KEGG   ENZYME: 1.3.1.102Help
Entry
EC 1.3.1.102                Enzyme                                 

Name
2-alkenal reductase (NADP+);
NADPH-dependent alkenal/one oxidoreductase;
NADPH:2-alkenal alpha,beta-hydrogenase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
n-alkanal:NADP+ 2-oxidoreductase
Reaction(IUBMB)
an n-alkanal + NADP+ = an alk-2-enal + NADPH + H+ [RN:R07350]
Reaction(KEGG)
Substrate
n-alkanal [CPD:C15596];
NADP+ [CPD:C00006]
Product
alk-2-enal [CPD:C15597];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
Shows highest activity with 1-nitrocyclohexene but also has significant activity with 2-methylpentenal and trans-cinnamaldehyde [3]. Involved in the detoxication of alpha,beta-unsaturated aldehydes and ketones. Has very low activity with NAD as reductant (cf. EC 1.3.1.74, 2-alkenal reductase [NAD(P)+]).
History
EC 1.3.1.102 created 2013
Orthology
K19825  2-alkenal reductase (NADP+)
Genes
SLY: 101246540
SPEN: 107021842
SOT: 102599848
CANN: 107868137
NTA: 107825579 107830910(DBR)
NSY: 104231555
NTO: 104093506
INI: 109156911
SIND: 105167157
Taxonomy
Reference
1  [PMID:11117876]
  Authors
Hirata T, Tamura Y, Yokobatake N, Shimoda K, Ashida Y
  Title
A 38 kDa allylic alcohol dehydrogenase from the cultured cells of Nicotiana tabacum.
  Journal
Phytochemistry 55:297-303 (2000)
DOI:10.1016/S0031-9422(00)00326-5
  Sequence
Reference
2  [PMID:17945329]
  Authors
Matsushima A, Sato Y, Otsuka M, Watanabe T, Yamamoto H, Hirata T
  Title
An enone reductase from Nicotiana tabacum: cDNA cloning, expression in Escherichia coli, and reduction of enones with the recombinant proteins.
  Journal
Bioorg Chem 36:23-8 (2008)
DOI:10.1016/j.bioorg.2007.08.005
  Sequence
Reference
3  [PMID:27547488]
  Authors
Mansell DJ, Toogood HS, Waller J, Hughes JM, Levy CW, Gardiner JM, Scrutton NS
  Title
Biocatalytic Asymmetric Alkene Reduction: Crystal Structure and Characterization  of a Double Bond Reductase from Nicotiana tabacum.
  Journal
ACS Catal 3:370-379 (2013)
DOI:10.1021/cs300709m
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.3.1.102
IUBMB Enzyme Nomenclature: 1.3.1.102
ExPASy - ENZYME nomenclature database: 1.3.1.102
BRENDA, the Enzyme Database: 1.3.1.102

DBGET integrated database retrieval system