KEGG   ENZYME: 1.3.1.118
Entry
EC 1.3.1.118                Enzyme                                 

Name
meromycolic acid enoyl-[acyl-carrier-protein] reductase;
inhA (gene name)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
meromycolyl-[acyl-carrier protein]:NAD+ oxidoreductase
Reaction(IUBMB)
a meromycolyl-[acyl-carrier protein] + NAD+ = a trans-Delta2-meromycolyl-[acyl-carrier protein] + NADH + H+ [RN:R12236]
Reaction(KEGG)
R12236
Substrate
meromycolyl-[acyl-carrier protein] [CPD:C22023];
NAD+ [CPD:C00003]
Product
trans-Delta2-meromycolyl-[acyl-carrier protein] [CPD:C22022];
NADH [CPD:C00004];
H+ [CPD:C00080]
Comment
InhA is a component of the fatty acid synthase (FAS) II system of Mycobacterium tuberculosis, catalysing an enoyl-[acyl-carrier-protein] reductase step. The enzyme acts on very long and unsaturated fatty acids that form the meromycolic component of mycolic acids. It extends FASI-derived C20 fatty acids to form C60 to C90 mycolic acids. The enzyme, which forms a homotetramer, is the target of the preferred antitubercular drug isoniazid.
History
EC 1.3.1.118 created 2018
Orthology
K11611  meromycolic acid enoyl-[acyl-carrier-protein] reductase
Genes
MXA: MXAN_6034
MSD: MYSTI_06661
MYM: A176_000862
MFB: MFUL124B02_34910
CCX: COCOR_06568(inhA)
MFU: LILAB_16130
MMAS: MYMAC_005815
SUR: STAUR_6711
AGE: AA314_08712
AVM: JQX13_03355(fabI)
MTU: Rv1484(inhA)
MTV: RVBD_1484
MTC: MT1531(inhA)
MRA: MRA_1494(inhA)
MTUR: CFBS_1581(inhA)
MTO: MTCTRI2_1522(inhA)
MTD: UDA_1484(inhA)
MTN: ERDMAN_1652(inhA)
MTUC: J113_10355
MTUE: J114_07980
MTUL: TBHG_01463
MTUT: HKBT1_1581(inhA)
MTUU: HKBT2_1588(inhA)
MTQ: HKBS1_1585(inhA)
MBO: BQ2027_MB1520(inhA)
MBB: BCG_1546(inhA)
MBT: JTY_1521(inhA)
MBM: BCGMEX_1518(inhA)
MBX: BCGT_1322
MAF: MAF_15070(inhA)
MMIC: RN08_1663
MCE: MCAN_15011(inhA)
MCQ: BN44_20039(inhA)
MCV: BN43_30597(inhA)
MCX: BN42_21409(inhA)
MCZ: BN45_30583(inhA)
MLE: ML1806(inhA)
MLB: MLBr01806(inhA)
MPA: MAP_1210(inhA)
MAO: MAP4_2643
MAVI: RC58_13110
MAVU: RE97_13135
MAV: MAV_3294
MIT: OCO_31450
MIA: OCU_31350
MID: MIP_04657
MYO: OEM_30640
MIR: OCQ_32080
MLP: MLM_1780
MMAN: MMAN_07200(inhA)
MUL: MUL_1492(inhA)
MMC: Mmcs_2457
MKM: Mkms_2502
MJL: Mjls_2494
MMI: MMAR_2290(inhA)
MMAE: MMARE11_22120(inhA)
MMM: W7S_15555
MLI: MULP_03318(inhA)
MHAD: B586_13170
MSHG: MSG_02157(inhA)
MFJ: MFLOJ_11050(inhA)
MSTO: MSTO_12230(inhA)
MSIM: MSIM_01960(inhA)
