Entry
Name
2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing];
fadH (gene name);
4-enoyl-CoA reductase (NADPH) (ambiguous);
4-enoyl coenzyme A (reduced nicotinamide adenine dinucleotide phosphate) reductase (ambiguous);
4-enoyl-CoA reductase (ambiguous);
2,4-dienoyl-CoA reductase (NADPH) (ambiguous);
trans-2,3-didehydroacyl-CoA:NADP+ 4-oxidoreductase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
(2E)-2-enoyl-CoA:NADP+ 4-oxidoreductase
Reaction(IUBMB)
(1) a (2E)-2-enoyl-CoA + NADP+ = a (2E,4E)-2,4-dienoyl-CoA + NADPH + H+ [RN:
R04319 ];
(2) a (2E)-2-enoyl-CoA + NADP+ = a (2E,4Z)-2,4-dienoyl-CoA + NADPH + H+ [RN:
R12620 ]
Reaction(KEGG)
Substrate
Product
Comment
This bacterial enzyme catalyses the reduction of either (2E,4E)-2,4-dienoyl-CoA or (2E,4Z)-2,4-dienoyl-CoA to (2E)-2-enoyl-CoA. The enzyme from Escherichia coli contains FAD, FMN, and an [4Fe-4S] iron sulfur cluster. cf. EC
1.3.1.124 , 2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing].
History
EC 1.3.1.34 created 1984, modified 1986, modified 2020
Orthology
K00219 2,4-dienoyl-CoA reductase (NADPH2)
Genes
LMAT : 92512665(LSCM1_02571)
LOI : 92358361(LSCM4_02396)
ECOO : ECRM13514_4043(ygjL)
ECOH : ECRM13516_3846(ygjL)
ECW : EcE24377A_3548(fadH)
ECOA : APECO78_19210(fadH)
EDJ : ECDH1ME8569_2976(fadH)
ECOI : ECOPMV1_03399(fadH)
SETC : CFSAN001921_00910(fadH)
SEEN : SE451236_22225(fadH)
SENH : CFSAN002069_16040(fadH)
SEEH : SEEH1578_02235(fadH)
SENJ : CFSAN001992_17440(fadH)
SEEC : CFSAN002050_23085(fadH)
SEEB : SEEB0189_003770(fadH)
REE : electrica_00585(fadH)
RTG : NCTC13098_00808(fadH)
CLAP : NCTC11466_04023(fadH)
KIE : NCTC12125_04541(fadH)
AHN : NCTC12129_00667(fadH_1)
PSHI : SAMEA2665130_2108(fadH)
YPG : YpAngola_A1093(fadH)
YPI : YpsIP31758_0501(fadH)
SMW : SMWW4_v1c42730(fadH)
SFJ : SAMEA4384070_4274(fadH)
SOF : NCTC11214_02363(fadH)
PLF : PANA5342_0605(fadH1)
PHEI : NCTC12003_03186(fadH)
PRJ : NCTC6933_03278(fadH)
XFU : XFF4834R_chr10160(fadH)
XPH : XppCFBP6546_06435(XppCFBP6546P_06435)
XHD : LMG31886_33360(fadH)
LLZ : LYB30171_02032(fadH)
VAU : VANGNB10_cI0942(fadH)
VPL : SA104470976_01696(fadH)
PAE : PA3092(fadH1) PA4814(fadH2)
PAEV : N297_3198 N297_4982
PAEI : N296_3198 N296_4982
PAU : PA14_24170(fadH1) PA14_63640(fadH2)
PAP : PSPA7_2046(fadH1) PSPA7_5533(fadH2)
PAG : PLES_19681(fadH1) PLES_51991(fadH2)
PAF : PAM18_1871(fadH1) PAM18_4924(fadH2)
PNC : NCGM2_0716(fadH2) NCGM2_4211(fadH1)
PAEB : NCGM1900_3210(fadH1) NCGM1900_5551(fadH2)
PDK : PADK2_08875 PADK2_25550
PSG : G655_09335 G655_25395
PRP : M062_16515(fadH) M062_25415(fadH)
PAEP : PA1S_09920(fadH) PA1S_26170(fadH)
PAER : PA1R_gp0928 PA1R_gp2734
PAEM : U769_09460(fadH) U769_26425(fadH)
PAEL : T223_09940(fadH) T223_26620
PAES : SCV20265_1956 SCV20265_5471
PAEU : BN889_03453(fadH1) BN889_05359(fadH2_2)
