KEGG   ENZYME: 1.3.1.114Help
Entry
EC 1.3.1.114                Enzyme                                 

Name
3-dehydro-bile acid Delta4,6-reductase;
baiN (gene name)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
3-oxocholan-24-oyl-CoA Delta4,6-oxidoreductase
Reaction(IUBMB)
(1) 3-oxocholan-24-oyl-CoA + NAD+ = 3-oxochol-4-en-24-oyl-CoA + NADH + H+ [RN:R12105];
(2) 3-oxochol-4-en-24-oyl-CoA + NAD+ = 3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+ [RN:R12106];
(3) 12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+ = 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ [RN:R12107];
(4) 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+ = 12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+ [RN:R12108]
Reaction(KEGG)
Substrate
3-oxocholan-24-oyl-CoA [CPD:C21905];
NAD+ [CPD:C00003];
3-oxochol-4-en-24-oyl-CoA [CPD:C21906];
12alpha-hydroxy-3-oxocholan-24-oyl-CoA [CPD:C21907];
12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA [CPD:C21908]
Product
3-oxochol-4-en-24-oyl-CoA [CPD:C21906];
NADH [CPD:C00004];
H+ [CPD:C00080];
3-oxochol-4,6-dien-24-oyl-CoA;
12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA [CPD:C21908];
12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA
Comment
Contains flavin. The enzyme, characterized from the bacterium Clostridium scindens, participates in the bile acid 7alpha-dehydroxylation pathway. The enzyme catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration.
History
EC 1.3.1.114 created 2018
Pathway
ec00121  Secondary bile acid biosynthesis
Orthology
K07007  3-dehydro-bile acid Delta4,6-reductase
Reference
1  [PMID:29217478]
  Authors
Harris SC, Devendran S, Alves JMP, Mythen SM, Hylemon PB, Ridlon JM
  Title
Identification of a gene encoding a flavoprotein involved in bile acid metabolism by the human gut bacterium Clostridium scindens ATCC 35704.
  Journal
Biochim Biophys Acta 1863:276-283 (2018)
DOI:10.1016/j.bbalip.2017.12.001
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.3.1.114
IUBMB Enzyme Nomenclature: 1.3.1.114
ExPASy - ENZYME nomenclature database: 1.3.1.114
BRENDA, the Enzyme Database: 1.3.1.114

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