MSAK: MSAS_02520(inhA)
MGRO: FZ046_18515(fabI)
MXE: MYXE_28430(inhA)
MNV: MNVI_37780(inhA)
MNM: MNVM_38210(inhA)
MGOR: H0P51_12410(fabI)
MCOO: MCOO_05340(inhA)
MBAI: MB901379_02118(inhA_2)
MSEO: MSEO_09350(inhA)
MPSE: MPSD_23210(inhA)
MSHO: MSHO_35340(inhA)
MHEK: JMUB5695_02782(inhA_1)
MGAU: MGALJ_12570(inhA)
MLJ: MLAC_13510(inhA)
MBRD: MBRA_42300
MSHJ: MSHI_17260(inhA)
MKR: MKOR_10080(inhA)
MDF: K0O62_13785(fabI)
MMAM: K3U93_10435(fabI)
MHOL: K3U96_14075(fabI)
MHER: K3U94_12550(fabI)
MSEN: K3U95_12005(fabI)
MPAE: K0O64_13420(fabI)
MVA: Mvan_2753
MGI: Mflv_3657
MPHL: MPHLCCUG_02862(inhA_1)
MVQ: MYVA_2652
MTHN: 4412656_01968(inhA)
MHAS: MHAS_02110(inhA_1)
MDU: MDUV_16010(inhA)
MCHT: MCHIJ_41020(inhA)
MDR: MDOR_17270(inhA)
MAUU: NCTC10437_02605(inhA)
MMAG: MMAD_26350(inhA)
MMOR: MMOR_37220(inhA)
MAIC: MAIC_24400(inhA)
MALV: MALV_10030(inhA)
MTY: MTOK_44700(inhA)
MPSC: MPSYJ_39370(inhA)
MARZ: MARA_13230(inhA)
MGAD: MGAD_11680(inhA)
MHEV: MHEL_48950(inhA)
MSAR: MSAR_43940(inhA)
MANY: MANY_10900(inhA)
MAUB: MAUB_03720(inhA)
MPOF: MPOR_30680(inhA)
MPHU: MPHO_09120(inhA)
MMUC: C1S78_013650(fabI)
MMAT: MMAGJ_61610(inhA)
MBOK: MBOE_44970(inhA)
MFG: K6L26_17720(fabI)
MAB: MAB_2722c
MABB: MASS_2668
MCHE: BB28_13590
MSTE: MSTE_02663
MSAL: DSM43276_02423(inhA_3)
MJD: JDM601_2231(inhA)
MTER: 4434518_02153(inhA)
MMIN: MMIN_38310(inhA)
MHIB: MHIB_16780(inhA)
ASD: AS9A_1922
NFA: NFA_34760(inhA)
NFR: ERS450000_00572(inhA)
NCY: NOCYR_2630(inhA)
NNO: NONO_c41270(inhA)
NOD: FOH10_14740(fabI)
NAH: F5544_27300(fabI)
NAD: NCTC11293_04846(inhA)
NIE: KV110_26815(fabI)
RER: RER_30750(fabI)
REY: O5Y_14095
ROP: ROP_70020(fabI)
REQ: REQ_22500
RHB: NY08_1066
RFA: A3L23_04315(inhA_2)
RHS: A3Q41_03952(inhA_2)
RHU: A3Q40_01680(inhA_1)
RRT: 4535765_02252(inhA)
RCR: NCTC10994_00569(inhA)
RTM: G4H71_21230(fabI)
RKO: JWS14_37785(fabI)
RGO: KYT97_03610(fabI)
GBR: Gbro_2408
GPO: GPOL_c23410(inhA)
GOR: KTR9_2326
GRU: GCWB2_13270(inhA1)
GOM: D7316_00938(inhA)
GOD: GKZ92_12030(fabI)
GAM: GII34_12130(fabI)
GPD: GII33_11425(fabI)
GJI: H1R19_11600(fabI)
SPIN: KV203_10195(fabI)
TPR: Tpau_2082
SRT: Srot_2521
TOY: FO059_08845(fabI)
SCO: SCO1814(inhA)
SALB: XNR_5008
SMA: SAVERM_2295(fabI2) SAVERM_6463(fabI1)
SGR: SGR_5682
SGB: WQO_06910
SHY: SHJG_3265
SMAL: SMALA_1764
SSOI: I1A49_11980(fabI)
SCT: SCAT_1015(inhA)
SFA: Sfla_5004
SBH: SBI_08182
SVE: SVEN_1449
SDV: BN159_6718(inhA)
STRP: F750_1673