PAEG : AI22_07835 AI22_23940(fadH)
PAEC : M802_3196 M802_4980
PAEO : M801_3063 M801_4847
SECH : B18_11805 B18_28435
PCQ : PcP3B5_17940(fadH_1) PcP3B5_55680(fadH_3)
PMUI : G4G71_06560 G4G71_20650
PNT : G5B91_22405 G5B91_28815
PPAA : B7D75_09605(fadH) B7D75_25050(fadH)
PSAV : PSA3335_20385(fadH)
PVD : CFBP1590__3783(fadH)
PPRC : PFLCHA0_c40280(fadH)
PMUD : NCTC8068_03144(fadH)
PFW : PF1751_v1c33530(fadH)
PFF : PFLUOLIPICF719450(fadH)
PPUU : PputUW4_01742(fadH)
PDR : H681_16625 H681_21555
PKC : PKB_3852(fadh1) PKB_5141(fadh3)
POJ : PtoMrB4_07770(fadH2) PtoMrB4_37970(fadH1)
PTW : TUM18999_17320(fadH1) TUM18999_53870(fadH2)
PTAE : NCTC10697_02577(fadH)
PSOA : PSm6_13470(fadH2) PSm6_38060(fadH1)
PTRL : OU419_19995 OU419_24775
PDEN : F1C79_14030 F1C79_20345
MHC : MARHY1221(fadH) MARHY2740(fadH)
MAD : HP15_1058(fadH) HP15_2580(fadH)
MARI : ACP86_03225(fadH) ACP86_16255(fadH)
MLQ : ASQ50_05520(fadH) ASQ50_17800(fadH)
MSQ : BKP64_01575(fadH) BKP64_08910(fadH)
MARJ : MARI_02570(fadH_1) MARI_17560(fadH_3)
MALL : PBN92_06135 PBN92_14225
MSHE : MAALD49_13480(fadH1) MAALD49_28250(fadH)
MANP : EHN06_07110 EHN06_14810
MSAN : LPB19_09105 LPB19_15950
MNK : RE428_15140 RE428_31470
PSYC : DABAL43B_0225(fadH)
ABY : ABAYE1661(fadH) ABAYE2640(fadH)
ABC : ACICU_01106 ACICU_02015
ABN : AB57_1153(fadH) AB57_2239(fadH)
ABB : ABBFA_01531(fadH_2) ABBFA_02445(fadH_3)
ABZ : ABZJ_01256 ABZJ_02196
ABR : ABTJ_01694 ABTJ_02614
ABD : ABTW07_1277 ABTW07_2227
ABAD : ABD1_10850(fadH) ABD1_19250(fadH)
ABJ : BJAB07104_01292 BJAB07104_01724
ABAB : BJAB0715_01252 BJAB0715_02163
ABAJ : BJAB0868_01234 BJAB0868_02153
ABAZ : P795_12010 P795_7375
ABK : LX00_05400(fadH) LX00_10195(fadH)
ABAU : IX87_05595(fadH) IX87_20980(fadH)
ABAA : IX88_07385(fadH) IX88_11695(fadH)
ABW : BL01_00525(fadH) BL01_14625(fadH)
ABAL : ABLAC_18630 ABLAC_27770
ACC : BDGL_000348(fadH) BDGL_001385(fadH)
ANO : RR32_08335(fadH) RR32_12760(fadH)
ALC : OTEC02_07955(fadH) OTEC02_13545(fadH)
ACAL : BUM88_05180(fadH) BUM88_10915(fadH)
ASEI : I8T81_08225 I8T81_13645
ACD : AOLE_08305 AOLE_14005
ACI : ACIAD1155(fadH) ACIAD1654(fadH)
ATT : AMQ28_01110(fadH) AMQ28_12675(fadH)
AEI : AOY20_11945(fadH) AOY20_12860(fadH)
AJO : RZ95_06695(fadH) RZ95_11155(fadH)
ACV : AMD27_09180(fadH) AMD27_11015(fadH)
AHL : AHTJS_06075(fadH) AHTJS_08310(fadH)
AJN : BVL33_08090(fadH) BVL33_10160(fadH)
ASOL : BEN76_04480(fadH) BEN76_08010(fadH)
ASJ : AsACE_CH00921(fadH-1) AsACE_CH01605(fadH-2)
AID : CTZ23_04795 CTZ23_06345
ADV : DJ533_07265 DJ533_09760
ARJ : DOM24_07485(fadH) DOM24_09825(fadH)
AWU : BEN71_06280 BEN71_10825
ACUM : C9E88_008850 C9E88_011385
AGU : AS4_23600(fadH) AS4_30580(fadH)
ALW : FOB21_02525 FOB21_14925
ADS : FPL17_02110 FPL17_04630
ABER : BSR55_11520(fadH) BSR55_14740(fadH)
ATN : FM020_03875 FM020_08915
