KEGG   ENZYME: 1.21.4.2
Entry
EC 1.21.4.2                 Enzyme                                 
Name
glycine reductase
Class
Oxidoreductases;
Catalysing the reaction X-H + Y-H = X-Y;
With a disulfide as acceptor
Sysname
acetyl-phosphate ammonia:thioredoxin disulfide oxidoreductase (glycine-forming)
Reaction(IUBMB)
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin [RN:R07226]
Reaction(KEGG)
R07226
Substrate
acetyl phosphate [CPD:C00227];
NH3 [CPD:C00014];
thioredoxin disulfide [CPD:C00343];
H2O [CPD:C00001]
Product
glycine [CPD:C00037];
phosphate [CPD:C00009];
thioredoxin [CPD:C00342]
Comment
The reaction is observed only in the direction of glycine reduction. The enzyme from Eubacterium acidaminophilum consists of subunits A, B and C. Subunit B contains selenocysteine and a pyruvoyl group, and is responsible for glycine binding and ammonia release. Subunit A, which also contains selenocysteine, is reduced by thioredoxin, and is needed to convert the carboxymethyl group into a ketene equivalent, in turn used by subunit C to produce acetyl phosphate. Only subunit B distinguishes this enzyme from EC 1.21.4.3 (sarcosine reductase) and EC 1.21.4.4 (betaine reductase).
History
EC 1.21.4.2 created 2003
Orthology
K10670  glycine/sarcosine/betaine reductase complex component A
K10671  glycine reductase complex component B subunit alpha and beta
K10672  glycine reductase complex component B subunit gamma
K21576  glycine/sarcosine/betaine reductase complex component C subunit alpha
K21577  glycine/sarcosine/betaine reductase complex component C subunit beta
Genes
EFEEFER_3013 EFER_3014 EFER_3015 EFER_3016 EFER_3017 EFER_3018 EFER_3019
YREHEC60_09230 HEC60_09240 HEC60_09250
MTHIC7M52_00974(grdA_1) C7M52_00975(grdA_2) C7M52_00976(grdC) C7M52_00977(grdD) C7M52_04026(grdH_1)
PPRPBPRB1543(GRDB) PBPRB1544(GRDE) PBPRB1546(PLSX2) PBPRB1547(FABH4) PBPRB1548 PBPRB1549
PGBH744_1c1269 H744_1c1270 H744_1c1271 H744_1c1273 H744_1c1274 H744_1c1275 H744_1c1276
EEINX720_06235(grdB) NX720_06240 NX720_06245(grdD) NX720_06250(grdC) NX720_06255(grdA)
DDSDdes_0112 Ddes_0113 Ddes_0115 Ddes_0116 Ddes_0118
DFIAXF13_12700 AXF13_12705 AXF13_12715 AXF13_12720 AXF13_12730 AXF13_12735 AXF13_12740
DPGDESPIGER_2436 DESPIGER_2437 DESPIGER_2438 DESPIGER_2442 DESPIGER_2443 DESPIGER_2445
DEFCNY67_00615 CNY67_00620(grdB) CNY67_00630 CNY67_00635 CNY67_00645
DMSE8L03_00865 E8L03_00870 E8L03_00885 E8L03_00890 E8L03_00900
DEOCAY53_05570
