KEGG   ENZYME: 1.3.1.118Help
Entry
EC 1.3.1.118                Enzyme                                 

Name
meromycolic acid enoyl-[acyl-carrier-protein] reductase;
inhA (gene name)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
meromycolyl-[acyl-carrier protein]:NAD+ oxidoreductase
Reaction(IUBMB)
a meromycolyl-[acyl-carrier protein] + NAD+ = a trans-Delta2-meromycolyl-[acyl-carrier protein] + NADH + H+ [RN:R12236]
Reaction(KEGG)
Substrate
meromycolyl-[acyl-carrier protein] [CPD:C22023];
NAD+ [CPD:C00003]
Product
trans-Delta2-meromycolyl-[acyl-carrier protein] [CPD:C22022];
NADH [CPD:C00004];
H+ [CPD:C00080]
Comment
InhA is a component of the fatty acid synthase (FAS) II system of Mycobacterium tuberculosis, catalysing an enoyl-[acyl-carrier-protein] reductase step. The enzyme acts on very long and unsaturated fatty acids that form the meromycolic component of mycolic acids. It extends FASI-derived C20 fatty acids to form C60 to C90 mycolic acids. The enzyme, which forms a homotetramer, is the target of the preferred antitubercular drug isoniazid.
History
EC 1.3.1.118 created 2018
Orthology
K11611  meromycolic acid enoyl-[acyl-carrier-protein] reductase
Genes
MTU: Rv1484(inhA)
MTV: RVBD_1484
MTC: MT1531(inhA)
MRA: MRA_1494(inhA)
MTF: TBFG_11513
MTB: TBMG_02497
MTK: TBSG_02508
MTZ: TBXG_002476
MTG: MRGA327_09305
MTI: MRGA423_09315
MTUR: CFBS_1581(inhA)
MTO: MTCTRI2_1522(inhA)
MTD: UDA_1484(inhA)
MTN: ERDMAN_1652(inhA)
MTUC: J113_10355
MTUE: J114_07980
MTUL: TBHG_01463
MTUT: HKBT1_1581(inhA)
MTUU: HKBT2_1588(inhA)
MTQ: HKBS1_1585(inhA)
MBO: BQ2027_MB1520(inhA)
MBB: BCG_1546(inhA)
MBT: JTY_1521(inhA)
MBM: BCGMEX_1518(inhA)
MBX: BCGT_1322
MAF: MAF_15070(inhA)
MMIC: RN08_1663
MCE: MCAN_15011(inhA)
MCQ: BN44_20039(inhA)
MCV: BN43_30597(inhA)
MCX: BN42_21409(inhA)
MCZ: BN45_30583(inhA)
MLE: ML1806(inhA)
MLB: MLBr01806(inhA)
MPA: MAP_1210(inhA)
MAO: MAP4_2643
MAVI: RC58_13110
MAVU: RE97_13135
MAV: MAV_3294
MIT: OCO_31450
MIA: OCU_31350
MID: MIP_04657
MYO: OEM_30640
MIR: OCQ_32080
MLP: MLM_1780
MUL: MUL_1492(inhA)
MMC: Mmcs_2457
MKM: Mkms_2502
MJL: Mjls_2494
MMI: MMAR_2290(inhA)
MMAE: MMARE11_22120(inhA)
MMM: W7S_15555
MLI: MULP_03318(inhA)
MHAD: B586_13170
MSHG: MSG_02157(inhA)
MVA: Mvan_2753
MGI: Mflv_3657
MPHL: MPHLCCUG_02862(inhA_1)
MVQ: MYVA_2652
MTHN: 4412656_01968(inhA)
MHAS: MHAS_02110(inhA_1)
MAB: MAB_2722c
MABB: MASS_2668
MCHE: BB28_13590
MSTE: MSTE_02663
MJD: JDM601_2231(inhA)
MTER: 4434518_02153(inhA)
ASD: AS9A_1922
NFA: NFA_34760(inhA)
NFR: ERS450000_00572(inhA)
NCY: NOCYR_2630(inhA)
NNO: NONO_c41270(inhA)
RER: RER_30750(fabI)
REY: O5Y_14095
ROP: ROP_70020(fabI)
REQ: REQ_22500
RHB: NY08_1066
RFA: A3L23_04315(inhA_2)
RHS: A3Q41_03952(inhA_2)
RHU: A3Q40_01680(inhA_1)
RRT: 4535765_02252(inhA)
GBR: Gbro_2408
GPO: GPOL_c23410(inhA)
GOR: KTR9_2326
GRU: GCWB2_13270(inhA1)
GOM: D7316_00938(inhA)
TPR: Tpau_2082
SRT: Srot_2521
 » show all
Taxonomy
Reference
1  [PMID:7599116]
  Authors
Quemard A, Sacchettini JC, Dessen A, Vilcheze C, Bittman R, Jacobs WR Jr, Blanchard JS
  Title
Enzymatic characterization of the target for isoniazid in Mycobacterium tuberculosis.