SFI: SFUL_1387
SALU: DC74_2313
SALL: SAZ_12925
STRE: GZL_06698
SLD: T261_6340
STRM: M444_09445
SAMB: SAM23877_1882(inhA)
SPRI: SPRI_5710
SRW: TUE45_02288(inhA_1)
SLE: sle_53190(sle_53190)
SRN: A4G23_01043(inhA)
SKY: D0C37_27055(fabI)
SALW: CP975_07580(fabI) CP975_22890(fabI)
SNR: SNOUR_31185(inhA)
STRD: NI25_30140
SLAU: SLA_1364
SALF: SMD44_01946(fabI)
SALJ: SMD11_5183(fabI)
SLX: SLAV_28340(inhA2)
SGE: DWG14_06601(inhA_4)
SGD: ELQ87_00785(fabI) ELQ87_30520(fabI)
SQZ: FQU76_05780(fabI)
SCYA: EJ357_10365(fabI)
SNQ: CP978_08605(fabI)
SKA: CP970_06910(fabI) CP970_34065(fabI)
SVN: CP980_25715(fabI)
SNK: CP967_27260(fabI)
SRK: FGW37_07670(fabI)
SFIC: EIZ62_26025(fabI)
SGAL: CP966_07680(fabI)
SVR: CP971_08720(fabI)
SFY: GFH48_31120(fabI)
SAQU: EJC51_12120(fabI) EJC51_43830(fabI)
SSEO: D0Z67_05985(fabI)
SBY: H7H31_03555(fabI) H7H31_28205(fabI)
SRIM: CP984_17925(fabI) CP984_31900(fabI)
SSUB: CP968_25520(fabI)
SPHV: F9278_08945(fabI)
SCIN: CP977_08145(fabI)
SCHA: CP983_33715(fabI)
SGX: H4W23_09165(fabI)
SCOE: CP976_10275(fabI) CP976_32245(fabI)
SBAT: G4Z16_27450(fabI)
SSPB: CP982_02960(fabI) CP982_10720(fabI)
SNF: JYK04_02485(inhA_2)
SPLA: CP981_09050(fabI)
SBRO: GQF42_11855(fabI)
SCHF: IPT68_08275(fabI)
SHUN: DWB77_05910(inhA_2)
SHAR: HUT13_04585(fabI)
SCYG: S1361_09975(inhA)
SFEU: IM697_13265(fabI)
SLIA: HA039_26885(fabI)
SDW: K7C20_08555(fabI) K7C20_18315(fabI)
SVD: CP969_10045(fabI)
SCAL: I6J39_06245(fabI)
SAUH: SU9_007030(fabI)
SFB: CP974_05410(fabI)
SMOB: J7W19_06960(fabI)
SPRA: CP972_08110(fabI)
KSK: KSE_57090(fabI)
LXL: KDY119_01609(inhA)
PSIC: J4E96_07270(fabI)
AQG: HRU87_03895(fabI)
ACH: Achl_4547
BCV: Bcav_2321
JDE: Jden_1165
DAY: FV141_06860(fabI)
LMOI: VV02_14755
YIM: J5M86_07145(fabI)
XCE: Xcel_1417
XYL: ET495_03195(fabI)
IDO: I598_0858(inhA_2)
XYA: ET471_12825(fabI)
SANW: G7063_06995(fabI)
CFI: Celf_1715
CELZ: E5225_09815(fabI)
CEJ: GC089_10160(fabI)
CELH: GXP71_05365(fabI)
OEK: FFI11_012230(fabI)
PSEI: GCE65_06025(fabI)
JLI: EXU32_06225(fabI)
JME: EEW87_007195(fabI)
TEH: GKE56_04255(fabI)
PHW: G7075_06800(fabI)
PEI: H9L10_05680(fabI)
SERJ: SGUI_0279
SERW: FY030_08320(fabI)
ORZ: FNH13_11155(fabI)
BLIN: BLSMQ_2126
BRI: FDF13_11380(fabI)
BLUT: EW640_03225(fabI)
BCAU: I6G59_13050(fabI)
DCO: SAMEA4475696_1748(inhA)
AUS: IPK37_17625(fabI)
GEZ: FE251_06575(fabI)
HALT: IM660_08460(fabI)
PAC: PPA1532
PAW: PAZ_c16210(inhA)
PACC: PAC1_08060
PACH: PAGK_0647