ACHI : CDG60_06300 CDG60_08085
ALJ : G8D99_04650 G8D99_06800
AWG : GFH30_04415 GFH30_07195
ASHA : G8E00_08950 G8E00_11165
ATG : J4G44_09350 J4G44_10850
AVB : RYU24_14190(fadH_1) RYU24_21010(fadH_2)
ABOU : ACBO_18130(fadH_1) ACBO_21980(fadH_2)
AGYL : FPL18_13585 FPL18_17390
ACIU : A3K93_04025(fadH) A3K93_06525(fadH)
AVZ : HWI77_08515 HWI77_10400
APOT : MZO21_04340 MZO21_06485
ATZ : M5E07_04565 M5E07_08945
ACOI : NQU59_10200 NQU59_13405
AVIV : LF296_09355 LF296_11415
ACIE : KIP84_07870 KIP84_13115
ASUE : F2A31_05880 F2A31_07495
ACIG : BS636_00260(fadH) BS636_12020(fadH)
MCAT : MC25239_01460(fadH)
MCUN : NCTC10297_02022(fadH)
MBAH : HYN46_04095 HYN46_07140 HYN46_08125
SVO : SVI_2061 SVI_2819(fadH)
SPSW : Sps_00806 Sps_01705
SBK : SHEWBE_2796(fadH) SHEWBE_3268(fadH)
SCAA : TUM17387_19350(fadH)
SPSH : FM037_13215 FM037_18155
SEUR : FM038_008625 FM038_012590
CPS : CPS_0925(fadH1) CPS_2449(fadH2)
COM : CMT41_03380(fadH) CMT41_09690(fadH)
LSD : EMK97_08700 EMK97_15325
THT : E2K93_05960 E2K93_16520
TPSY : RGQ13_09360 RGQ13_17330 RGQ13_18130
PLZ : S4054249_10915(fadH)
PART : PARC_a2220(fadH) PARC_b0610(fadH)
AMC : MADE_1012100(fadH) MADE_1015370(fadH)
AMH : I633_13220 I633_15830
AMAA : amad1_12855 amad1_15315
AMAL : I607_12100 I607_14615
AMAE : I876_12475 I876_14910
AMAO : I634_12330 I634_14855
AMAD : I636_12470 I636_14730
AMAI : I635_12835 I635_15285
AMAG : I533_12080 I533_14435
AMAC : MASE_11875 MASE_14440
AMB : AMBAS45_12425 AMBAS45_14910
AMG : AMEC673_12180 AMEC673_14715
AMK : AMBLS11_11615 AMBLS11_14240
ALT : ambt_02010 ambt_04885
AAL : EP13_12070(fadH) EP13_14680(fadH)
ASQ : AVL57_13665(fadH) AVL57_16555(fadH)
AAW : AVL56_12690(fadH) AVL56_15475(fadH)
ALE : AV939_12525(fadH) AV939_15495(fadH)
ALZ : AV940_12285(fadH) AV940_15245(fadH)
ALTE : ALT761_02644 ALT761_03211
ALTM : BM527_11850(fadH) BM527_14370(fadH)
ALTR : BM528_11950(fadH) BM528_14140(fadH)
GAG : Glaag_1968 Glaag_3023
GNI : GNIT_0302(fadH) GNIT_2210(fadH)
GPS : C427_2027(fadH) C427_3754
PMES : FX988_00276 FX988_01315
SALH : HMF8227_01322(fadH)
HMI : soil367_05415 soil367_12530
ALKM : NKI27_01175 NKI27_10770
ASEM : NNL22_01505 NNL22_07000 NNL22_17470
ALII : QR722_12640 QR722_14700
PMAW : MACH26_15020 MACH26_26250
FES : HER31_06920 HER31_18230
MVS : MVIS_0678(fadH) MVIS_1916
MYA : MORIYA_1062(fadH) MORIYA_2286(fadH)
MMAA : FR932_07825 FR932_14235
MOQ : HWV03_12770 HWV03_18755
MAGA : Mag101_05575(fadH) Mag101_16550(fadH)
HALC : EY643_05980 EY643_06810
LLG : 44548918_02103(fadH)
LJR : NCTC11533_00794(fadH)
LCJ : NCTC11976_01292(fadH)
LWA : SAMEA4504053_2586(fadH)
LSS : NCTC12082_01199(fadH)
LADL : NCTC12735_00407(fadH)
LANT : TUM19329_07640(fadH)
HAAH : HALA3H3_750023(fadH)
ALCA : ASALC70_03720(fadH_1)
MPON : MACH16_09310(fadH1)
TWAN : HUF19_07430 HUF19_10305
OAI : OLEAN_C13580 OLEAN_C32060
