KEGG   ENZYME: 1.3.1.116Help
Entry
EC 1.3.1.116                Enzyme                                 

Name
7beta-hydroxy-3-oxochol-24-oyl-CoA 4-desaturase;
baiH (gene name)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
7beta-hydroxy-3-oxochol-24-oyl-CoA Delta4-oxidoreductase
Reaction(IUBMB)
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ = 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ [RN:R12111]
Reaction(KEGG)
Substrate
7beta-hydroxy-3-oxochol-24-oyl-CoA [CPD:C21911];
NAD+ [CPD:C00003]
Product
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA [CPD:C21912];
NADH [CPD:C00004];
H+ [CPD:C00080]
Comment
Contains FAD and FMN. The enzyme, characterized from the bacterium Clostridium scindens, participates in the bile acid 7alpha-dehydroxylation pathway. The enzyme catalyses the stereo-specific oxidation of its substrate and has no activity with the 7alpha anomer. cf. EC 1.3.1.115, 3-oxocholoyl-CoA 4-desaturase.
History
EC 1.3.1.116 created 2018
Pathway
ec00121  Secondary bile acid biosynthesis
Orthology
K15873  7beta-hydroxy-3-oxochol-24-oyl-CoA 4-desaturase
Reference
1  [PMID:7599167]
  Authors
Baron SF, Hylemon PB
  Title
Expression of the bile acid-inducible NADH:flavin oxidoreductase gene of Eubacterium sp. VPI 12708 in Escherichia coli.
  Journal
Biochim Biophys Acta 1249:145-54 (1995)
DOI:10.1016/0167-4838(95)00034-R
Reference
2  [PMID:8491719]
  Authors
Franklund CV, Baron SF, Hylemon PB
  Title
Characterization of the baiH gene encoding a bile acid-inducible NADH:flavin oxidoreductase from Eubacterium sp. strain VPI 12708.
  Journal
J Bacteriol 175:3002-12 (1993)
DOI:10.1128/JB.175.10.3002-3012.1993
  Sequence
Reference
3  [PMID:18047844]
  Authors
Kang DJ, Ridlon JM, Moore DR 2nd, Barnes S, Hylemon PB
  Title
Clostridium scindens baiCD and baiH genes encode stereo-specific 7alpha/7beta-hydroxy-3-oxo-delta4-cholenoic acid oxidoreductases.
  Journal
Biochim Biophys Acta 1781:16-25 (2008)
DOI:10.1016/j.bbalip.2007.10.008
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.3.1.116
IUBMB Enzyme Nomenclature: 1.3.1.116
ExPASy - ENZYME nomenclature database: 1.3.1.116
BRENDA, the Enzyme Database: 1.3.1.116

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