CACN: RN83_08130
CGRN: 4412665_01083(inhA)
PFR: PFREUD_14860(inhA)
PFRE: RM25_1407
PACD: EGX94_10235(fabI)
PAUS: NCTC13651_01795(inhA)
ARUB: J5A65_09785(fabI)
ACIJ: JS278_01256(inhA)
MPH: MLP_14730(fabI)
MIK: FOE78_17410(fabI)
MICG: GJV80_03390(fabI)
TDF: H9L22_03480(fabI)
TLA: TLA_TLA_02697(inhA)
RAIN: Rai3103_16520(fabI)
PRV: G7070_02480(fabI)
BRT: J4N02_11750(fabI)
NCA: Noca_2572
NDK: I601_3386(inhA)
NOY: EXE57_01475(fabI)
NOI: FCL41_08230(fabI)
NOO: FE634_10005(fabI)
NSN: EXE58_17975(fabI)
NANO: G5V58_10490(fabI)
NMES: H9L09_21000(fabI)
NPI: G7071_15280(fabI)
NAQU: ENKNEFLB_02471(inhA)
NRO: K8W59_09210(fabI)
PSIM: KR76_12900
MGG: MPLG2_2007(inhA)
KFL: Kfla_3713
KQI: F1D05_37065(fabI)
TFU: Tfu_1842
NDA: Ndas_2777
NAL: B005_5318(inhA)
STRR: EKD16_14180(inhA)
TCU: Tcur_2278
ACTW: F7P10_00815(fabI)
SRO: Sros_5937
NOW: GBF35_32365(fabI)
NCX: Nocox_26320(inhA2)
TBI: Tbis_1940
FAL: FRAAL4522
ACE: Acel_1152
NML: Namu_2302
NAK: EH165_07680(fabI)
GOB: Gobs_2105
BSD: BLASA_3113(inhA)
MMAR: MODMU_2062(fabI)
SEN: SACE_3786(inhA)
SACG: FDZ84_31765(fabI)
SACC: EYD13_12430(inhA1)
AMD: AMED_4940
AMN: RAM_25150
AMM: AMES_4881
AMZ: B737_4881
AOI: AORI_3781(fabI)
AJA: AJAP_20255(inhA)
AMQ: AMETH_3039(inhA)
AMYY: YIM_27545(inhA4)
AORI: SD37_19395
PDX: Psed_3370
PSEA: WY02_02980
PSEE: FRP1_12610
PSEH: XF36_09270
PBRO: HOP40_24560(fabI)
AMI: Amir_2181
SESP: BN6_31210(inhA)
SSYI: EKG83_34400(fabI)
KAL: KALB_2997
ACTI: UA75_11620
ACAD: UA74_11535
AHG: AHOG_10870(inhA2)
ALO: CRK56220
SACE: GIY23_10485(fabI)
SAQ: Sare_3250
MIL: ML5_4063
MICH: FJK98_22710(fabI)
MTEM: GCE86_15535(fabI)
MCRA: ID554_25030(fabI)
ASE: ACPL_1918(inhA)
ACTN: L083_2247 L083_2329(inhA)
AFS: AFR_11045
ACTS: ACWT_1796
VER: HUT12_22305(fabI)
DVC: Dvina_10550(fabI)
CAI: Caci_2302
SNA: Snas_2497
NAV: JQS30_07095(fabI)
GLY: K3N28_15275(fabI)
AIR: NCTC12972_00999(inhA)
ASLA: NCTC11923_01677(inhA)
AVC: NCTC10951_00535(inhA)
EKE: EK0264_18680(fabI)
RXY: Rxyl_0853
RUB: GBA63_06600(fabI)
RMAR: GBA65_13960(fabI)
BSOL: FSW04_06040(fabI)
CWO: Cwoe_4156
AFO: Afer_1232
AYM: YM304_19680(fabI)
ATQ: GH723_08465(fabI)
AQZ: KSP35_01010(fabI)
IAM: HC251_06115(fabI)
ERZ: ER308_20395(fabI)
 » show all
Reference
1  [PMID:7599116]
  Authors
Quemard A, Sacchettini JC, Dessen A, Vilcheze C, Bittman R, Jacobs WR Jr, Blanchard JS
  Title
Enzymatic characterization of the target for isoniazid in Mycobacterium tuberculosis.
