KEGG ENZYME: 1.3.1.45

ENZYME: 1.3.1.45

Entry

EC 1.3.1.45                 Enzyme

Name

2'-hydroxyisoflavone reductase;
NADPH:2'-hydroxyisoflavone oxidoreductase;
isoflavone reductase;
2',7-dihydroxy-4',5'-methylenedioxyisoflavone reductase

Class

Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor

Sysname

vestitone:NADP+ oxidoreductase

Reaction(IUBMB)

vestitone + NADP+ = 2'-hydroxyformononetin + NADPH + H+ [RN:R06562]

Reaction(KEGG)

R06562;
(other) R06563 R07747 R07751

Substrate

vestitone [CPD:C00786];
NADP+ [CPD:C00006]

Product

2'-hydroxyformononetin [CPD:C02920];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

In the reverse reaction, a 2'-hydroxyisoflavone is reduced to an isoflavanone; 2'-hydroxypseudobaptigenin also acts. Involved in the biosynthesis of the pterocarpin phytoalexins medicarpin and maackiain.

History

EC 1.3.1.45 created 1990

Pathway

ec00943

Isoflavonoid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K05281

2'-hydroxyisoflavone reductase

Genes

GMX:	100778149 100781316(IFR) 100781863 547659

GSJ:	114372907 114374345 114420398 114420408

PVU:	PHAVU_002G032900g PHAVU_002G033000g

VRA:	106776923 106777314 106778034 106778035

VAR:	108335124 108335125

VUN:	114168785 114172980 114173087

VUM:	124830904 124833855

CCAJ:

109801392 109801470 109801488

APRC:

113868882 113869189

MTR:	11410094 11412853

TPRA:

123910352

CAM:	101508729(IFR)

PSAT:

127120219

VVO:

131652077

LJA:	130740702 130740704

ADU:

107487966

AIP:

107642895

AHF:	112749381 112800734

» show all

Reference

Authors

Tiemann K, Hinderer W, Barz W.

Title

Isolation of NADPH:isoflavone oxidoreductase, a new enzyme of pterocarpan biosynthesis in cell suspensions of Cicer arietinum.

Journal

FEBS Lett 213:324-328 (1987)

Other DBs

ExplorEnz - The Enzyme Database:

1.3.1.45

IUBMB Enzyme Nomenclature:

1.3.1.45

ExPASy - ENZYME nomenclature database:

1.3.1.45

BRENDA, the Enzyme Database:

1.3.1.45

CAS:	128449-69-4

DBGET integrated database retrieval system