KEGG   ENZYME: 1.3.1.109Help
Entry
EC 1.3.1.109                Enzyme                                 

Name
butanoyl-CoA dehydrogenase (NAD+, ferredoxin);
bifurcating butyryl-CoA dehydrogenase;
butyryl-CoA dehydrogenase/Etf complex;
Etf-Bcd complex;
bifurcating butanoyl-CoA dehydrogenase;
butanoyl-CoA dehydrogenase/Etf complex
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
butanoyl-CoA:NAD+, ferredoxin oxidoreductase
Reaction(IUBMB)
butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin [iron-sulfur] cluster = (E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin [iron-sulfur] cluster [RN:R11675]
Reaction(KEGG)
Substrate
butanoyl-CoA [CPD:C00136];
NAD+ [CPD:C00003];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138]
Product
(E)-but-2-enoyl-CoA [CPD:C00877];
NADH [CPD:C00004];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139]
Comment
This flavin containg enzyme, isolated from the bacteria Acidaminococcus fermentans and butanoate-producing Clostridia species, couples the exergonic reduction of (E)-but-2-enoyl-CoA to butanoyl-CoA with NADH to the endergonic reduction of ferredoxin by NADH, using electron bifurcation to overcome the steep energy barrier in ferredoxin reduction.
History
EC 1.3.1.109 created 2015
Reference
1  [PMID:17993531]
  Authors
Li F, Hinderberger J, Seedorf H, Zhang J, Buckel W, Thauer RK
  Title
Coupled ferredoxin and crotonyl coenzyme A (CoA) reduction with NADH catalyzed by the butyryl-CoA dehydrogenase/Etf complex from Clostridium kluyveri.
  Journal
J Bacteriol 190:843-50 (2008)
DOI:10.1128/JB.01417-07
  Sequence
Reference
2  [PMID:23772070]
  Authors
Aboulnaga el-H, Pinkenburg O, Schiffels J, El-Refai A, Buckel W, Selmer T
  Title
Effect of an oxygen-tolerant bifurcating butyryl coenzyme A dehydrogenase/electron-transferring flavoprotein complex from Clostridium difficile on butyrate production in Escherichia coli.
  Journal
J Bacteriol 195:3704-13 (2013)
DOI:10.1128/JB.00321-13
  Sequence
Reference
3  [PMID:24379410]
  Authors
Chowdhury NP, Mowafy AM, Demmer JK, Upadhyay V, Koelzer S, Jayamani E, Kahnt J, Hornung M, Demmer U, Ermler U, Buckel W
  Title
Studies on the mechanism of electron bifurcation catalyzed by electron transferring flavoprotein (Etf) and butyryl-CoA dehydrogenase (Bcd) of Acidaminococcus fermentans.
  Journal
J Biol Chem 289:5145-57 (2014)
DOI:10.1074/jbc.M113.521013
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.3.1.109
IUBMB Enzyme Nomenclature: 1.3.1.109
ExPASy - ENZYME nomenclature database: 1.3.1.109
BRENDA, the Enzyme Database: 1.3.1.109

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