  Journal
Biochemistry 34:8235-41 (1995)
DOI:10.1021/bi00026a004
Reference
2  [PMID:10336454]
  Authors
Rozwarski DA, Vilcheze C, Sugantino M, Bittman R, Sacchettini JC
  Title
Crystal structure of the Mycobacterium tuberculosis enoyl-ACP reductase, InhA, in complex with NAD+ and a C16 fatty acyl substrate.
  Journal
J Biol Chem 274:15582-9 (1999)
DOI:10.1074/jbc.274.22.15582
  Sequence
[mtu:Rv1484]
Reference
3  [PMID:10708367]
  Authors
Marrakchi H, Laneelle G, Quemard A
  Title
InhA, a target of the antituberculous drug isoniazid, is involved in a mycobacterial fatty acid elongation system, FAS-II.
  Journal
Microbiology 146 ( Pt 2):289-96 (2000)
DOI:10.1099/00221287-146-2-289
Reference
4  [PMID:10869086]
  Authors
Vilcheze C, Morbidoni HR, Weisbrod TR, Iwamoto H, Kuo M, Sacchettini JC, Jacobs WR Jr
  Title
Inactivation of the inhA-encoded fatty acid synthase II (FASII) enoyl-acyl carrier protein reductase induces accumulation of the FASI end products and cell  lysis of Mycobacterium smegmatis.
  Journal
J Bacteriol 182:4059-67 (2000)
DOI:10.1128/JB.182.14.4059-4067.2000
Reference
5  [PMID:18552191]
  Authors
Gurvitz A, Hiltunen JK, Kastaniotis AJ
  Title
Function of heterologous Mycobacterium tuberculosis InhA, a type 2 fatty acid synthase enzyme involved in extending C20 fatty acids to C60-to-C90 mycolic acids, during de novo lipoic acid synthesis in Saccharomyces cerevisiae.
  Journal
Appl Environ Microbiol 74:5078-85 (2008)
DOI:10.1128/AEM.00655-08
  Sequence
[mtu:Rv1484]
Reference
6  [PMID:25891098]
  Authors
Chollet A, Mourey L, Lherbet C, Delbot A, Julien S, Baltas M, Bernadou J, Pratviel G, Maveyraud L, Bernardes-Genisson V
  Title
Crystal structure of the enoyl-ACP reductase of Mycobacterium tuberculosis (InhA) in the apo-form and in complex with the active metabolite of isoniazid pre-formed by a biomimetic approach.
  Journal
J Struct Biol 190:328-37 (2015)
DOI:10.1016/j.jsb.2015.04.008
  Sequence
[mtu:Rv1484]
Other DBs
ExplorEnz - The Enzyme Database: 1.3.1.118
IUBMB Enzyme Nomenclature: 1.3.1.118
ExPASy - ENZYME nomenclature database: 1.3.1.118
BRENDA, the Enzyme Database: 1.3.1.118

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