BSAN : CHH28_02075(fadH) CHH28_14545(fadH) CHH28_15875(fadH)
BMAR : HF888_06390 HF888_10030
OME : OLMES_2951 OLMES_4271
AEL : NCTC12917_00975(fadH)
CVI : CV_0169(fadH) CV_3946(FadH)
CVC : BKX93_14575(fadH) BKX93_17590
CHRI : DK842_08795(fadH) DK842_11875(fadH)
CHRB : DK843_14345(fadH) DK843_17310(fadH)
CNC : CNE_BB1p09070(fadH4)
BPS : BPSS1133(fadH) BPSS1898(fadH)
BPM : BURPS1710b_A0094(fadH) BURPS1710b_A0995(fadH)
BPL : BURPS1106A_A1515 BURPS1106A_A2575
BPD : BURPS668_A1598 BURPS668_A2719
BPZ : BP1026B_II1197(fadH) BP1026B_II2037
BPQ : BPC006_II1517 BPC006_II2538
BPSH : DR55_4194 DR55_5837
BPSO : X996_4334 X996_5635
BTE : BTH_II0478 BTH_II1273
BTHM : BTRA_4359 BTRA_5528
BTHA : DR62_3694(fadH) DR62_5387(fadH)
BTHL : BG87_4014 BG87_5603
BUU : WS70_23260(fadH) WS70_26975(fadH)
BSAV : WS86_24710(fadH) WS86_28670(fadH)
BHG : I6G56_22245 I6G56_27500
BUD : AQ610_24965(fadH) AQ610_29955(fadH)
BUR : Bcep18194_A4477 Bcep18194_B2335
BCM : Bcenmc03_1323 Bcenmc03_3759
BCH : Bcen2424_1344 Bcen2424_3764
BCJ : BCAL1336(fadH) BCAM0799(fadH)
BCEO : I35_1231(fadH) I35_4697(fadH)
BCEP : APZ15_10430(fadH) APZ15_20425(fadH)
BPYR : ABD05_12545(fadH) ABD05_17880(fadH)
BCON : NL30_08965(fadH) NL30_21810(fadH)
BSEM : WJ12_06595(fadH) WJ12_24000(fadH)
BMEC : WJ16_06480(fadH) WJ16_22880(fadH)
BSTG : WT74_22925(fadH) WT74_23855(fadH)
BSTL : BBJ41_05415(fadH) BBJ41_31655(fadH)
BARI : NLX30_06970 NLX30_18660
BAEN : L3V59_07215 L3V59_33790
BGD : bgla_1g20530 bgla_2g22460
PMEG : FNZ07_13665 FNZ07_26985
PLG : NCTC10937_01764(fadH)
LIMN : HKT17_07120 HKT17_10210
LTO : RGQ30_14920 RGQ30_22100
AXX : ERS451415_04803(fadH)
APES : FOC84_15170 FOC84_26965
RHG : EXZ61_08835 EXZ61_21355
RHOA : HZ993_01590 HZ993_04015
RLH : MIZ03_1218 MIZ03_4491
SMIO : CATMQ487_06820(fadH) CATMQ487_31870
RGU : A4W93_00465(fadH) A4W93_29140(fadH)
RUG : QC826_09565 QC826_28630
UPV : EJN92_08500 EJN92_11830
UPI : EJG51_011625 EJG51_014375
ABRE : pbN1_01300(fadH1) pbN1_41930(fadH2)
AZA : AZKH_2042(fadH) AZKH_2489(fadH)
THAG : CKCBHOJB_01792(fadH)
RBS : RHODOSMS8_03436(fadH)
VGO : GJW-30_1_01054(fadH_3)
RLI : RLO149_c001700(fadH)
PGA : PGA1_c25330(fadH1) PGA1_c29100(fadH2)
PGL : PGA2_c23640(fadH1) PGA2_c27030(fadH2)
PHP : PhaeoP97_00595(fadH2)
PPIC : PhaeoP14_02682(fadH2)
LEJ : ETW24_04740 ETW24_16640
LEV : ETW23_02290 ETW23_18740
MALG : MALG_03257 MALG_03753
SUAM : BOO69_01145(fadH) BOO69_21665(fadH)
SINL : DSM14862_03125(fadH)
SPSE : SULPSESMR1_03460(fadH) SULPSESMR1_04130(fadH)
RMM : ROSMUCSMR3_01433(fadH)
RID : RIdsm_04618(fadH_2) RIdsm_05474(fadH_4)
SAGU : CDO87_06745(fadH) CDO87_24165(fadH)
LALG : LentiSH36_02360(fadH2)
DAA : AKL17_4330 AKL17_4712
LVS : LOKVESSMR4R_02950(fadH)
MON : G8E03_00260 G8E03_02380
SFLA : SPHFLASMR4Y_03184(fadH)
PUK : PU629_11160 PU629_16820
MBO : BQ2027_MB1208C(fadH)