SATSYN_00803 SYN_00806 SYN_03718
DTIDesti_4380 Desti_4381 Desti_4382 Desti_4385 Desti_4386
GSAFOC50_03350 FOC50_03355 FOC50_03360(grdB) FOC50_03365 FOC50_03370
GHANCTC10459_00125(grdE) NCTC10459_00126(grdA) NCTC10459_00127(grdA1) NCTC10459_00128(grdB) NCTC10459_00130(grdC) NCTC10459_00131(grdD)
LGALGAS_1432
LPWLpgJCM5343_14370
PIOPI20285_06715
LALILA20249_02035
LSALCA_1691
LGMLG542_07605
EGVEGCR1_16410 EGCR1_16415 EGCR1_16420
EAVEH197_03925 EH197_03930 EH197_03935 EH197_13825 EH197_13830 EH197_13835(grdB) EH197_13850 EH197_13855
ERAFJ9537_06205 J9537_06215 J9537_06220 J9537_06240 J9537_06245 J9537_19265 J9537_19270 J9537_19275
VAHG7081_06145 G7081_06150 G7081_06155 G7081_06170(grdB) G7081_06175 G7081_06180
VCPH9L18_11775 H9L18_11780 H9L18_11785 H9L18_11800(grdB) H9L18_11805 H9L18_11810
VHYG7082_08770 G7082_08775 G7082_08780(grdB) G7082_08795 G7082_08800 G7082_08805
DPMFNV33_08340 FNV33_08345(grdB) FNV33_08365 FNV33_08370 FNV33_08385
CBOCBO1256(grdE) CBO1264(grdD)
CBACLB_1284(grdE) CLB_1285(grdB) CLB_1290(grdA) CLB_1291(grdC) CLB_1292(grdD)
CBHCLC_1296(grdE) CLC_1297(grdB) CLC_1301(grdC) CLC_1302(grdD)
CBYCLM_1415(grdE) CLM_1416(grdB) CLM_1420(grdA) CLM_1421(grdA) CLM_1422(grdC) CLM_1423(grdD)
CBLCLK_0696(grdE) CLK_0697(grdB) CLK_0701(grdA) CLK_0702 CLK_0703(grdC) CLK_0704(grdD)
CBBCLD_3303(grdD) CLD_3304(grdC) CLD_3305(grdA) CLD_3306 CLD_3309(grdB) CLD_3311(grdE)
CBICLJ_B1300(grdE) CLJ_B1301 CLJ_B1306(grdC) CLJ_B1307(grdD)
CBFCLI_1342 CLI_1343(grdB) CLI_1346(grdA) CLI_1347(grdC) CLI_1348(grdD)
CBMCBF_1315 CBF_1319(grdA) CBF_1320(grdA) CBF_1321(grdC) CBF_1322(grdD)
CBJH04402_01329 H04402_01330 H04402_01331 H04402_01334 H04402_01335 H04402_01336 H04402_01337
CLJCLJU_c27290 CLJU_c27300 CLJU_c27310 CLJU_c27770 CLJU_c27780 CLJU_c27790
CSQCSCA_2010 CSCA_2011 CSCA_2012 CSCA_2013 CSCA_2016 CSCA_2027 CSCA_2028 CSCA_2029 CSCA_2030 CSCA_2034 CSCA_3832 CSCA_3833 CSCA_3834 CSCA_3835 CSCA_3836 CSCA_3837 CSCA_3838
CLDCLSPO_c12650(grdE) CLSPO_c12660(grdB) CLSPO_c12690(grdA) CLSPO_c12700(grdC) CLSPO_c12710(grdD)
CACECACET_c07270(grdA1) CACET_c07280(grdC1) CACET_c07290(grdD1) CACET_c12280(grdE) CACET_c12290(grdA2) CACET_c12300(grdB) CACET_c12310(grdC2) CACET_c12320(grdD2)
CCKCcar_04470 Ccar_04490 Ccar_04495 Ccar_04500 Ccar_04505 Ccar_04550 Ccar_04565 Ccar_04570 Ccar_04575 Ccar_04580 Ccar_22155 Ccar_22160 Ccar_22165 Ccar_22170 Ccar_22175 Ccar_22180 Ccar_22185
