KEGG   ENZYME: 1.3.1.108
Entry
EC 1.3.1.108                Enzyme                                 

Name
caffeoyl-CoA reductase;
electron-bifurcating caffeoyl-CoA reductase;
caffeoyl-CoA reductase-Etf complex;
hydrocaffeoyl-CoA:NAD+,ferredoxin oxidoreductase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
3-(3,4-dihydroxyphenyl)propanoyl-CoA:NAD+,ferredoxin oxidoreductase
Reaction(IUBMB)
3-(3,4-dihydroxyphenyl)propanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin [iron-sulfur] cluster = (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin [iron-sulfur] cluster [RN:R11674]
Reaction(KEGG)
R11674
Substrate
3-(3,4-dihydroxyphenyl)propanoyl-CoA [CPD:C21175];
NAD+ [CPD:C00003];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138]
Product
(2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA [CPD:C00323];
NADH [CPD:C00004];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139]
Comment
The enzyme, characterized from the bacterium Acetobacterium woodii, contains two [4Fe-4S] clusters and FAD. The enzyme couples the endergonic ferredoxin reduction with NADH as reductant to the exergonic reduction of caffeoyl-CoA with the same reductant. It uses the mechanism of electron bifurcation to overcome the steep energy barrier in ferredoxin reduction. It also reduces 4-coumaroyl-CoA and feruloyl-CoA.
History
EC 1.3.1.108 created 2015
Orthology
K22430  caffeyl-CoA reductase-Etf complex subunit CarC
K22431  caffeyl-CoA reductase-Etf complex subunit CarD
K22432  caffeyl-CoA reductase-Etf complex subunit CarE
Genes
CRS: FQB35_12685
CLO: HMPREF0868_0589 HMPREF0868_0590 HMPREF0868_0591(etfA)
CAPR: EQM14_02005 EQM14_02010 EQM14_02015
CSH: Closa_3003 Closa_3004 Closa_3005
LBW: C3V36_10425 C3V36_10430 C3V36_10435
AWO: Awo_c15720(carC) Awo_c15730(carD) Awo_c15740(carE)
Reference
1  [PMID:23479729]
  Authors
Bertsch J, Parthasarathy A, Buckel W, Muller V
  Title
An electron-bifurcating caffeyl-CoA reductase.
  Journal
J Biol Chem 288:11304-11 (2013)
DOI:10.1074/jbc.M112.444919
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.3.1.108
IUBMB Enzyme Nomenclature: 1.3.1.108
ExPASy - ENZYME nomenclature database: 1.3.1.108
BRENDA, the Enzyme Database: 1.3.1.108

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