  Journal
Biochemistry 34:8235-41 (1995)
Reference
2  [PMID:10336454]
  Authors
Rozwarski DA, Vilcheze C, Sugantino M, Bittman R, Sacchettini JC
  Title
Crystal structure of the Mycobacterium tuberculosis enoyl-ACP reductase, InhA, in complex with NAD+ and a C16 fatty acyl substrate.
  Journal
J Biol Chem 274:15582-9 (1999)
DOI:10.1074/jbc.274.22.15582
  Sequence
[mtu:Rv1484]
Reference
3  [PMID:10708367]
  Authors
Marrakchi H, Laneelle G, Quemard A
  Title
InhA, a target of the antituberculous drug isoniazid, is involved in a mycobacterial fatty acid elongation system, FAS-II.
  Journal
Microbiology 146 ( Pt 2):289-96 (2000)
DOI:10.1099/00221287-146-2-289
Reference
4  [PMID:10869086]
  Authors
Vilcheze C, Morbidoni HR, Weisbrod TR, Iwamoto H, Kuo M, Sacchettini JC, Jacobs WR Jr
  Title
Inactivation of the inhA-encoded fatty acid synthase II (FASII) enoyl-acyl carrier protein reductase induces accumulation of the FASI end products and cell  lysis of Mycobacterium smegmatis.
  Journal
J Bacteriol 182:4059-67 (2000)
DOI:10.1128/JB.182.14.4059-4067.2000
Reference
5  [PMID:18552191]
  Authors
Gurvitz A, Hiltunen JK, Kastaniotis AJ
  Title
Function of heterologous Mycobacterium tuberculosis InhA, a type 2 fatty acid synthase enzyme involved in extending C20 fatty acids to C60-to-C90 mycolic acids, during de novo lipoic acid synthesis in Saccharomyces cerevisiae.
  Journal
Appl Environ Microbiol 74:5078-85 (2008)
DOI:10.1128/AEM.00655-08
  Sequence
[mtu:Rv1484]
Reference
6  [PMID:25891098]
  Authors
Chollet A, Mourey L, Lherbet C, Delbot A, Julien S, Baltas M, Bernadou J, Pratviel G, Maveyraud L, Bernardes-Genisson V
  Title
Crystal structure of the enoyl-ACP reductase of Mycobacterium tuberculosis (InhA) in the apo-form and in complex with the active metabolite of isoniazid pre-formed by a biomimetic approach.
  Journal
J Struct Biol 190:328-37 (2015)
DOI:10.1016/j.jsb.2015.04.008
  Sequence
[mtu:Rv1484]
Other DBs
ExplorEnz - The Enzyme Database: 1.3.1.118
IUBMB Enzyme Nomenclature: 1.3.1.118
ExPASy - ENZYME nomenclature database: 1.3.1.118
BRENDA, the Enzyme Database: 1.3.1.118

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