CFMBJL90_09785 BJL90_09790 BJL90_09795 BJL90_09800 BJL90_09815 BJL90_19740 BJL90_19745 BJL90_19750 BJL90_19755 BJL90_19760 BJL90_19765 BJL90_19770
CARGRSJ17_03000 RSJ17_15270 RSJ17_15275 RSJ17_15280 RSJ17_15285 RSJ17_15295
CDRKB9W14_00360 B9W14_00365 B9W14_00370 B9W14_00375 B9W14_00380 B9W14_09400 B9W14_09405 B9W14_09410 B9W14_09485 B9W14_09490 B9W14_09495
CFERD4Z93_12125 D4Z93_12130 D4Z93_12135(grdB) D4Z93_12140 D4Z93_12145
CCAAKQH81_04740 KQH81_04745 KQH81_04750(grdB) KQH81_04760 KQH81_09940(grdB)
AMTAmet_3589 Amet_3590 Amet_3591 Amet_3592 Amet_3593
AOEClos_0956 Clos_0957 Clos_0958 Clos_0959 Clos_0960
GFEGferi_06245 Gferi_06250 Gferi_06255 Gferi_19040 Gferi_24385 Gferi_24390 Gferi_24395 Gferi_24400 Gferi_24405 Gferi_24410 Gferi_24415
HHWNCTC503_00667(grdE_1) NCTC503_00668(grdA) NCTC503_00669(grdA1) NCTC503_00670(grdB_1) NCTC503_00672(grdB_2) NCTC503_00673(grdC) NCTC503_00674(grdD) NCTC503_02631(grdB_3) NCTC503_02633(grdB_4) NCTC503_02634(grdE_2)
CALEFDN13_13560 FDN13_13565 FDN13_13570(grdB) FDN13_13575 FDN13_13580
CRSFQB35_05395 FQB35_05400 FQB35_05405(grdB) FQB35_05410 FQB35_05415
CPRFK7H06_10605 K7H06_10610 K7H06_10615(grdB) K7H06_10620 K7H06_10625 K7H06_10870 K7H06_10875 K7H06_10880
CRASKVH43_09675 KVH43_09680 KVH43_09900 KVH43_09905 KVH43_09910(grdB) KVH43_09915 KVH43_09920
PBIFKXZ80_12170 KXZ80_12175 KXZ80_12180(grdB) KXZ80_12185 KXZ80_12190
VCOPMM50RIKEN_00140(grdE) MM50RIKEN_00150(grdB) MM50RIKEN_00180(grdA) MM50RIKEN_00200(grdD)
CCOMI6K69_02915 I6K69_02920 I6K69_02925 I6K69_02940(grdB) I6K69_02945
LACYA4V08_08170 A4V08_14480 A4V08_14485 A4V08_14490 A4V08_14505 A4V08_16410 A4V08_16415 A4V08_16420 A4V08_16445 A4V08_16450
CSCIHDCHBGLK_02276(grdB_1) HDCHBGLK_02277(grdE_1) HDCHBGLK_02783(grdE_2) HDCHBGLK_02784(grdB_2) HDCHBGLK_02787(grdA) HDCHBGLK_02788(grdA1) HDCHBGLK_02789(grdC) HDCHBGLK_02790(grdD)
CSOCLS_08860 CLS_08870 CLS_08880 CLS_08910 CLS_08920 CLS_08930 CLS_08940
CBOLCGC65_00205 CGC65_00210(grdB) CGC65_00225 CGC65_00230 CGC65_00235 CGC65_01950 CGC65_01955 CGC65_01960 CGC65_12385 CGC65_20780 CGC65_22535 CGC65_22540 CGC65_25255 CGC65_25260
CASPNQ535_21090(grdA)
DLOK5I24_05100(grdD) K5I24_05110(grdA) K5I24_05125(grdB) K5I24_05130
SINTK5I26_03875 K5I26_03880 K5I26_04760(grdH) K5I26_11305 K5I26_11310(grdB) K5I26_11325(grdA) K5I26_11335(grdD) K5I26_12500(grdF)
CDFCD630_23480(grdD) CD630_23490(grdC) CD630_23510(grdB) CD630_23520(grdA) CD630_23540(grdE)
PDCCDIF630_02587(grdD) CDIF630_02588(grdC) CDIF630_02589(grdB) CDIF630_02592(grdA) CDIF630_02594(grdE)
