KEGG   ENZYME: 1.3.1.115Help
Entry
EC 1.3.1.115                Enzyme                                 

Name
3-oxocholoyl-CoA 4-desaturase;
baiCD (gene name);
3-oxo-choloyl-CoA dehydrogenase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
3-oxocholoyl-CoA Delta4-oxidoreductase
Reaction(IUBMB)
(1) 7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+ = 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ [RN:R12109];
(2) 7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+ = 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ [RN:R12110]
Reaction(KEGG)
Substrate
7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA [CPD:C21910];
NAD+ [CPD:C00003];
7alpha-hydroxy-3-oxochol-24-oyl-CoA [CPD:C21909]
Product
7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA [CPD:C21469];
NADH [CPD:C00004];
H+ [CPD:C00080];
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA [CPD:C21471]
Comment
Contains flavin. The enzyme, characterized from the bacterium Clostridium scindens, participates in the bile acid 7alpha-dehydroxylation pathway. The enzyme catalyses the stereo-specific oxidation of its substrates and has no activity with the 7beta anomers. cf. EC 1.3.1.116, 7beta-hydroxy-3-oxochol-24-oyl-CoA 4-desaturase.
History
EC 1.3.1.115 created 2018
Pathway
ec00121  Secondary bile acid biosynthesis
Orthology
K15870  3-oxocholoyl-CoA 4-desaturase
Reference
1  [PMID:18047844]
  Authors
Kang DJ, Ridlon JM, Moore DR 2nd, Barnes S, Hylemon PB
  Title
Clostridium scindens baiCD and baiH genes encode stereo-specific 7alpha/7beta-hydroxy-3-oxo-delta4-cholenoic acid oxidoreductases.
  Journal
Biochim Biophys Acta 1781:16-25 (2008)
DOI:10.1016/j.bbalip.2007.10.008
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.3.1.115
IUBMB Enzyme Nomenclature: 1.3.1.115
ExPASy - ENZYME nomenclature database: 1.3.1.115
BRENDA, the Enzyme Database: 1.3.1.115

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