KEGG   ENZYME: 1.21.99.5Help
Entry
EC 1.21.99.5                Enzyme                                 

Name
tetrachloroethene reductive dehalogenase;
tetrachloroethene reductase
Class
Oxidoreductases;
Catalysing the reaction X-H + Y-H = X-Y;
With unknown physiological acceptors
BRITE hierarchy
Sysname
acceptor:trichloroethene oxidoreductase (chlorinating)
Reaction(IUBMB)
trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor [RN:R05753]
Reaction(KEGG)
Substrate
trichloroethene [CPD:C06790];
chloride [CPD:C00698];
acceptor [CPD:C00028]
Product
tetrachloroethene [CPD:C06789];
reduced acceptor [CPD:C00030]
Comment
This enzyme allows the common pollutant tetrachloroethene to support bacterial growth and is responsible for disposal of a number of chlorinated hydrocarbons. The reaction occurs in the reverse direction. The enzyme also reduces trichloroethene to dichloroethene. Although the physiological reductant is unknown, the supply of reductant in some organisms involves menaquinol, which is reduced by molecular hydrogen via the action of EC 1.12.5.1, hydrogen:quinone oxidoreductase. The enzyme contains a corrinoid and two iron-sulfur clusters. Methylviologen can act as electron donor in vitro.
History
EC 1.21.99.5 created 2001 as EC 1.97.1.8, transferred 2017 to EC 1.21.99.5
Pathway
ec00625  Chloroalkane and chloroalkene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K21647  tetrachloroethene reductive dehalogenase
Genes
SMUL: SMUL_1531(pceA)
SHAL: SHALO_1495
Taxonomy
Reference
1
  Authors
Holliger, C, Wohlfarth, G. and Diekert, G.
  Title
Reductive dechlorination in the energy metabolism of anaerobic bacteria.
  Journal
FEMS Microbiol Rev 22:383-398 (1998)
Reference
2
  Authors
Glod, G., Angst, W., Holliger, C. and Schwarzenbach, R.P.
  Title
Corrinoid-mediated reduction of tetrachloroethene, trichloroethene, and trichlorofluoroethene in homogeneous aqueous solution: Reaction kinetics and reaction mechanisms.
  Journal
Environ Sci Technol 31:253-260 (1997)
Reference
3  [PMID:8663199]
  Authors
Neumann A, Wohlfarth G, Diekert G.
  Title
Purification and characterization of tetrachloroethene reductive dehalogenase from Dehalospirillum multivorans.
  Journal
J Biol Chem 271:16515-9 (1996)
DOI:10.1074/jbc.271.28.16515
Reference
4  [PMID:9224702]
  Authors
Schumacher W, Holliger C, Zehnder AJ, Hagen WR.
  Title
Redox chemistry of cobalamin and iron-sulfur cofactors in the tetrachloroethene reductase of Dehalobacter restrictus.
  Journal
FEBS Lett 409:421-5 (1997)
DOI:10.1016/S0014-5793(97)00520-6
Reference
5  [PMID:8636034]
  Authors
Schumacher W, Holliger C.
  Title
The proton/electron ration of the menaquinone-dependent electron transport from dihydrogen to tetrachloroethene in "Dehalobacter restrictus".
  Journal
J Bacteriol 178:2328-33 (1996)
DOI:10.1128/JB.178.8.2328-2333.1996
Other DBs
ExplorEnz - The Enzyme Database: 1.21.99.5
IUBMB Enzyme Nomenclature: 1.21.99.5
ExPASy - ENZYME nomenclature database: 1.21.99.5
BRENDA, the Enzyme Database: 1.21.99.5
CAS: 163913-51-7

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