CDCCD196_2191(grdD) CD196_2192(grdC) CD196_2193(grdB) CD196_2195(grdE)
CDLCDR20291_2237(grdD) CDR20291_2238(grdC) CDR20291_2239(grdB) CDR20291_2240(grdA) CDR20291_2241(grdE)
PDFCD630DERM_23480(grdD) CD630DERM_23490(grdC) CD630DERM_23510(grdB) CD630DERM_23520(grdA) CD630DERM_23540(grdE)
EACEAL2_808p07350(grdA4) EAL2_c02950(grdC2) EAL2_c02960(grdD2) EAL2_c08730(grdE1) EAL2_c08740(grdA2) EAL2_c08750(grdB2) EAL2_c16950(grdD) EAL2_c16960(grdC) EAL2_c16970(grdA1) EAL2_c17000(grdB1) EAL2_c17010(grdA3) EAL2_c17020(grd2)
CSTCLOST_1110(grdE) CLOST_1112(grdA) CLOST_1113(grdB) CLOST_1115(grdC) CLOST_1116(grdD)
PSORRSJ16_09825 RSJ16_09830 RSJ16_11920 RSJ16_14075 RSJ16_14080 RSJ16_14085 RSJ16_14090 RSJ16_14100
PHXKGNDJEFE_00988(grdB_2) KGNDJEFE_00989(grdB_3) KGNDJEFE_01774(grdD) KGNDJEFE_01775(grdC) KGNDJEFE_01776(grdB_4) KGNDJEFE_01777(grdB_5) KGNDJEFE_01778(grdA1_1) KGNDJEFE_01779(grdA1_2) KGNDJEFE_01780(grdE_2)
TEMJW646_05530 JW646_05545(grdB) JW646_05550 JW646_05560 JW646_05565(grdB) JW646_05570
TGCL0P85_02805(grdA) L0P85_02815 L0P85_02820(grdB) L0P85_02835(grdA) L0P85_02845(grdD) L0P85_05755(grdH) L0P85_05775(grdA)
TEBT8CH_0582(grdE) T8CH_0584 T8CH_0585(grdB) T8CH_1619(grdF) T8CH_3298(grdD) T8CH_3300
PBQC3V37_00600 C3V37_00605 C3V37_00610(grdB) C3V37_00615 C3V37_00620
STHSTH2865 STH2867 STH2869 STH2870(grdE)
SALQSYNTR_1292 SYNTR_1293 SYNTR_1294 SYNTR_1295 SYNTR_1296
CALKHUE98_12930 HUE98_12940 HUE98_12965
DORDesor_1546 Desor_1547 Desor_1548 Desor_1550 Desor_2136 Desor_2137 Desor_2138 Desor_2760 Desor_2761 Desor_2762 Desor_2763 Desor_2765 Desor_2766 Desor_2767
FWADCMF_21725 DCMF_21730 DCMF_21735 DCMF_22805 DCMF_22810 DCMF_22815 DCMF_22820 DCMF_22825 DCMF_22840 DCMF_22845 DCMF_22850 DCMF_22855 DCMF_22860 DCMF_23380 DCMF_23385 DCMF_23390 DCMF_23405 DCMF_23410 DCMF_23415 DCMF_23675 DCMF_23680 DCMF_23705 DCMF_23710 DCMF_23715 DCMF_23720 DCMF_25625 DCMF_25630 DCMF_25635 DCMF_25640
ACAEHYG86_10740 HYG86_10745 HYG86_10750(grdB) HYG86_10755 HYG86_10760
FMSM1R53_04475(grdD) M1R53_04485(grdB) M1R53_04490(grdA) M1R53_04495
BPRMCL3_17600 CL3_17610 CL3_17620 CL3_17650 CL3_17660 CL3_17670
TTETTE1874(PlsX2) TTE1875(FabH4) TTE1879
CHYCHY_2391(grdE) CHY_2392(grdA) CHY_2393(grdB) CHY_2394(grdC) CHY_2395(grdD)
GEKkuro4_05800(grdE) kuro4_05810 kuro4_05820(grdB) kuro4_05840(grdD)
TOCToce_1615 Toce_1616 Toce_1617 Toce_1618 Toce_1619 Toce_1620 Toce_1621 Toce_1680
NTHNther_0472 Nther_0473 Nther_0474 Nther_0475 Nther_0476 Nther_1043
