR2_2713
MBAKMSBR3_0095 MSBR3_2715 MSBR3_2955
MACMA_1016(cdhA) MA_1309(cooS) MA_3282(cooS) MA_3860(cdhA) MA_4399(cdhA)
MMAMM_0121 MM_0684 MM_2089 MM_2301
MMAZMmTuc01_0129 MmTuc01_0700 MmTuc01_2347
MMJMSMAS_0630 MSMAS_1212 MSMAS_2399 MSMAS_2596
MMACMSMAC_0632 MSMAC_1824 MSMAC_2048 MSMAC_2550
MVCMSVAZ_0995 MSVAZ_2289 MSVAZ_2495 MSVAZ_3017
MEKMSKOL_0967 MSKOL_2294 MSKOL_2507 MSKOL_3020
MLSMSLAZ_0649 MSLAZ_0996 MSLAZ_1465
METMMSMTP_0687 MSMTP_0695 MSMTP_0809 MSMTP_1179 MSMTP_1309
MEFMSWH1_0796 MSWH1_2253
MEQMSWHS_0848 MSWHS_2820 MSWHS_3011
MSJMSSAC_0985 MSSAC_3454 MSSAC_3597 MSSAC_4086
MSZMSSIH_0886 MSSIH_2982 MSSIH_3137 MSSIH_3621
MSWMSSIT_0920 MSSIT_3039 MSSIT_3208 MSSIT_3674
MTHRMSTHT_0128 MSTHT_2340
MTHEMSTHC_0600 MSTHC_0940
MHORMSHOH_0739 MSHOH_2496 MSHOH_3023 MSHOH_3042 MSHOH_3260
MFZAOB57_003735(cdhA)
MBUMbur_0858
MMETMCMEM_0990
MELOJ7W08_03300(cdhA)
MMHMmah_1166
MHAZBHR79_07705
MEVMetev_0426
MZHMzhil_1137
MPYMpsy_0694 Mpsy_2895
MZIHWN40_01045(cooS) HWN40_08225(cdhA)
MHZMetho_0975 Metho_1412
MTPMthe_0292 Mthe_1216 Mthe_1341
MCJMCON_1332(cdhA) MCON_2865(cooS)
MHIMhar_0789 Mhar_1630 Mhar_2328
MHUMhun_0690 Mhun_1272
MRTJKHC33_06770(cooS) KHC33_09830(cdhA) KHC33_13060(cooS)
MBGBN140_2260(cooS)
MEMAMMAB1_2986(cooS)
MCHKMchiMG62_13740(cooS)
MPIMpet_0907
MBNMboo_0884 Mboo_1373
MFOMetfor_1721
MPLMpal_0842
RCILRC456(cdhA)
HDSHSR122_2762
HDFAArcSl_0878(cooS)
SAIMK0C01_08745(cooS)
MELUMTLP_07180
MEAMMU439_06845(cooS)
MEARMpt1_c02980
TCBTCARB_0870
KCRKcr_0762
BARCAOA65_0464(cdhA2) AOA65_1716(cdhA)
BARBAOA66_1428(cdhA2)
 » show all
Reference
1  [PMID:6687389]
  Authors
Ragsdale SW, Clark JE, Ljungdahl LG, Lundie LL, Drake HL.
  Title
Properties of purified carbon monoxide dehydrogenase from Clostridium thermoaceticum, a nickel, iron-sulfur protein.
  Journal
J Biol Chem 258:2364-9 (1983)
Reference
2  [PMID:6687458]
  Authors
Diekert G, Ritter M.
  Title
Purification of the nickel protein carbon monoxide dehydrogenase of Clostridium thermoaceticum.
  Journal
FEBS Lett 151:41-4 (1983)
DOI:10.1016/0014-5793(83)80338-X
Reference
3  [PMID:3029096]
  Authors
Bonam D, Ludden PW.
  Title
Purification and characterization of carbon monoxide dehydrogenase, a nickel, zinc, iron-sulfur protein, from Rhodospirillum rubrum.
  Journal
J Biol Chem 262:2980-7 (1987)
Reference
4  [PMID:11593006]
  Authors
Drennan CL, Heo J, Sintchak MD, Schreiter E, Ludden PW.
  Title
Life on carbon monoxide: X-ray structure of Rhodospirillum rubrum Ni-Fe-S carbon monoxide dehydrogenase.
  Journal
Proc Natl Acad Sci U S A 98:11973-8 (2001)
DOI:10.1073/pnas.211429998
  Sequence
[rru:Rru_A1427]
Reference
5  [PMID:11509720]
  Authors
Dobbek H, Svetlitchnyi V, Gremer L, Huber R, Meyer O.
  Title
Crystal structure of a carbon monoxide dehydrogenase reveals a [Ni-4Fe-5S] cluster.
  Journal
Science 293:1281-5 (2001)
DOI:10.1126/science.1061500
  Sequence
[chy:CHY_0085]
Reference
6  [PMID:12386327]
  Authors
Doukov TI, Iverson TM, Seravalli J, Ragsdale SW, Drennan CL
  Title
A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase.
  Journal
Science 298:567-72 (2002)
DOI:10.1126/science.1075843
  Sequence
[mta:Moth_1203]
Reference
7  [PMID:24521136]
  Authors
Can M, Armstrong FA, Ragsdale SW
  Title
Structure, function, and mechanism of the nickel metalloenzymes, CO dehydrogenase, and acetyl-CoA synthase.
  Journal
Chem Rev 114:4149-74 (2014)
DOI:10.1021/cr400461p
Other DBs
ExplorEnz - The Enzyme Database: 1.2.7.4
IUBMB Enzyme Nomenclature: 1.2.7.4
ExPASy - ENZYME nomenclature database: 1.2.7.4
BRENDA, the Enzyme Database: 1.2.7.4

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