MMAE : MMARE11_40910(fadH)
MBAI : MB901379_03729(fadH_1)
MHEK : JMUB5695_01423(fadH_1) JMUB5695_01425(fadH_2)
MKY : IWGMT90018_48930(fadH)
MPHL : MPHLCCUG_04071(fadH_2)
MTHN : 4412656_04600(fadH_3)
MAUU : NCTC10437_04261(fadH)
MSAL : DSM43276_01191(fadH_2)
MTER : 4434518_01113(fadH)
NFR : ERS450000_02830(fadH_3)
NSL : BOX37_06225 BOX37_17075
NAD : NCTC11293_02164(fadH)
NGP : LTT66_02100 LTT66_05105
NSPU : IFM12276_35970 IFM12276_49930
RKO : JWS14_07270 JWS14_47410
RHOJ : JVH1_18930 JVH1_30280
SGRF : SGFS_012090 SGFS_098500
SAMB : SAM23877_1023(fadH)
SLE : sle_63530(sle_63530)
SPAV : Spa2297_03045(fadH)
STRO : STRMOE7_11830(fadH)
SHUN : DWB77_06632(fadH_2)
SAUH : SU9_008885 SU9_031565
SDD : D9753_04780 D9753_32660
SXT : KPP03845_106246(fadH_1)
ACRY : AC20117_01935(fadH)
JTE : ASJ30_06295(fadH) ASJ30_12160
JLI : EXU32_02230 EXU32_08195
JAY : H7A72_06340 H7A72_12560
AUS : IPK37_06275 IPK37_11890
NAQU : ENKNEFLB_04370(fadH)
LBI : LEPBI_I2046(fadH2) LEPBI_I2481(fadH)
LBF : LBF_1993(fadH-1) LBF_2407(fadH)
LAJ : A0128_09470 A0128_13690
LKB : LPTSP3_g21730(fadH1)
PHG : KCTC32516_00035(fadH)
KOS : KORDIASMS9_04123(fadH)
KAN : IMCC3317_42500(fadH)
PTAN : CRYO30217_01743(fadH)
» show all
Taxonomy
Reference
Authors
Dommes V, Luster W, Cvetanovic M, Kunau WH.
Title
Purification by affinity chromatography of 2,4-dienoyl-CoA reductases from bovine liver and Escherichia coli.
Journal
Reference
Authors
Dommes V, Kunau WH
Title
2,4-Dienoyl coenzyme A reductases from bovine liver and Escherichia coli. Comparison of properties.
Journal
J Biol Chem 259:1781-8 (1984)
Reference
Authors
You SY, Cosloy S, Schulz H
Title
Evidence for the essential function of 2,4-dienoyl-coenzyme A reductase in the beta-oxidation of unsaturated fatty acids in vivo. Isolation and characterization of an Escherichia coli mutant with a defective 2,4-dienoyl-coenzyme A reductase.
Journal
J Biol Chem 264:16489-95 (1989)
Reference
Authors
He XY, Yang SY, Schulz H
Title
Cloning and expression of the fadH gene and characterization of the gene product 2,4-dienoyl coenzyme A reductase from Escherichia coli.
Journal
Sequence
Reference
Authors
Liang X, Thorpe C, Schulz H
Title
2,4-Dienoyl-CoA reductase from Escherichia coli is a novel iron-sulfur flavoprotein that functions in fatty acid beta-oxidation.
Journal
Reference
Authors
Hubbard PA, Liang X, Schulz H, Kim JJ
Title
The crystal structure and reaction mechanism of Escherichia coli 2,4-dienoyl-CoA reductase.
Journal
Reference
Authors
Tu X, Hubbard PA, Kim JJ, Schulz H
Title
Two distinct proton donors at the active site of Escherichia coli 2,4-dienoyl-CoA reductase are responsible for the formation of different products.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 1.3.1.34
ExPASy - ENZYME nomenclature database: 1.3.1.34