HPKHprae_0133 Hprae_0134 Hprae_0137 Hprae_0142 Hprae_0143 Hprae_0450 Hprae_0451 Hprae_0452 Hprae_0453 Hprae_0454 Hprae_0456 Hprae_0457
HALSD7D81_09085 D7D81_09120 D7D81_09125
AARAcear_0988 Acear_0989 Acear_1058 Acear_1059 Acear_1060 Acear_1063 Acear_1064 Acear_2268 Acear_2269 Acear_2275 Acear_2280
HHLHalha_2193 Halha_2194
AFTBBF96_05405 BBF96_05410 BBF96_05415 BBF96_05420 BBF96_05425 BBF96_05430 BBF96_05435
KMEH0A61_00414(grdD) H0A61_00415(grdC) H0A61_00416(grdA_1) H0A61_00417(grdA_2) H0A61_00872(grdB) H0A61_00873(grdA1_1) H0A61_00874(grdA1_2) H0A61_00875(grdE)
FMAFMG_0424 FMG_0425 FMG_0426 FMG_0427 FMG_0428 FMG_0429 FMG_0430 FMG_0435 FMG_0436 FMG_1470 FMG_1606 FMG_1607 FMG_1608
PMICNW74_03525 NW74_03530 NW74_03535 NW74_03540 NW74_03545 NW74_03550 NW74_03555 NW74_05200 NW74_05205 NW74_05210 NW74_06415
MHAPJFY71_03890 JFY71_03895 JFY71_03900(grdB) JFY71_03905 JFY71_03910 JFY71_09955
SPOAEQM13_03650 EQM13_13045 EQM13_13050 EQM13_13055(grdB) EQM13_13060 EQM13_13065
KPARJL105_02470 JL105_02700 JL105_02705 JL105_02710 JL105_02715 JL105_02720 JL105_02725 JL105_02730
CAZOG3A45_03290 G3A45_03295 G3A45_03300(grdB) G3A45_03305 G3A45_03310
SEDZJYG23_01800 JYG23_01805(grdB) JYG23_01820 JYG23_01825 JYG23_01830
ECOUM1R54_01315(grdD) M1R54_01325(grdB) M1R54_01330(grdA) M1R54_01335
ETTAZF37_06570
PAUTPdca_16360 Pdca_16370(grdB) Pdca_16380 Pdca_16400(grdA)
SHIShel_21500 Shel_21510 Shel_25180 Shel_25190 Shel_25200 Shel_25230 Shel_25240
TIOINP52_07360 INP52_07370 INP52_07385
PLONPla110_06720
TDETDE_0078(grdB-1) TDE_0239(grdD) TDE_0240(grdC) TDE_0745(grdA) TDE_2119(grdB-2) TDE_2120(grdE-2)
TPEDTPE_0061 TPE_0159 TPE_0348(grdC) TPE_0349(grdD) TPE_1229(grdA) TPE_1230 TPE_2132(grdB-2) TPE_2133 TPE_2134(grdE-2)
TPKJO40_01150 JO40_01155 JO40_01160 JO40_04350 JO40_04355 JO40_06225 JO40_06230 JO40_06615 JO40_06620
TRMJO41_00635 JO41_00640 JO41_03205 JO41_03210 JO41_06515 JO41_06520 JO41_06525
TRZGWP43_01245 GWP43_01250 GWP43_02750 GWP43_02755 GWP43_05680
TMEDDWB79_02445(grdB) DWB79_02450 DWB79_06310 DWB79_06315 DWB79_06745 DWB79_06750 DWB79_10750
BRMBmur_2722 Bmur_2723 Bmur_2724 Bmur_2725 Bmur_2726 Bmur_2727 Bmur_2728
BPOBP951000_1854(grdE) BP951000_1855(grdA) BP951000_1856(grdB) BP951000_1857(grdC) BP951000_1858(grdC)
BPJB2904_orf2052 B2904_orf667(grdE) B2904_orf668(grdD) B2904_orf669(grdA) B2904_orf670(grdB) B2904_orf671(grdB) B2904_orf672(grdC) B2904_orf673(grdC)
BPIPBPP43_05895 BPP43_05900
BPWWESB_0748(grdE) WESB_0749(grdE1) WESB_0750(grdA) WESB_0751(grdA1) WESB_0752(grdB) WESB_0753 WESB_0754
LEOAMK43_03510 AMK43_03525 AMK43_03550 AMK43_09725 AMK43_09730 AMK43_09735 AMK43_09740 AMK43_09745 AMK43_09750
LGOJCM16774_0828(grdE) JCM16774_0829 JCM16774_0830 JCM16774_0831(grdB) JCM16774_0832 JCM16774_0833(grdC) JCM16774_0834(grdD) JCM16774_0837 JCM16774_0838 JCM16774_0844
TAITaci_1134 Taci_1135 Taci_1136 Taci_1137 Taci_1138
ACOAmico_1391 Amico_1392 Amico_1393 Amico_1394 Amico_1395 Amico_1396 Amico_1397
TLITlie_1191 Tlie_1192 Tlie_1193 Tlie_1285 Tlie_1286 Tlie_1288 Tlie_1289 Tlie_1290 Tlie_1460 Tlie_1461
AMOAnamo_0397 Anamo_0398 Anamo_0399 Anamo_0400 Anamo_0401 Anamo_0402 Anamo_1047 Anamo_1048 Anamo_1239 Anamo_1240 Anamo_1616 Anamo_1617
SBRSY1_16370 SY1_16390 SY1_16400 SY1_16420 SY1_16570
CPORBED41_00185 BED41_00190 BED41_00195 BED41_11180 BED41_11185 BED41_11190 BED41_11205 BED41_11210 BED41_11300 BED41_11585 BED41_11590 BED41_11595 BED41_12425 BED41_12430 BED41_12485 BED41_12500 BED41_12505 BED41_12510 BED41_14830 BED41_14835 BED41_15520 BED41_15525 BED41_15530 BED41_15540
PPIOCE91St28_01850(grdB_1) CE91St28_01860(grdE_1) CE91St28_01870 CE91St28_02800 CE91St28_03830(grdB_2) CE91St28_03840(grdE_2) CE91St28_12770(grdE_3) CE91St28_16000 CE91St28_19890 CE91St28_19900 CE91St28_24290 CE91St28_24300(grdB_3)
CPOCOPRO5265_01705 COPRO5265_01710 COPRO5265_0221 COPRO5265_0222 COPRO5265_0223 COPRO5265_0227 COPRO5265_0228
BARCAOA65_0941 AOA65_0942 AOA65_0943 AOA65_0944(grdA)
 » show all
Reference
1  [PMID:10091582]
  Authors
Wagner M, Sonntag D, Grimm R, Pich A, Eckerskorn C, Sohling B, Andreesen JR
  Title
Substrate-specific selenoprotein B of glycine reductase from Eubacterium acidaminophilum. Biochemical and molecular analysis.
  Journal
Eur J Biochem 260:38-49 (1999)
DOI:10.1046/j.1432-1327.1999.00107.x
  Sequence
Reference
2  [PMID:11422384]
  Authors
Bednarski B, Andreesen JR, Pich A.
  Title
In vitro processing of the proproteins GrdE of protein B of glycine reductase and PrdA of D-proline reductase from Clostridium sticklandii: formation of a pyruvoyl group from a cysteine residue.
  Journal
Eur J Biochem 268:3538-44 (2001)
DOI:10.1046/j.1432-1327.2001.02257.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.21.4.2
IUBMB Enzyme Nomenclature: 1.21.4.2
ExPASy - ENZYME nomenclature database: 1.21.4.2
BRENDA, the Enzyme Database: 1.21.4.2
CAS: 39307-24-9

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